##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512176_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2780612 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.10526135972944 31.0 31.0 34.0 28.0 34.0 2 31.298212048282895 31.0 31.0 34.0 28.0 34.0 3 31.39008534811761 33.0 31.0 34.0 28.0 34.0 4 34.98006302209729 37.0 35.0 37.0 32.0 37.0 5 34.61994841423399 37.0 35.0 37.0 32.0 37.0 6 34.565119836927984 37.0 35.0 37.0 31.0 37.0 7 34.401060989451246 37.0 35.0 37.0 30.0 37.0 8 34.31706796920965 37.0 35.0 37.0 30.0 37.0 9 35.84729800489964 39.0 35.0 39.0 30.0 39.0 10 35.67758105050255 39.0 35.0 39.0 30.0 39.0 11 35.6602348691583 39.0 35.0 39.0 30.0 39.0 12 35.49431204353574 39.0 35.0 39.0 30.0 39.0 13 35.47292718293671 39.0 35.0 39.0 30.0 39.0 14 36.527411231772 40.0 36.0 41.0 30.0 41.0 15 36.45718928063319 40.0 36.0 41.0 30.0 41.0 16 36.36516925051032 40.0 36.0 41.0 29.0 41.0 17 36.30044213288298 40.0 36.0 41.0 29.0 41.0 18 36.246598950159175 39.0 36.0 41.0 29.0 41.0 19 36.27181570100395 40.0 36.0 41.0 29.0 41.0 20 36.16072253158657 39.0 36.0 41.0 29.0 41.0 21 36.05326669092991 39.0 36.0 41.0 28.0 41.0 22 36.148449693808416 39.0 36.0 41.0 29.0 41.0 23 36.04890434192185 39.0 36.0 41.0 29.0 41.0 24 35.93101770401624 39.0 36.0 41.0 29.0 41.0 25 35.49384272239349 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0337648457227715 1101 2 0.07077055866318815 1101 3 0.1334727377242224 1101 4 0.12425713674957706 1101 5 0.09484391790307001 1101 6 0.08059893339862612 1101 7 0.13709762605299858 1101 8 0.03634758657424442 1101 9 0.08605978559076277 1101 10 0.1765266108334984 1101 11 0.10035236713805773 1101 12 0.27002702705792103 1101 13 0.07536297980503548 1101 14 -0.07263601237248452 1101 15 0.07230766842265979 1101 16 0.09338419355928096 1101 17 -0.027032462415157 1101 18 0.04413021156465646 1101 19 0.13024590130481073 1101 20 0.056040244150096896 1101 21 -0.05574681665355996 1101 22 0.003247451601723128 1101 23 0.004531882668842968 1101 24 -0.017433424126224395 1101 25 0.08851949968784112 1102 1 0.2914696292105319 1102 2 0.2759186848672819 1102 3 0.20613253889060346 1102 4 0.2195169906329184 1102 5 0.26331840742845714 1102 6 0.4012500723846628 1102 7 0.29564826315205295 1102 8 0.4078818114850833 1102 9 0.4912454130724413 1102 10 0.36033020865074405 1102 11 0.465638633294148 1102 12 0.5451047384656107 1102 13 0.6189941024841659 1102 14 0.6834046519226931 1102 15 0.6780641145310895 1102 16 0.7625934161070802 1102 17 0.73860422551504 1102 18 0.9245770608041397 1102 19 0.7482757163574263 1102 20 0.8602357764261725 1102 21 0.7286334401533452 1102 22 0.7465086333991593 1102 23 0.8150509910298567 1102 24 0.8529202021627853 1102 25 0.7197980242892186 1103 1 0.46732855735351464 1103 2 0.3742581447622477 1103 3 0.3321959984182712 1103 4 0.33473613986529926 1103 5 0.3695214400024156 1103 6 0.40509055271499506 1103 7 0.45612897855278334 1103 8 0.47197566784214473 1103 9 0.5940853039495408 1103 10 0.6799585569080051 1103 11 0.5978760970457415 1103 12 0.5599457786284106 1103 13 0.5332958966139216 1103 14 0.5964348265963437 1103 15 0.7117975750362433 1103 16 0.7905401638951517 1103 17 0.5765293205236475 1103 18 0.7044690042521751 1103 19 0.6322145968702202 1103 20 0.5316298185054293 1103 21 0.8352493861961605 1103 22 0.8644669629064765 1103 23 0.9587771648267349 1103 24 0.7167182951779125 1103 25 0.7844321634061018 1104 1 0.18255648014215353 1104 2 0.17118181855948933 1104 3 0.23203534999938213 1104 4 0.15266470820572664 1104 5 0.28840024601603176 1104 6 0.2809395887107087 1104 7 0.30435433688294466 1104 8 0.27727820340210485 1104 9 0.387551395983337 1104 10 0.47346390085717616 1104 11 0.3879221526264516 1104 12 0.5132109691196618 1104 13 0.42425692003082105 1104 14 0.6947733897259383 1104 15 0.7830523165538494 1104 16 0.8087200582676743 1104 17 0.8165121179450807 1104 18 0.8285630814225797 1104 19 0.7376788048120773 1104 20 0.7245785816718922 1104 21 0.7359727591758229 1104 22 0.7689408848440848 1104 23 0.7836846099480113 1104 24 0.8773650074602415 1104 25 0.8589374186118164 1105 1 -0.002830340993519087 1105 2 0.09013485485139938 1105 3 0.13204322677694336 1105 4 0.02877960157282189 1105 5 0.045680776213735896 1105 6 0.0466662475134072 1105 7 0.09484412973193201 1105 8 0.04547987196157521 1105 9 0.0014367002218094171 1105 10 0.1735448916954141 1105 11 0.22597629216123494 1105 12 0.09512328055043184 1105 13 0.018193255907590355 1105 14 0.17162924908785726 1105 15 0.24803551184688644 1105 16 0.09262423383803053 1105 17 -0.14708907339694122 1105 18 0.047680212672744915 1105 19 0.12000743275427084 1105 20 0.13638008029057147 1105 21 0.10774197672913033 1105 22 0.2267460578285494 1105 23 0.2283875730984093 1105 24 0.3062490958755575 1105 25 0.3550314151899485 1106 1 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-0.597232567692906 2314 15 -0.5932949166522121 2314 16 -0.5650296542500755 2314 17 -0.450821268450035 2314 18 -0.5856404157498005 2314 19 -0.4799666588915841 2314 20 -0.7790544535437007 2314 21 -0.5955990850094892 2314 22 -0.5547465417814195 2314 23 -0.5658457932953027 2314 24 -0.5456099953509579 2314 25 -0.7835866778378389 2315 1 -0.3267853459634509 2315 2 -0.34560043814613906 2315 3 -0.31108353221749496 2315 4 -0.35047269011843696 2315 5 -0.4028613768144993 2315 6 -0.4838651592251395 2315 7 -0.588716579616495 2315 8 -0.6213465878847941 2315 9 -0.7106131733400503 2315 10 -0.6818598150785462 2315 11 -0.6900767099828826 2315 12 -0.7000516953615801 2315 13 -0.6676955623147336 2315 14 -0.8554905653298945 2315 15 -0.8683154244427627 2315 16 -1.07670123583015 2315 17 -0.9192479998533614 2315 18 -0.7995977731905555 2315 19 -0.9051905790821664 2315 20 -0.9211984246546052 2315 21 -0.8940689068525742 2315 22 -0.8996633989444618 2315 23 -0.7160769975641301 2315 24 -0.8057414860064682 2315 25 -0.9960304319113078 2316 1 -0.7755092511052233 2316 2 -0.6836947093396653 2316 3 -0.7058775275855567 2316 4 -0.669678143903603 2316 5 -0.7316380948935333 2316 6 -0.8490714453116368 2316 7 -0.7961540162892149 2316 8 -0.8385253678194999 2316 9 -0.936650433902642 2316 10 -1.0986178204503076 2316 11 -1.06026836043241 2316 12 -1.0918147267075753 2316 13 -0.975471749650886 2316 14 -1.3014733041239879 2316 15 -1.2951800608838937 2316 16 -1.3213023343070773 2316 17 -1.2010916679299797 2316 18 -1.1952333176845045 2316 19 -1.120182356588316 2316 20 -1.0877347845626133 2316 21 -1.1013529507916502 2316 22 -1.0714559227072655 2316 23 -0.9601738903058177 2316 24 -1.0698950315297324 2316 25 -1.0751391658482916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33583.0 3 2.0 4 614.0 5 5681.0 6 7339.0 7 6038.0 8 4694.0 9 4436.0 10 4218.0 11 4023.0 12 4048.0 13 3761.0 14 3852.0 15 3778.0 16 3888.0 17 4086.0 18 4487.0 19 5051.0 20 6141.0 21 7663.0 22 10259.0 23 12888.0 24 16148.0 25 19462.0 26 22742.0 27 26147.0 28 30957.0 29 37976.0 30 48733.0 31 63116.0 32 82195.0 33 107213.0 34 140711.0 35 186949.0 36 268813.0 37 464238.0 38 1124682.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.84606250115119 19.42404808881001 13.838823465253242 24.891065944785556 2 14.758261850268934 20.42582712007285 41.02348691583004 23.79242411382818 3 18.733322016879736 24.39743480931536 30.065179895648868 26.804063278156033 4 12.723422038026161 17.206212157611343 37.8308084695024 32.239557334860095 5 13.291444342755836 37.628933478383495 35.702234175430114 13.377388003430552 6 31.915348131993966 37.106795194726914 17.899944328802437 13.077912344476683 7 28.13524504677388 31.610271407877118 21.958223585311433 18.296259960037574 8 24.917787882667554 37.4802022000912 20.344729865223915 17.257280052017325 9 26.062356056868058 15.733478816893548 20.400041429728418 37.804123696509976 10 15.270487216483279 29.114310087131894 33.971046661670165 21.64415603471466 11 34.13748484146655 22.49335757739663 24.607136846133155 18.762020735003663 12 23.428871054285892 24.51859518695884 31.63246076762957 20.420072991125696 13 28.909714839754702 21.882269083209017 25.966837516345322 23.241178560690955 14 20.921833035317405 22.401327477548108 27.487438017242248 29.18940146989224 15 22.855903664373166 31.14785522036156 23.43685490820007 22.559386207065206 16 22.076291118645823 28.197821199074163 27.914286495203218 21.8116011870768 17 21.52349914335405 28.804666023163243 27.968950720201164 21.702884113281538 18 21.32839820873966 27.47150627271982 30.250750554194543 20.94934496434598 19 23.402761694188186 26.900193194879403 27.66592390452174 22.031121206410674 20 23.396000592675282 27.443778563855727 28.320312219036676 20.83990862443232 21 22.733376681104737 26.79838107582072 27.504736367389626 22.963505875684923 22 22.910963485736232 27.962836958194814 27.71871803761186 21.40748151845709 23 22.272974438720684 27.61226665208954 28.057312562845876 22.057446346343898 24 22.98918367611159 26.75590841152955 28.633696466820975 21.621211445537888 25 22.664448780312362 27.243928536105095 28.38084105847128 21.71078162511127 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 626.0 1 626.0 2 705.0 3 784.0 4 784.0 5 784.0 6 1993.5 7 3203.0 8 3203.0 9 3203.0 10 4530.5 11 5858.0 12 5858.0 13 5858.0 14 9342.5 15 12827.0 16 12827.0 17 12827.0 18 22822.5 19 32818.0 20 32818.0 21 32818.0 22 52470.5 23 72123.0 24 72123.0 25 72123.0 26 103772.5 27 135422.0 28 135422.0 29 135422.0 30 175127.5 31 214833.0 32 214833.0 33 214833.0 34 240792.5 35 266752.0 36 266752.0 37 266752.0 38 285318.5 39 303885.0 40 303885.0 41 303885.0 42 324406.0 43 344927.0 44 344927.0 45 344927.0 46 351378.0 47 357829.0 48 357829.0 49 357829.0 50 347167.0 51 336505.0 52 336505.0 53 336505.0 54 300663.0 55 264821.0 56 264821.0 57 264821.0 58 226940.5 59 189060.0 60 189060.0 61 189060.0 62 155171.5 63 121283.0 64 121283.0 65 121283.0 66 94255.5 67 67228.0 68 67228.0 69 67228.0 70 49264.0 71 31300.0 72 31300.0 73 31300.0 74 21866.0 75 12432.0 76 12432.0 77 12432.0 78 8361.5 79 4291.0 80 4291.0 81 4291.0 82 2823.0 83 1355.0 84 1355.0 85 1355.0 86 864.0 87 373.0 88 373.0 89 373.0 90 220.5 91 68.0 92 68.0 93 68.0 94 38.0 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42202939496772657 2 0.0 3 0.0 4 0.0 5 0.03179156243301834 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0067251382069846485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2780612.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.80818912695979 #Duplication Level Percentage of deduplicated Percentage of total 1 81.31916166768721 55.141050940083524 2 12.526449458559991 16.987917079506776 3 3.237250081804762 6.5853619739485 4 1.1911064574174066 3.2306708773961033 5 0.5627657870734023 1.908006446202782 6 0.3046587009027097 1.2395012885990886 7 0.18891179041667444 0.8966836489020514 8 0.11667141789990312 0.6329022056533753 9 0.0906897630005533 0.5534557741278611 >10 0.41480252408617696 4.9629053275177855 >50 0.025192710779180064 1.1743855702418018 >100 0.01841524254821086 2.56056920847828 >500 0.002015231315001846 1.018827563601791 >1k 0.0016440044936824004 1.9781408104948506 >5k 2.6516201511006456E-4 1.1296212852455916 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8652 0.31115452281727907 No Hit GTCCTACAGTGGACATTTCTAAATT 6035 0.2170385512254137 No Hit TATCAACGCAGAGTACTTTTTTTTT 5654 0.20333653167000648 No Hit CTGTAGGACGTGGAATATGGCAAGA 5626 0.20232955910425474 No Hit GGTATCAACGCAGAGTACTTTTTTT 5446 0.19585616403870804 No Hit CTTTAGGACGTGAAATATGGCGAGG 4934 0.17744295140781957 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4842 0.1741343272632068 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2810 0.10105688963436825 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.5963305919704006E-5 0.0 10 0.0 0.0 0.0 3.5963305919704006E-5 0.0 11 0.0 0.0 0.0 3.5963305919704006E-5 0.0 12 7.192661183940801E-5 0.0 0.0 3.5963305919704006E-5 0.0 13 7.192661183940801E-5 0.0 0.0 3.5963305919704006E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 340 0.0 12.569817 18 TAGGACC 755 0.0 12.205865 4 TACCGTA 80 2.8769477E-5 11.871493 7 CGCCCTA 75 2.0806366E-4 11.396633 16 CCTACCG 60 0.0058756075 11.083847 3 TCGCGCG 60 0.0058905706 11.08006 9 AGGACGT 3125 0.0 10.670895 5 GGACGTG 3100 0.0 10.630732 6 TGTAGGA 3015 0.0 10.619066 2 GGTATCA 2430 0.0 10.517772 1 TTAGGAC 1875 0.0 10.28581 3 GTAGGAC 2930 0.0 10.278633 3 CCAACGA 260 0.0 10.228852 19 TAGGACG 3395 0.0 10.158056 4 CTGTAGG 2990 0.0 10.142644 1 GATATAC 800 0.0 10.132677 1 CCGTATG 75 0.0026551923 10.130341 9 ACCGTAT 75 0.0026551923 10.130341 8 GTCCTAA 1495 0.0 10.078854 1 GGCGAGG 1400 0.0 10.040976 19 >>END_MODULE