FastQCFastQC Report
Thu 26 May 2016
SRR1512175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2985123
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65320.21881845404695216No Hit
GGTATCAACGCAGAGTACTTTTTTT41420.1387547514792523No Hit
TATCAACGCAGAGTACTTTTTTTTT40320.13506981119370962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC951.1501579E-611.9011257
ACCGTAT1053.8319613E-610.7673248
GTAGGAC9550.010.5562773
GTCTTAG3900.010.479951
GATATAC7900.010.2179511
GTCCTAA6550.010.1399511
TAGGACG9650.010.0563484
GGACCGT1607.0776878E-910.0105826
GGTATCA22450.09.9221071
GGACGTG9250.09.8800786
AGGACGT9750.09.8565735
TGTAGGA11600.09.6894362
GTATAGA3800.09.6801631
TAGGACC4650.09.5230324
AATCCCG3500.09.4228319
TTAGGGC2500.09.4217243
GTCCTAC9700.09.2698931
GCACCGT1651.2111377E-79.1362176
TCCTACA11550.09.1121112
TCCAACG2800.09.08629918
Produced by FastQC (version 0.11.5)