FastQCFastQC Report
Thu 26 May 2016
SRR1512173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2929801
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74580.2545565381403037No Hit
GGTATCAACGCAGAGTACTTTTTTT45630.1557443662555921No Hit
TATCAACGCAGAGTACTTTTTTTTT44970.15349165352868677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAACG904.5340217E-511.3544262
CGCCTAC650.00619583911.0050591
TAGGACC5000.010.7411584
TTAGGAC9450.010.6683723
GGTATCA20800.010.51376151
GTCCTAC15100.010.2866591
GTCAACG1001.3216349E-410.2189842
GTATAGG3900.010.2189841
TCCAACG2400.010.20776318
GGCGAGG7850.010.08272819
TGGCGAG15500.09.90887518
TGTAGGA17450.09.8968942
CTAGTAC2002.910383E-119.8931713
GACGTGA7750.09.8475817
GTCCTAA8650.09.687361
CCCTATA1801.2872988E-89.6512622
CTTATAC4150.09.5355853
GTCTTAC2152.6193447E-109.5060321
GGACGTG15600.09.4824666
GTAGGAC16700.09.4784873
Produced by FastQC (version 0.11.5)