FastQCFastQC Report
Thu 26 May 2016
SRR1512175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512175_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2985123
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84480.28300341392967726No Hit
TATCAACGCAGAGTACTTTTTTTTT55500.1859219871341985No Hit
GGTATCAACGCAGAGTACTTTTTTT54100.18123206313441692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3500.012.4819518
CGATCGC600.005891014411.07999210
GACGCTC1053.481653E-610.853878
GTAGGAC9250.010.6819753
AATCCCG4450.010.67129619
AGGGACG1701.382432E-1010.6185365
TAGAACC2700.010.5564394
TAGGACT3700.010.5279094
GGTATCA24900.010.4942571
GATATAC8250.010.1725351
ACTGTTC10650.010.0767748
TATACAC10950.010.064773
CCAACGA2750.010.01549719
TGTAGAA11600.09.828412
AGAACAG10450.09.8190045
TGTAGGA10850.09.8072732
TAGGACC4400.09.7167234
GTAGAAC10350.09.6384883
TAGAACA11600.09.58274
ATACACT10950.09.5441784
Produced by FastQC (version 0.11.5)