FastQCFastQC Report
Thu 26 May 2016
SRR1512173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2929801
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT102340.349307000714383No Hit
TATCAACGCAGAGTACTTTTTTTTT65340.22301855996362893No Hit
GGTATCAACGCAGAGTACTTTTTTT63060.21523646145250136No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32720.11167994003688304No Hit
GTCCTACAGTGGACATTTCTAAATT32340.1103829236183618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG508.7394015E-515.195669
TAAGACG551.9610091E-413.8182454
TGTCGAG1157.110066E-811.56191418
CGTGCGC1201.2817327E-711.08016810
GCGTGCG1053.4809182E-610.8540429
TAGGACG17200.010.7151654
AGGACGT16750.010.5493015
GTAGGAC15400.010.5487493
TAGGACC7400.010.5270744
GGACGTG16350.010.5138166
TCCAACG2350.010.50763618
GTCCTAC15350.010.5005591
GACGTGA9200.010.3231377
GGTATCA23800.010.2988861
AAGACCG1201.5248697E-610.2917135
TATGTCG1201.5305577E-610.28872716
TAGACTG4250.010.2823995
TGTAGGA16550.010.1601672
GACGTGG7950.010.1543327
TCCTACA17750.010.06201652
Produced by FastQC (version 0.11.5)