##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512174_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2772250 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.536113265398143 31.0 28.0 33.0 16.0 34.0 2 25.619739922445667 31.0 25.0 34.0 2.0 34.0 3 27.28839570745784 31.0 26.0 34.0 16.0 34.0 4 31.75881684552259 35.0 32.0 37.0 19.0 37.0 5 32.16074199657318 35.0 33.0 37.0 22.0 37.0 6 32.35616484804761 35.0 33.0 37.0 23.0 37.0 7 32.33427649021553 35.0 33.0 37.0 22.0 37.0 8 32.330699972946164 35.0 33.0 37.0 22.0 37.0 9 33.754957525475696 38.0 34.0 39.0 23.0 39.0 10 33.609219586978085 38.0 34.0 39.0 22.0 39.0 11 33.615604653259986 38.0 34.0 39.0 22.0 39.0 12 33.482563621607 38.0 33.0 39.0 19.0 39.0 13 33.470573721706195 38.0 33.0 39.0 19.0 39.0 14 34.441158625665075 39.0 34.0 41.0 19.0 41.0 15 34.355553431328346 39.0 33.0 41.0 18.0 41.0 16 34.28803318604022 39.0 33.0 41.0 17.0 41.0 17 34.2564635224096 39.0 33.0 41.0 17.0 41.0 18 34.19912886644423 39.0 33.0 41.0 17.0 41.0 19 34.208170258815045 39.0 34.0 41.0 18.0 41.0 20 34.10580214627108 39.0 34.0 41.0 17.0 41.0 21 34.01815997835693 39.0 34.0 41.0 15.0 41.0 22 33.902957886193526 39.0 34.0 41.0 10.0 41.0 23 33.767237081792764 39.0 33.0 41.0 8.0 41.0 24 33.6199581567319 39.0 33.0 41.0 2.0 41.0 25 33.47087888898909 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.613076232502376 1101 2 3.4029551910828246 1101 3 2.0322826972222465 1101 4 1.074827871349271 1101 5 0.6597093792451219 1101 6 0.7092528228094039 1101 7 0.43482621834467494 1101 8 0.5255993395589655 1101 9 0.4372823335505345 1101 10 0.25461487261282656 1101 11 0.5656421926589843 1101 12 0.4703895651310859 1101 13 0.38765441164098036 1101 14 0.49008977978441237 1101 15 0.5999345640121447 1101 16 0.8694078574132078 1101 17 0.5836958543437802 1101 18 0.5884140884392934 1101 19 0.45637399030392345 1101 20 0.4946864464551126 1101 21 0.6058722145934254 1101 22 0.580613901836756 1101 23 0.43665900868664664 1101 24 0.4364927095593245 1101 25 0.44830240731937465 1102 1 1.1403684771144498 1102 2 3.94570548325267 1102 3 2.3284740615865758 1102 4 1.457615781616731 1102 5 1.123227300963272 1102 6 1.0587882235641217 1102 7 1.018266070705451 1102 8 0.7837323643924208 1102 9 0.8163507740915108 1102 10 0.8639990926865693 1102 11 0.9141250736701068 1102 12 0.8729771865734008 1102 13 0.7938621847361702 1102 14 1.0308725982298697 1102 15 1.161390179772397 1102 16 1.1293027943163239 1102 17 1.1643119884153066 1102 18 1.1646638474237605 1102 19 1.2260767837231157 1102 20 1.1321211755142997 1102 21 1.0926299359440605 1102 22 1.1367606438970128 1102 23 0.9784419333756915 1102 24 1.1300369560759123 1102 25 1.0144051960166323 1103 1 1.2566575349704365 1103 2 3.9456039434820553 1103 3 2.5586868539527003 1103 4 1.67496203447768 1103 5 1.2646687619396246 1103 6 1.064270174832508 1103 7 0.9218065785687983 1103 8 1.0878273781017853 1103 9 0.889493504763621 1103 10 1.0556563817541402 1103 11 0.932287532816801 1103 12 1.045518978264333 1103 13 1.0530453653236833 1103 14 1.2070497828864504 1103 15 1.3987635447989035 1103 16 1.4509631415404627 1103 17 1.45023766399499 1103 18 1.3829242573064704 1103 19 1.351064465109559 1103 20 1.3986434832336414 1103 21 1.3478563554259395 1103 22 1.4141686431074234 1103 23 1.3441128174072503 1103 24 1.4785299727866033 1103 25 1.4764262561725943 1104 1 1.129103925159285 1104 2 3.894116239771961 1104 3 2.4695202412013657 1104 4 1.4778332055905636 1104 5 1.244682708038674 1104 6 1.1003503145810498 1104 7 0.8787871762382196 1104 8 0.8238787630069311 1104 9 0.9251157236311158 1104 10 0.9567009014404277 1104 11 0.7666409442195317 1104 12 0.8437297081341484 1104 13 1.068746228687857 1104 14 1.0389741288934786 1104 15 0.6757488870174839 1104 16 0.8450360494458948 1104 17 0.9087940270824006 1104 18 0.8378704498057701 1104 19 0.8883975971589848 1104 20 0.9988857427892341 1104 21 1.0359482559054243 1104 22 1.2390839960371878 1104 23 1.1012192175165225 1104 24 1.0762906893573003 1104 25 1.0390866542834019 1105 1 1.044252055533871 1105 2 3.754030301539377 1105 3 2.397373219529264 1105 4 1.5580136317515283 1105 5 1.0712719861685542 1105 6 0.8023525981772153 1105 7 0.6869325541623539 1105 8 0.6917332857960048 1105 9 0.7500766984067511 1105 10 0.7729191227727483 1105 11 0.8887071574765244 1105 12 0.684505689556488 1105 13 0.8117644367819352 1105 14 0.9452134762483126 1105 15 0.922487040172868 1105 16 0.7306016807719757 1105 17 0.9429495101953691 1105 18 1.0010300864161152 1105 19 1.0625656565986787 1105 20 1.036424147471159 1105 21 1.0613422314036498 1105 22 1.1164863226576287 1105 23 0.9735714318877342 1105 24 1.0290643761516094 1105 25 1.1079072926974618 1106 1 0.4546495071482788 1106 2 3.081986848438504 1106 3 1.6163806600911599 1106 4 0.8338979012572878 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0.7005638619025447 1107 17 0.6309429282575181 1107 18 0.7061501795087182 1107 19 0.6892615965248581 1107 20 0.7569599753953042 1107 21 0.8583012670224832 1107 22 0.7361630440525602 1107 23 0.8900308220584634 1107 24 0.7752324882991033 1107 25 0.9480298403801442 1108 1 0.9017631011892462 1108 2 3.3591149668361524 1108 3 2.0400159512764553 1108 4 1.3247428586433756 1108 5 0.9430239545709043 1108 6 0.7378950969877636 1108 7 0.7493868208660359 1108 8 0.7185429310449507 1108 9 0.8194310810111247 1108 10 0.5668602415085076 1108 11 0.6920534672832943 1108 12 0.7278187978931854 1108 13 0.7107048175925783 1108 14 0.771764692895907 1108 15 0.8749651784961614 1108 16 0.8561730567081867 1108 17 0.8460978702986992 1108 18 0.7322524722776791 1108 19 0.8737381009266159 1108 20 0.8786395018867879 1108 21 0.8736778108503245 1108 22 0.9588103017150047 1108 23 0.8753027693486288 1108 24 0.9003738474979244 1108 25 0.9794045995287846 1109 1 0.7918580808364659 1109 2 3.324108515370476 1109 3 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-2.1990423717345173 2315 22 -1.8745108207032644 2315 23 -1.9931562787472252 2315 24 -2.0037028569479602 2315 25 -1.8239594171205695 2316 1 -1.3243555912893328 2316 2 -0.11633392348398175 2316 3 -0.48721247666835765 2316 4 -1.066201207121889 2316 5 -1.2011665972766856 2316 6 -1.2059915280299975 2316 7 -1.4066095291653298 2316 8 -1.416088692779656 2316 9 -1.4513712604664377 2316 10 -1.4848620290602028 2316 11 -1.9872686998266467 2316 12 -1.5915263249663347 2316 13 -1.4136195145456725 2316 14 -1.6401100926306285 2316 15 -2.0136683005586633 2316 16 -1.6291170937807422 2316 17 -1.839225328285437 2316 18 -2.298214046287857 2316 19 -1.928558674971491 2316 20 -2.0408254481174026 2316 21 -1.838393995588362 2316 22 -1.750223126010745 2316 23 -1.8422463251980545 2316 24 -1.6646698522696965 2316 25 -1.5706231221742257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 148443.0 3 3.0 4 1305.0 5 6328.0 6 7460.0 7 6568.0 8 6145.0 9 5861.0 10 5947.0 11 5606.0 12 5414.0 13 5581.0 14 5636.0 15 5639.0 16 5930.0 17 6552.0 18 7212.0 19 9214.0 20 11859.0 21 15218.0 22 18885.0 23 22663.0 24 25797.0 25 29166.0 26 32427.0 27 36397.0 28 42159.0 29 49925.0 30 61301.0 31 76234.0 32 96203.0 33 123147.0 34 161634.0 35 219936.0 36 306022.0 37 381720.0 38 816713.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.76551537559744 18.95579403011994 13.412534944539633 24.86615564974299 2 16.068133527996082 20.68734116604239 40.02500233932004 23.21952296664149 3 18.72352782036252 24.260618631075843 29.764775904049056 27.251077644512577 4 12.397943908377671 16.88321760303003 38.125529804310574 32.593308684281716 5 13.416899630264226 37.52994859770944 35.606637208044006 13.446514563982326 6 31.7804523049009 36.90196934253646 18.130648625933134 13.1869297266295 7 28.079370576394453 31.755265037553297 22.00381665620513 18.161547729847115 8 25.07681486157453 36.965821985751646 20.475209667237802 17.482153485436015 9 26.09775453151772 16.04231220128055 20.423518802416808 37.436414464784924 10 15.213651473312279 29.083619061704702 33.914662058939705 21.788067406043314 11 33.98524849117539 22.843808381168543 24.41848343061499 18.752459697041076 12 23.252101907335717 24.75305204015231 31.43843038347274 20.556415669039232 13 28.620939669943184 21.949535575795835 26.024528812336552 23.40499594192443 14 21.114887836311606 22.208731341372314 27.664313698330194 29.012067123985886 15 23.074398052123726 30.498115249346196 23.652231941563713 22.775254756966362 16 22.338641897375776 28.204599152313104 27.456686806745424 22.000072143565696 17 21.65187420303761 28.613524417935643 27.817274447244024 21.917326931782725 18 21.564848732347418 27.532834002050528 29.635775804534966 21.266541461067096 19 23.239836215009824 27.130108496193056 27.759470826294624 21.870584462502492 20 23.317485796735504 27.30861213815493 28.256217873568403 21.117684191541166 21 22.936217339388975 26.800946585042944 27.583177913977643 22.679658161590435 22 22.98170298618318 27.715486719758054 27.65214354947567 21.650666744583095 23 22.473340456493055 27.467608501329998 27.875446670451897 22.18360437172505 24 22.877735577880458 26.834817705101603 28.40451920496163 21.88292751205631 25 22.788583739075445 27.195056710103348 28.083486737941637 21.932872812879566 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 619.0 1 619.0 2 739.0 3 859.0 4 859.0 5 859.0 6 2097.5 7 3336.0 8 3336.0 9 3336.0 10 4869.0 11 6402.0 12 6402.0 13 6402.0 14 10132.5 15 13863.0 16 13863.0 17 13863.0 18 24103.0 19 34343.0 20 34343.0 21 34343.0 22 53409.0 23 72475.0 24 72475.0 25 72475.0 26 102493.5 27 132512.0 28 132512.0 29 132512.0 30 168914.5 31 205317.0 32 205317.0 33 205317.0 34 233103.0 35 260889.0 36 260889.0 37 260889.0 38 282401.0 39 303913.0 40 303913.0 41 303913.0 42 324006.5 43 344100.0 44 344100.0 45 344100.0 46 351066.5 47 358033.0 48 358033.0 49 358033.0 50 347981.0 51 337929.0 52 337929.0 53 337929.0 54 302859.5 55 267790.0 56 267790.0 57 267790.0 58 230029.5 59 192269.0 60 192269.0 61 192269.0 62 157365.5 63 122462.0 64 122462.0 65 122462.0 66 94236.5 67 66011.0 68 66011.0 69 66011.0 70 48373.5 71 30736.0 72 30736.0 73 30736.0 74 21489.0 75 12242.0 76 12242.0 77 12242.0 78 8269.0 79 4296.0 80 4296.0 81 4296.0 82 2814.5 83 1333.0 84 1333.0 85 1333.0 86 856.5 87 380.0 88 380.0 89 380.0 90 241.0 91 102.0 92 102.0 93 102.0 94 65.5 95 29.0 96 29.0 97 29.0 98 19.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 7.8668590495085216 3 0.0 4 0.0 5 0.0 6 1.8035891423933626E-4 7 0.006853638741094779 8 0.0 9 0.0 10 0.0034268193705473894 11 0.0021282351880241683 12 2.1643069708720354E-4 13 0.0 14 2.525024799350708E-4 15 0.0 16 0.0 17 1.8035891423933626E-4 18 0.009955812066011363 19 0.002849670844981513 20 0.0 21 0.006492920912616106 22 0.0017675173595454955 23 0.0025971683650464423 24 0.009306519974749752 25 0.006096131301289566 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2772250.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.1261619161139 #Duplication Level Percentage of deduplicated Percentage of total 1 82.88211021805138 59.779685015363725 2 11.54812828600746 16.65844341169258 3 3.0147204245140626 6.5232064041095095 4 1.063915608048252 3.06944597644676 5 0.5119493808695506 1.8462471968725735 6 0.26834716340396597 1.1612910574442596 7 0.1679060040061279 0.8477290942143556 8 0.11091152136953061 0.6399697878929035 9 0.07216538609797675 0.4684511090197392 >10 0.32782297775520136 4.014892972355419 >50 0.017622166212747026 0.8730821844273826 >100 0.011520426068437428 1.5721936803588414 >500 0.0018192237445579025 0.8500456094054002 >1k 0.0010106798578342902 1.4525348434589282 >5k 5.053399289171451E-5 0.2427816569376674 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6661 0.24027414554964377 No Hit GGTATCAACGCAGAGTACTTTTTTT 4314 0.15561367120569936 No Hit TATCAACGCAGAGTACTTTTTTTTT 4186 0.15099648300117233 No Hit GTCCTACAGTGGACATTTCTAAATT 3641 0.13133736134908466 No Hit CTGTAGGACGTGGAATATGGCAAGA 3452 0.12451979439083777 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3018 0.10886464063486337 No Hit CTTTAGGACGTGAAATATGGCGAGG 2839 0.10240779150509514 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.6071782847867254E-5 2 0.0 0.0 0.0 0.0 3.6071782847867254E-5 3 0.0 0.0 0.0 0.0 3.6071782847867254E-5 4 0.0 0.0 0.0 0.0 3.6071782847867254E-5 5 0.0 0.0 0.0 0.0 3.6071782847867254E-5 6 0.0 0.0 0.0 0.0 7.214356569573451E-5 7 0.0 0.0 0.0 0.0 7.214356569573451E-5 8 0.0 0.0 0.0 0.0 7.214356569573451E-5 9 0.0 0.0 0.0 0.0 1.0821534854360177E-4 10 0.0 0.0 0.0 0.0 1.0821534854360177E-4 11 0.0 0.0 0.0 0.0 1.4428713139146902E-4 12 0.0 0.0 0.0 0.0 1.4428713139146902E-4 13 0.0 0.0 0.0 0.0 1.4428713139146902E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 545 0.0 11.927741 4 GTCCTAC 1805 0.0 11.273354 1 CCCGTAC 95 1.4915629E-5 10.90953 16 GTACTAG 215 1.8189894E-12 10.463127 1 ACCGTAT 75 0.002838976 10.048903 8 TGTAGGA 2120 0.0 9.984165 2 ACAACCG 95 1.78906E-4 9.917038 5 GTCCTAA 1190 0.0 9.881619 1 TCCTACA 2025 0.0 9.846612 2 GGACGTG 2130 0.0 9.819265 6 GGTATCA 2095 0.0 9.76165 1 AGGACGT 2240 0.0 9.715598 5 GGCGAGG 940 0.0 9.622505 19 GTATAGC 395 0.0 9.5781555 1 GACGTGA 965 0.0 9.56763 7 GTCTTAC 225 6.184564E-11 9.543639 1 GTAGGAC 2190 0.0 9.464206 3 GTCCTAT 260 1.8189894E-12 9.438765 1 ACGGGTG 90 0.0012022557 9.421186 5 TATACTG 330 0.0 9.421186 5 >>END_MODULE