FastQCFastQC Report
Thu 26 May 2016
SRR1512176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780612
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66490.23912002106011193No Hit
GTCCTACAGTGGACATTTCTAAATT54020.19427377857824105No Hit
CTGTAGGACGTGGAATATGGCAAGA49070.17647194214798756No Hit
GTCCTAAAGTGTGTATTTCTCATTT44440.15982093150716462No Hit
GGTATCAACGCAGAGTACTTTTTTT40850.14691010468199087No Hit
TATCAACGCAGAGTACTTTTTTTTT40540.14579524219848003No Hit
CTTTAGGACGTGAAATATGGCGAGG40440.14543560913928302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7300.012.5189234
TCCAACG3650.012.13326618
GGCCTAC2150.011.4088461
TGTAGGA26500.011.1460482
CCAACGT1803.6379788E-1210.99308519
CGGTTTC2350.010.82562713
GTCCTAA13900.010.6615941
CCGGTTT2400.010.60009312
GTCCTAG4150.010.5898381
AGGACGT30000.010.5520335
CTGTAGG26650.010.5464051
GTAGGAC28000.010.5318433
GGCGAGG12700.010.38716619
GTCCTAC26650.010.2779511
GGACGTG29700.010.2462326
TAGGACG32150.010.0515094
CAACGCC750.002837878710.0493749
GCCGGTT2550.09.97512311
TTATACT2950.09.9005154
GACGTGG16150.09.8589867
Produced by FastQC (version 0.11.5)