FastQCFastQC Report
Thu 26 May 2016
SRR1512174_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512174_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772250
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66610.24027414554964377No Hit
GGTATCAACGCAGAGTACTTTTTTT43140.15561367120569936No Hit
TATCAACGCAGAGTACTTTTTTTTT41860.15099648300117233No Hit
GTCCTACAGTGGACATTTCTAAATT36410.13133736134908466No Hit
CTGTAGGACGTGGAATATGGCAAGA34520.12451979439083777No Hit
GTCCTAAAGTGTGTATTTCTCATTT30180.10886464063486337No Hit
CTTTAGGACGTGAAATATGGCGAGG28390.10240779150509514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5450.011.9277414
GTCCTAC18050.011.2733541
CCCGTAC951.4915629E-510.9095316
GTACTAG2151.8189894E-1210.4631271
ACCGTAT750.00283897610.0489038
TGTAGGA21200.09.9841652
ACAACCG951.78906E-49.9170385
GTCCTAA11900.09.8816191
TCCTACA20250.09.8466122
GGACGTG21300.09.8192656
GGTATCA20950.09.761651
AGGACGT22400.09.7155985
GGCGAGG9400.09.62250519
GTATAGC3950.09.57815551
GACGTGA9650.09.567637
GTCTTAC2256.184564E-119.5436391
GTAGGAC21900.09.4642063
GTCCTAT2601.8189894E-129.4387651
ACGGGTG900.00120225579.4211865
TATACTG3300.09.4211865
Produced by FastQC (version 0.11.5)