FastQCFastQC Report
Thu 26 May 2016
SRR1512171_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512171_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139060
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47463.4129152883647347No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA41192.9620307780814037No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT35822.5758665324320438No Hit
GGTATCAACGCAGAGTACTTTTTTT32222.31698547389616No Hit
TATCAACGCAGAGTACTTTTTTTTT32072.3061987631238314No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15961.1477060261757515No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15701.1290090608370487No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12340.8873867395368905No Hit
GGTATCAACGCAGAGAAAAAAAAAA9270.6666187257299008No Hit
GGTATCAACGCAGAAAAAAAAAAAA8670.6234718826405867No Hit
GTACATAAGCAGTGGTATCAACGCA8280.5954264346325326No Hit
GTACTGGTTCACTATCGGTCAGTCA7910.5688192147274558No Hit
GTATCAACGCAGAGAAAAAAAAAAA6700.4818064144973393No Hit
ATACAGGGTGACAGCCCCGTACACA6510.4681432475190565No Hit
GGATACCACGTGTCCCGCCCTACTC5940.42715374658420824No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5860.42140083417229973No Hit
GTGGTATCAACGCAGAGTACATGGG5810.4178052639148569No Hit
GTGGTATCAACGCAGAGTACTTTTT5260.37825399108298574No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA5140.36962462246512295No Hit
GNATCAACGCAGAGTACTTTTTTTT4930.35452322738386305No Hit
ATCAACGCAGAGTACTTTTTTTTTT4610.33151157773622897No Hit
ATCATTAACTGAATCCATAGGTTAA4460.32072486696390046No Hit
GTATCAACGCAGAAAAAAAAAAAAA4130.2969941032647778No Hit
GGTATCAACGCAGAGTACATGGGGG4090.29411764705882354No Hit
TNTTTTTTTTTTTTTTTTTTTTTTT4060.29196030490435787No Hit
GCTTATGTACTCTGCGTTGATACCA3620.2603192866388609No Hit
TATCAACGCAGAGAAAAAAAAAAAA3450.2480943477635553No Hit
ACCCTGTATCGCGCGCCTTTCCAGA3420.2459370056090896No Hit
TNTCAACGCAGAGTACTTTTTTTTT3300.23730763699122678No Hit
GGTTAATGAGGCGAACCGGGGGAAC3270.23515029483676111No Hit
GNTATCAACGCAGAGTACTTTTTTT3170.22795915432187544No Hit
GTTAATGATAGTGTGTCGAAACACA3140.22580181216740974No Hit
GTATTTAGCCTTGGAGGATGGTCCC3110.22364447001294407No Hit
CCCATATTCAGACAGGATACCACGT3090.22220624190996693No Hit
ACTTAGATGTTTCAGTTCCCCCGGT3060.2200488997555012No Hit
GTACATGGGGGTTAAGCGACTAAGC3040.21861067165252407No Hit
GGTATCAACGCAGAGTACATGGGGA2970.21357687329210412No Hit
GCCCAGAGCCTGAATCAGTGTGTGT2970.21357687329210412No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA2960.2128577592406156No Hit
AGCGTACACGGTGGATGCCCTGGCA2950.21213864518912698No Hit
GTGGATGCCCTGGCAGTCAGAGGCG2930.21070041708614987No Hit
GTTCACTATCGGTCAGTCAGGAGTA2840.20422839062275278No Hit
GCCTTGGAGGATGGTCCCCCCATAT2830.20350927657126422No Hit
GTATCAACGCAGAGTACATGGGGGT2800.2013519344167985No Hit
TATTCAGACAGGATACCACGTGTCC2740.1970372501078671No Hit
TCTAAGTACCCCGAGGAAAAGAAAT2660.19128433769595857No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2580.18553142528405006No Hit
GAGTACATAAGCAGTGGTATCAACG2560.18409319718107292No Hit
GTTATAACCGGCGATTTCCGAATGG2560.18409319718107292No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG2550.18337408312958436No Hit
GTACTTAGATGTTTCAGTTCCCCCG2440.17546382856321013No Hit
CTTATGTACTCTGCGTTGATACCAC2400.17258737235725585No Hit
CATCTAAGTACCCCGAGGAAAAGAA2250.16180066158492737No Hit
GTGCTAATCTGCGATAAGCGTCGGT2240.16108154753343878No Hit
CTATCGGTCAGTCAGGAGTATTTAG2240.16108154753343878No Hit
GTCAGGAGTATTTAGCCTTGGAGGA2230.16036243348195023No Hit
TATGTACTCTGCGTTGATACCACTG2070.14885660865813316No Hit
GTACATGGGGGGTCTTGAGGGGTTT2070.14885660865813316No Hit
TATCAACGCAGAAAAAAAAAAAAAA2060.1481374946066446No Hit
GTACATGGGGAAAAAAAAAAAAAAA2000.1438228102977132No Hit
CACACACACTGATTCAGGCTCTGGG1980.1423845821947361No Hit
CAACGCAGAGTACTTTTTTTTTTTT1950.14022724004027037No Hit
ACGCAGAGTACATGGGGGTTAAGCG1930.13878901193729323No Hit
GGTTCACTATCGGTCAGTCAGGAGT1910.13735078383431612No Hit
TCACGGTACTGGTTCACTATCGGTC1900.13663166978282756No Hit
GCCTGAATCAGTGTGTGTGTTAGTG1840.13231698547389617No Hit
GNACTTTTTTTTTTTTTTTTTTTTT1830.1315978714224076No Hit
GATATGAACCGTTATAACCGGCGAT1830.1315978714224076No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1800.1294405292679419No Hit
GAATCAGTGTGTGTGTTAGTGGAAG1800.1294405292679419No Hit
GTATCAACGCAGAGTACATGGGGAA1800.1294405292679419No Hit
TCATTAACCTATGGATTCAGTTAAT1780.12800230116496475No Hit
GGTGATATGAACCGTTATAACCGGC1750.12584495901049908No Hit
CATAAGCAGTGGTATCAACGCAGAG1740.1251258449590105No Hit
CGGTACTGGTTCACTATCGGTCAGT1700.12224938875305623No Hit
GTCCCGCCCTACTCATCGAGCTCAC1690.12153027470156767No Hit
CACTATCATTAACTGAATCCATAGG1690.12153027470156767No Hit
AACGCAGAGTACTTTTTTTTTTTTT1670.12009204659859053No Hit
GGGTGACAGCCCCGTACACAAAAAT1670.12009204659859053No Hit
GTGGTATCCTGTCTGAATATGGGGG1650.11865381849561339No Hit
GTATCAACGCAGAGTACATGGGGGG1630.11721559039263628No Hit
GGGTACTTAGATGTTTCAGTTCCCC1630.11721559039263628No Hit
GTGGTATCAACGCAGAAAAAAAAAA1610.11577736228965915No Hit
ANGCAGAGTACTTTTTTTTTTTTTT1610.11577736228965915No Hit
GCCCTACTCATCGAGCTCACAGCAT1600.11505824823817058No Hit
GGTATCAACGCAGAGTACATGGGAA1600.11505824823817058No Hit
AGCTCACAGCATGTGCATTTTTGTG1590.11433913418668201No Hit
GTACATGGGAAAAAAAAAAAAAAAA1570.11290090608370487No Hit
TCAACGCAGAGTACTTTTTTTTTTT1560.11218179203221632No Hit
CCGTTATAACCGGCGATTTCCGAAT1540.11074356392923917No Hit
ATCCATAGGTTAATGAGGCGAACCG1530.1100244498777506No Hit
TTCCAGACGCTTCCACTAACACACA1530.1100244498777506No Hit
CCCCAGTAGCGGCGAGCGAACGGGG1510.10858622177477348No Hit
ACGCAGAGTACATAAGCAGTGGTAT1510.10858622177477348No Hit
GGAGTATTTAGCCTTGGAGGATGGT1500.10786710772328491No Hit
ACGCAGAGAAAAAAAAAAAAAAAAA1480.1064288796203078No Hit
CTATCATTAACTGAATCCATAGGTT1480.1064288796203078No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG1470.10570976556881923No Hit
CTGTGAGCTCGATGAGTAGGGCGGG1450.10427153746584208No Hit
GGGTTTCCCCATTCGGAAATCGCCG1440.10355242341435351No Hit
CCCATGTACTCTGCGTTGATACCAC1430.10283330936286496No Hit
TCGGTAAGGTGATATGAACCGTTAT1400.10067596720839925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCAA350.00126137917.6431942
GTTATCA350.00126137917.6431941
GGGTCTT400.005570379614.12216810
GGGGTCT400.005570379614.1221689
CCCCCGG751.5971007E-512.55303918
CCCCGGT701.1717087E-412.10471519
GACTAAG550.003256863711.98244618
CGGGGGA1501.6370905E-1111.92538617
TGTCCCG1302.901288E-911.5874211
GGTATCA12050.011.5303031
GGGGAAC1800.011.50695219
CGCCTTT752.2268025E-411.29773414
ACCGGGG1605.0931703E-1111.18004915
AGGCGAA1605.0931703E-1111.1800499
CCGGGGG1605.0931703E-1111.18004916
GTGTCCC1355.2423275E-911.15825710
GCGAACC1701.2732926E-1111.07621111
AGGGTGA951.4758469E-510.9013235
GAACCGG1658.913048E-1110.84126113
GCCTTTC804.0476973E-410.59162515
Produced by FastQC (version 0.11.5)