FastQCFastQC Report
Thu 26 May 2016
SRR1512177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929494
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40680.437657478154781No Hit
GGTATCAACGCAGAGTACTTTTTTT24190.2602491247926291No Hit
TATCAACGCAGAGTACTTTTTTTTT24090.25917327061820733No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12450.13394384471551188No Hit
GTCCTACAGTGGACATTTCTAAATT10780.11597708000266813No Hit
CTGTAGGACGTGGAATATGGCAAGA9730.1046806111712394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC707.245484E-613.5731583
GTGGTAT2750.012.4822861
GGTATCA8400.012.1458751
TGTAGGA5950.011.9763162
GTCTAGA802.7574271E-511.918851
CCTAGAC752.0696534E-411.4014533
CTAATGC600.005867074211.0847473
TAGGACC1900.011.0014034
TGGTATC2850.011.0014032
GTGTAGG1053.3111646E-610.8972341
CCAGTAC700.001488840910.8585263
GGACGTG5900.010.62389856
TTGGACA909.492438E-510.5569014
GTAGGAC6400.010.5404063
AGGACCT3550.010.437955
TGGCGAG5150.010.14255718
AATGTCC1502.4489054E-810.1302638
GTTCTAA1053.9366234E-59.9891311
AGGACGT6200.09.9609485
CTTATAG1054.093645E-59.95364953
Produced by FastQC (version 0.11.5)