FastQCFastQC Report
Thu 26 May 2016
SRR1512174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2772250
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92650.3342050680854901No Hit
TATCAACGCAGAGTACTTTTTTTTT58070.20946884299756513No Hit
GGTATCAACGCAGAGTACTTTTTTT57730.20824240238073766No Hit
GTCCTACAGTGGACATTTCTAAATT41130.148363242853278No Hit
CTGTAGGACGTGGAATATGGCAAGA39920.1439985571286861No Hit
GTCCTAAAGTGTGTATTTCTCATTT34390.1240508612138155No Hit
CTTTAGGACGTGAAATATGGCGAGG32990.11900081161511408No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29580.10670033366399134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG500.001499281313.3009215
TAGGACC5900.012.2381364
AGGACGT23000.011.1942545
AGCGTAT600.005706284711.1274681
GGACGTG23050.011.0009346
GGTATCA23150.010.7944331
CCAACGA1252.2489075E-710.63766619
TTAGGAC13800.010.6021843
TAGGACG24650.010.5991124
GGCGAGG9650.010.53136819
GACGTGG11650.010.5160587
GTAGGAC20350.010.4577273
TGTAGGA21300.010.4373442
TCCAACG1553.6852725E-910.4161318
GACGTGA13050.010.18841657
TTACCGT750.002647167810.1340364
TGCGATA750.002655499610.13019810
ACCGCCT856.6152134E-410.0557118
GTCCTAC19750.09.8517321
CGTGGAA12200.09.8084399
Produced by FastQC (version 0.11.5)