FastQCFastQC Report
Thu 26 May 2016
SRR1512177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929494
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30060.3234017648311877No Hit
GGTATCAACGCAGAGTACTTTTTTT18480.19881785143314534No Hit
TATCAACGCAGAGTACTTTTTTTTT17430.18752138260171664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1750.014.5307364
TCCAACG602.782968E-514.12786518
CGGTAGG450.006932735413.6748691
GGTATCA6400.012.9804411
GTATTAT1051.37817E-611.7213141
GGCGAGG2900.011.69202619
TTAGGAC4600.011.6702173
GTAGGAC5500.011.6441593
GTCCTAA2650.011.6107371
TGTAGGA5650.011.4360622
AGGACGT6200.011.2409225
GACGTGA3400.011.0800827
TAGGACG6300.011.0624954
CCCTTAC650.00602500511.0450861
GTTCTAG853.2500844E-410.8594541
GTTTAAG957.561477E-510.7959491
CTAGACC700.001602060610.7635084
TTACCCC700.001602060610.7635084
CTGTAGG6100.010.7605521
GTCTAGA1051.7616494E-510.7445381
Produced by FastQC (version 0.11.5)