FastQCFastQC Report
Thu 26 May 2016
SRR1512172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2953175
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92790.31420420394998605No Hit
GTCCTACAGTGGACATTTCTAAATT73840.25003597822682366No Hit
CTGTAGGACGTGGAATATGGCAAGA65120.22050843583600704No Hit
GGTATCAACGCAGAGTACTTTTTTT59410.2011733134677085No Hit
TATCAACGCAGAGTACTTTTTTTTT59000.19978497718557148No Hit
GTCCTAAAGTGTGTATTTCTCATTT53360.18068688784105244No Hit
CTTTAGGACGTGAAATATGGCGAGG52070.1763187078314018No Hit
TTGTAGAACAGTGTATATCAATGAG30200.10226281883058065No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30180.10219509510950078No Hit
GTCCTACAGTGTGCATTTCTCATTT29910.10128082487492276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG4850.014.09879418
CGGTCGC500.001503553813.29594710
TAGGACC10900.012.4635144
TGTCGAG1850.012.32056818
ATGTCGA1950.012.17577517
CCAACGA4000.012.10942519
CGGTTTC3150.012.05981513
TATGTCG2000.011.39652616
TGTAGAA15450.011.3755972
GGCGAGG14700.011.3066519
GACGTGG17600.011.115937
TGTAGGA35600.011.047942
GTAGGAC34950.011.0359533
GATATAC14350.010.9688591
GCCGGTT3250.010.81208911
CCGGTTT3450.010.73585712
CCAACGT1951.8189894E-1210.71522619
GGTATCA22000.010.710351
GGACGTG34750.010.6312916
CTGTAGG35450.010.5756171
Produced by FastQC (version 0.11.5)