FastQCFastQC Report
Thu 26 May 2016
SRR1512172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2953175
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71930.24356836286369754No Hit
GTCCTACAGTGGACATTTCTAAATT65360.22132112048896527No Hit
CTGTAGGACGTGGAATATGGCAAGA57150.1935205329856849No Hit
GTCCTAAAGTGTGTATTTCTCATTT47030.1592523301192784No Hit
CTTTAGGACGTGAAATATGGCGAGG44330.150109627773498No Hit
GGTATCAACGCAGAGTACTTTTTTT43210.14631709939302615No Hit
TATCAACGCAGAGTACTTTTTTTTT41590.14083147798555792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT851.918852E-814.4082596
TCCAACG5000.013.00134418
TGGACCG802.1983215E-612.9535815
CCAACGA3400.012.74641619
TAGGACC10800.012.6482774
CGCTAGA751.04146646E-412.2758082
CGGTAGG1558.54925E-1111.8798141
ATCCCGT3500.011.84327410
GATATAC12100.011.3289141
GAACAGT9250.010.9994046
TTAGGAC20050.010.9948323
TACGCAG600.00625562510.9909165
CGCCCAA600.00625562510.99091610
GTCCTAC34250.010.961611
ACTGTTC11950.010.8792338
TGTAGGA31400.010.7511052
GGCGAGG14650.010.73959819
TGTAGAA14900.010.7104372
TTTAGGA19500.010.6495262
AGGACGT35050.010.6168625
Produced by FastQC (version 0.11.5)