FastQCFastQC Report
Thu 26 May 2016
SRR1512178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001138
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64770.3236658341403741No Hit
TATCAACGCAGAGTACTTTTTTTTT46790.2338169581508122No Hit
CTGTAGGACGTGGAATATGGCAAGA44460.2221735832311415No Hit
GGTATCAACGCAGAGTACTTTTTTT41990.20983060638496692No Hit
GTCCTACAGTGGACATTTCTAAATT38700.1933899611121272No Hit
CTTTAGGACGTGAAATATGGCGAGG36350.1816466430600988No Hit
GTCCTAAAGTGTGTATTTCTCATTT32650.16315716357392646No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22760.11373528462304949No Hit
CTGAAGGACCTGGAATATGGCGAGA20760.10374097138728064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG1006.6029315E-1014.24906355
TAGGACC7550.013.3368724
GATCGTC500.001502532713.29687
CCAACGA1403.6379788E-1212.89039919
GGTATCA15000.012.3308491
TCCAACG2150.011.48560718
TACACCG803.7739883E-410.6867975
GAACAGT5450.010.6304686
TGTAGGA25000.010.6013032
ATGCCGG2700.010.55354319
AGGACCT16400.010.4840665
GATATAC7550.010.4812641
CTGTAGG25250.010.3837411
CTAGGAC2750.010.3629553
GGCGAGG10450.010.3616619
AGGACGT22750.010.3553635
GTAGGAC23950.010.3124743
TAGACAG3700.010.2695955
CCCGTTT1856.002665E-1110.26779912
ACTGTTC7500.010.2575318
Produced by FastQC (version 0.11.5)