FastQCFastQC Report
Thu 26 May 2016
SRR1512176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2780612
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86520.31115452281727907No Hit
GTCCTACAGTGGACATTTCTAAATT60350.2170385512254137No Hit
TATCAACGCAGAGTACTTTTTTTTT56540.20333653167000648No Hit
CTGTAGGACGTGGAATATGGCAAGA56260.20232955910425474No Hit
GGTATCAACGCAGAGTACTTTTTTT54460.19585616403870804No Hit
CTTTAGGACGTGAAATATGGCGAGG49340.17744295140781957No Hit
GTCCTAAAGTGTGTATTTCTCATTT48420.1741343272632068No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28100.10105688963436825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3400.012.56981718
TAGGACC7550.012.2058654
TACCGTA802.8769477E-511.8714937
CGCCCTA752.0806366E-411.39663316
CCTACCG600.005875607511.0838473
TCGCGCG600.005890570611.080069
AGGACGT31250.010.6708955
GGACGTG31000.010.6307326
TGTAGGA30150.010.6190662
GGTATCA24300.010.5177721
TTAGGAC18750.010.285813
GTAGGAC29300.010.2786333
CCAACGA2600.010.22885219
TAGGACG33950.010.1580564
CTGTAGG29900.010.1426441
GATATAC8000.010.1326771
CCGTATG750.002655192310.1303419
ACCGTAT750.002655192310.1303418
GTCCTAA14950.010.0788541
GGCGAGG14000.010.04097619
Produced by FastQC (version 0.11.5)