##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512171_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 139060 Sequences flagged as poor quality 0 Sequence length 25 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.068977419818783 31.0 30.0 33.0 25.0 34.0 2 25.632432043722133 31.0 25.0 33.0 2.0 34.0 3 27.375809003307925 31.0 25.0 33.0 16.0 34.0 4 32.15344455630663 35.0 32.0 37.0 22.0 37.0 5 32.72905220768014 35.0 33.0 37.0 26.0 37.0 6 33.10217172443549 35.0 35.0 37.0 27.0 37.0 7 33.147152308356105 36.0 35.0 37.0 27.0 37.0 8 33.19253559614555 37.0 35.0 37.0 27.0 37.0 9 34.52005609089601 39.0 35.0 39.0 27.0 39.0 10 34.460916151301596 38.0 35.0 39.0 27.0 39.0 11 34.445735653674674 38.0 35.0 39.0 27.0 39.0 12 34.29959729613117 38.0 35.0 39.0 27.0 39.0 13 34.209233424421114 38.0 35.0 39.0 26.0 39.0 14 35.006191571983315 39.0 34.0 41.0 25.0 41.0 15 34.80409895009348 39.0 34.0 41.0 25.0 41.0 16 34.78169854738962 39.0 34.0 41.0 25.0 41.0 17 34.98484107579462 39.0 35.0 40.0 26.0 41.0 18 34.88710628505681 38.0 35.0 40.0 25.0 41.0 19 34.9102905220768 39.0 35.0 41.0 25.0 41.0 20 34.887336401553284 39.0 35.0 41.0 25.0 41.0 21 34.84149288077089 39.0 35.0 41.0 25.0 41.0 22 34.68392780094923 39.0 35.0 41.0 25.0 41.0 23 34.46214583632964 38.0 34.0 41.0 23.0 41.0 24 34.28364734646915 38.0 34.0 41.0 22.0 41.0 25 34.094807996548255 38.0 34.0 40.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.7284885707730773 1101 2 2.7102373580621446 1101 3 1.1890594943771617 1101 4 -0.8367745934492667 1101 5 -0.29656581921668845 1101 6 -0.4593286022511265 1101 7 -0.6456593726694919 1101 8 -0.7802540083474412 1101 9 -1.2052442994552948 1101 10 -0.5149698066008028 1101 11 -0.7414612687810518 1101 12 -0.41585628734770097 1101 13 -0.6971066909577175 1101 14 -1.0351964547760204 1101 15 -0.6898806750101798 1101 16 -0.3107082165614088 1101 17 -0.7338404010131825 1101 18 -0.09506536036359137 1101 19 -0.6949973173736197 1101 20 -0.8626120831945059 1101 21 -1.5431286243319065 1101 22 -1.1039649264022344 1101 23 -1.282844526949738 1101 24 -0.9046117095256534 1101 25 -0.6705193555656663 1102 1 0.8365216402548334 1102 2 3.7909895704515257 1102 3 2.4652677993465275 1102 4 1.3815712200292936 1102 5 0.7105819003204061 1102 6 1.1292520648693625 1102 7 1.4338164612311246 1102 8 1.095256543048066 1102 9 0.22429960796471704 1102 10 0.5039205950329588 1102 11 0.4635251165150649 1102 12 0.15587618372105538 1102 13 -0.3640569972885572 1102 14 0.3038096718407246 1102 15 -1.0814735953641588 1102 16 -0.08734198102703061 1102 17 0.06313032737347157 1102 18 0.5432089759195975 1102 19 0.11082296853516027 1102 20 0.3663702176904522 1102 21 0.5089476180098202 1102 22 0.5349901450749641 1102 23 0.41022899245121636 1102 24 0.7873556151850352 1102 25 0.7359646471572745 1103 1 1.1973603550758511 1103 2 4.213615736440524 1103 3 2.548900104217772 1103 4 1.4121859055112296 1103 5 1.0003849777341074 1103 6 0.9096076416851204 1103 7 0.6885402115300963 1103 8 0.8308859777925406 1103 9 0.32905923484823774 1103 10 0.4249123832813879 1103 11 0.08450893218914501 1103 12 0.4802802337888181 1103 13 0.9229556791046534 1103 14 0.9058984863189181 1103 15 0.6817409466114412 1103 16 1.088373557520363 1103 17 1.1734707562979736 1103 18 1.0569616666634332 1103 19 1.3204219480456416 1103 20 1.453829358246935 1103 21 1.2638706826674024 1103 22 0.8463988989615956 1103 23 1.2749344058291996 1103 24 0.789428484514545 1103 25 0.8595395494932347 1104 1 1.0536704466800586 1104 2 4.314228954700798 1104 3 2.5085488285723763 1104 4 1.5900192009915841 1104 5 1.4878556416753597 1104 6 1.3405259549563695 1104 7 0.8890852944281207 1104 8 1.10395736851099 1104 9 1.2588798117276383 1104 10 1.288036011110897 1104 11 1.0535451953430552 1104 12 1.0548127494978061 1104 13 0.5470755973422143 1104 14 0.7618300481328291 1104 15 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-0.8692586542116061 2315 13 -1.3938522530878927 2315 14 -1.2955514843618232 2315 15 -1.3126617400989389 2315 16 -0.8158857313543066 2315 17 -0.7944912885871531 2315 18 -0.8398878455706935 2315 19 -1.1639322286162255 2315 20 -1.3248901897033818 2315 21 -1.4277440089472933 2315 22 -1.0677223228519352 2315 23 -0.9714539944053584 2315 24 -0.7655584550877776 2315 25 -0.7496613673999875 2316 1 -0.7486862501886336 2316 2 0.3324440619727582 2316 3 0.005239124802599804 2316 4 -0.5186611426542491 2316 5 -0.3260028626202143 2316 6 -0.20282881712005718 2316 7 -0.6391058703059329 2316 8 -0.7908363031476142 2316 9 -0.3608094047410759 2316 10 -0.7005091276149855 2316 11 -1.4061045863573298 2316 12 -0.8401687067718484 2316 13 -0.10890299521211944 2316 14 -0.4417284702465878 2316 15 -1.336528719702919 2316 16 -0.5658113536477885 2316 17 -0.9155120812882203 2316 18 -1.0203446899725321 2316 19 -0.6978980479279855 2316 20 -0.6244835915743963 2316 21 -0.12950593417290435 2316 22 -0.48562586323076573 2316 23 -0.36304435505459054 2316 24 -0.22154338120592598 2316 25 -0.3850122648909817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5583.0 3 0.0 4 33.0 5 182.0 6 239.0 7 256.0 8 206.0 9 233.0 10 226.0 11 213.0 12 201.0 13 222.0 14 217.0 15 204.0 16 245.0 17 279.0 18 288.0 19 337.0 20 441.0 21 694.0 22 775.0 23 934.0 24 1066.0 25 1310.0 26 1455.0 27 1727.0 28 1954.0 29 2349.0 30 3140.0 31 4017.0 32 5143.0 33 6899.0 34 9553.0 35 13125.0 36 19794.0 37 22631.0 38 32889.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.08845102833309 19.78929958291385 15.394074500215735 14.728174888537321 2 18.29145491448621 24.58973834510754 38.404109696807595 18.71469704359866 3 20.78239608801956 32.29469293830001 30.164677117790884 16.758233855889543 4 11.717963469006184 21.73522220624191 34.78282755645045 31.76398676830145 5 10.637854163670358 40.778800517762114 32.03940745002157 16.543937868545953 6 24.174457068891126 37.45217891557601 20.34445563066302 18.02890838486984 7 21.69388650370722 40.13146066607696 20.99271500794661 17.181937822269205 8 25.8614986336833 31.763267654249965 21.94664173737955 20.428591974687187 9 28.281317416942326 16.54969078095786 21.317416942327053 33.85157485977276 10 21.57948481928404 29.159775057889515 25.5598383408362 23.70090178199025 11 29.114260853306867 28.84315290632034 20.40141235015353 21.64117389021926 12 25.72199050769452 31.967496044872718 26.316697828275565 15.993815619157198 13 30.78814900043147 25.63569682151589 27.583057672946932 15.993096505105711 14 23.730763699122683 30.227240040270388 28.55529987055947 17.486696390047463 15 26.330360995253848 29.19890694664174 29.36717963469006 15.103552423414355 16 17.2012081116065 34.27585215015102 28.480512009204663 20.042427729037826 17 17.324176614411048 32.96922191859629 28.10944915863656 21.597152308356105 18 15.901359932686567 28.129247542268665 36.589452790702694 19.379939734342074 19 17.497986422736165 31.175209987343226 35.936601081578644 15.390202508341963 20 19.14641162088307 27.035811879764132 37.52480943477636 16.29296706457644 21 20.726480360147857 29.48710572870971 35.06695240697859 14.71946150416385 22 19.472590376608153 29.46417656069094 34.82025356508482 16.242979497616087 23 19.83071690026392 28.852916429953186 35.54873181501111 15.767634854771783 24 18.451315022977845 29.813660129308794 34.65015426438542 17.08487058332794 25 18.03053511970256 31.37508719696806 36.01648292377727 14.577894759552112 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8917.0 1 8917.0 2 5371.0 3 1825.0 4 1825.0 5 1825.0 6 2162.5 7 2500.0 8 2500.0 9 2500.0 10 2184.5 11 1869.0 12 1869.0 13 1869.0 14 1341.5 15 814.0 16 814.0 17 814.0 18 1622.0 19 2430.0 20 2430.0 21 2430.0 22 3704.0 23 4978.0 24 4978.0 25 4978.0 26 7435.5 27 9893.0 28 9893.0 29 9893.0 30 10737.5 31 11582.0 32 11582.0 33 11582.0 34 10431.5 35 9281.0 36 9281.0 37 9281.0 38 8231.0 39 7181.0 40 7181.0 41 7181.0 42 10067.5 43 12954.0 44 12954.0 45 12954.0 46 15022.5 47 17091.0 48 17091.0 49 17091.0 50 16550.5 51 16010.0 52 16010.0 53 16010.0 54 13990.0 55 11970.0 56 11970.0 57 11970.0 58 9822.5 59 7675.0 60 7675.0 61 7675.0 62 7207.0 63 6739.0 64 6739.0 65 6739.0 66 4773.5 67 2808.0 68 2808.0 69 2808.0 70 2228.0 71 1648.0 72 1648.0 73 1648.0 74 1154.5 75 661.0 76 661.0 77 661.0 78 420.5 79 180.0 80 180.0 81 180.0 82 109.5 83 39.0 84 39.0 85 39.0 86 26.0 87 13.0 88 13.0 89 13.0 90 7.5 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 8.590536459082411 3 0.0 4 0.0 5 0.0 6 0.0 7 0.005033798360419962 8 0.0 9 0.0 10 0.0014382281029771322 11 7.191140514885661E-4 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.006472026463397095 19 0.0028764562059542644 20 0.0 21 0.004314684308931396 22 0.002157342154465698 23 0.002157342154465698 24 0.009348482669351359 25 0.005033798360419962 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 139060.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.60002876456206 #Duplication Level Percentage of deduplicated Percentage of total 1 81.8560407801015 25.86653243204372 2 9.143663382108642 5.778800517762117 3 2.8832806135220626 2.73335250970804 4 1.3699565346016431 1.7316266359844672 5 0.7395944746603554 1.1685603336689199 6 0.5689188266618118 1.0786710772328492 7 0.4096215551965046 0.9060837048755932 8 0.2799080627176115 0.7076082266647491 9 0.23211888127801927 0.6601466992665036 >10 1.8660537514507431 12.421257011362002 >50 0.3163188676239674 7.050194160793902 >100 0.2912864392508477 17.740543650222925 >500 0.025032428373119725 5.418524377966345 >1k 0.01820540245317798 16.738098662447864 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4746 3.4129152883647347 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 4119 2.9620307780814037 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 3582 2.5758665324320438 No Hit GGTATCAACGCAGAGTACTTTTTTT 3222 2.31698547389616 No Hit TATCAACGCAGAGTACTTTTTTTTT 3207 2.3061987631238314 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1596 1.1477060261757515 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1570 1.1290090608370487 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1234 0.8873867395368905 No Hit GGTATCAACGCAGAGAAAAAAAAAA 927 0.6666187257299008 No Hit GGTATCAACGCAGAAAAAAAAAAAA 867 0.6234718826405867 No Hit GTACATAAGCAGTGGTATCAACGCA 828 0.5954264346325326 No Hit GTACTGGTTCACTATCGGTCAGTCA 791 0.5688192147274558 No Hit GTATCAACGCAGAGAAAAAAAAAAA 670 0.4818064144973393 No Hit ATACAGGGTGACAGCCCCGTACACA 651 0.4681432475190565 No Hit GGATACCACGTGTCCCGCCCTACTC 594 0.42715374658420824 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 586 0.42140083417229973 No Hit GTGGTATCAACGCAGAGTACATGGG 581 0.4178052639148569 No Hit GTGGTATCAACGCAGAGTACTTTTT 526 0.37825399108298574 No Hit ANAAAAAAAAAAAAAAAAAAAAAAA 514 0.36962462246512295 No Hit GNATCAACGCAGAGTACTTTTTTTT 493 0.35452322738386305 No Hit ATCAACGCAGAGTACTTTTTTTTTT 461 0.33151157773622897 No Hit ATCATTAACTGAATCCATAGGTTAA 446 0.32072486696390046 No Hit GTATCAACGCAGAAAAAAAAAAAAA 413 0.2969941032647778 No Hit GGTATCAACGCAGAGTACATGGGGG 409 0.29411764705882354 No Hit TNTTTTTTTTTTTTTTTTTTTTTTT 406 0.29196030490435787 No Hit GCTTATGTACTCTGCGTTGATACCA 362 0.2603192866388609 No Hit TATCAACGCAGAGAAAAAAAAAAAA 345 0.2480943477635553 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 342 0.2459370056090896 No Hit TNTCAACGCAGAGTACTTTTTTTTT 330 0.23730763699122678 No Hit GGTTAATGAGGCGAACCGGGGGAAC 327 0.23515029483676111 No Hit GNTATCAACGCAGAGTACTTTTTTT 317 0.22795915432187544 No Hit GTTAATGATAGTGTGTCGAAACACA 314 0.22580181216740974 No Hit GTATTTAGCCTTGGAGGATGGTCCC 311 0.22364447001294407 No Hit CCCATATTCAGACAGGATACCACGT 309 0.22220624190996693 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 306 0.2200488997555012 No Hit GTACATGGGGGTTAAGCGACTAAGC 304 0.21861067165252407 No Hit GGTATCAACGCAGAGTACATGGGGA 297 0.21357687329210412 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 297 0.21357687329210412 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 296 0.2128577592406156 No Hit AGCGTACACGGTGGATGCCCTGGCA 295 0.21213864518912698 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 293 0.21070041708614987 No Hit GTTCACTATCGGTCAGTCAGGAGTA 284 0.20422839062275278 No Hit GCCTTGGAGGATGGTCCCCCCATAT 283 0.20350927657126422 No Hit GTATCAACGCAGAGTACATGGGGGT 280 0.2013519344167985 No Hit TATTCAGACAGGATACCACGTGTCC 274 0.1970372501078671 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 266 0.19128433769595857 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 258 0.18553142528405006 No Hit GAGTACATAAGCAGTGGTATCAACG 256 0.18409319718107292 No Hit GTTATAACCGGCGATTTCCGAATGG 256 0.18409319718107292 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 255 0.18337408312958436 No Hit GTACTTAGATGTTTCAGTTCCCCCG 244 0.17546382856321013 No Hit CTTATGTACTCTGCGTTGATACCAC 240 0.17258737235725585 No Hit CATCTAAGTACCCCGAGGAAAAGAA 225 0.16180066158492737 No Hit GTGCTAATCTGCGATAAGCGTCGGT 224 0.16108154753343878 No Hit CTATCGGTCAGTCAGGAGTATTTAG 224 0.16108154753343878 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 223 0.16036243348195023 No Hit TATGTACTCTGCGTTGATACCACTG 207 0.14885660865813316 No Hit GTACATGGGGGGTCTTGAGGGGTTT 207 0.14885660865813316 No Hit TATCAACGCAGAAAAAAAAAAAAAA 206 0.1481374946066446 No Hit GTACATGGGGAAAAAAAAAAAAAAA 200 0.1438228102977132 No Hit CACACACACTGATTCAGGCTCTGGG 198 0.1423845821947361 No Hit CAACGCAGAGTACTTTTTTTTTTTT 195 0.14022724004027037 No Hit ACGCAGAGTACATGGGGGTTAAGCG 193 0.13878901193729323 No Hit GGTTCACTATCGGTCAGTCAGGAGT 191 0.13735078383431612 No Hit TCACGGTACTGGTTCACTATCGGTC 190 0.13663166978282756 No Hit GCCTGAATCAGTGTGTGTGTTAGTG 184 0.13231698547389617 No Hit GNACTTTTTTTTTTTTTTTTTTTTT 183 0.1315978714224076 No Hit GATATGAACCGTTATAACCGGCGAT 183 0.1315978714224076 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 180 0.1294405292679419 No Hit GAATCAGTGTGTGTGTTAGTGGAAG 180 0.1294405292679419 No Hit GTATCAACGCAGAGTACATGGGGAA 180 0.1294405292679419 No Hit TCATTAACCTATGGATTCAGTTAAT 178 0.12800230116496475 No Hit GGTGATATGAACCGTTATAACCGGC 175 0.12584495901049908 No Hit CATAAGCAGTGGTATCAACGCAGAG 174 0.1251258449590105 No Hit CGGTACTGGTTCACTATCGGTCAGT 170 0.12224938875305623 No Hit GTCCCGCCCTACTCATCGAGCTCAC 169 0.12153027470156767 No Hit CACTATCATTAACTGAATCCATAGG 169 0.12153027470156767 No Hit AACGCAGAGTACTTTTTTTTTTTTT 167 0.12009204659859053 No Hit GGGTGACAGCCCCGTACACAAAAAT 167 0.12009204659859053 No Hit GTGGTATCCTGTCTGAATATGGGGG 165 0.11865381849561339 No Hit GTATCAACGCAGAGTACATGGGGGG 163 0.11721559039263628 No Hit GGGTACTTAGATGTTTCAGTTCCCC 163 0.11721559039263628 No Hit GTGGTATCAACGCAGAAAAAAAAAA 161 0.11577736228965915 No Hit ANGCAGAGTACTTTTTTTTTTTTTT 161 0.11577736228965915 No Hit GCCCTACTCATCGAGCTCACAGCAT 160 0.11505824823817058 No Hit GGTATCAACGCAGAGTACATGGGAA 160 0.11505824823817058 No Hit AGCTCACAGCATGTGCATTTTTGTG 159 0.11433913418668201 No Hit GTACATGGGAAAAAAAAAAAAAAAA 157 0.11290090608370487 No Hit TCAACGCAGAGTACTTTTTTTTTTT 156 0.11218179203221632 No Hit CCGTTATAACCGGCGATTTCCGAAT 154 0.11074356392923917 No Hit ATCCATAGGTTAATGAGGCGAACCG 153 0.1100244498777506 No Hit TTCCAGACGCTTCCACTAACACACA 153 0.1100244498777506 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 151 0.10858622177477348 No Hit ACGCAGAGTACATAAGCAGTGGTAT 151 0.10858622177477348 No Hit GGAGTATTTAGCCTTGGAGGATGGT 150 0.10786710772328491 No Hit ACGCAGAGAAAAAAAAAAAAAAAAA 148 0.1064288796203078 No Hit CTATCATTAACTGAATCCATAGGTT 148 0.1064288796203078 No Hit GTGTTAGTGGAAGCGTCTGGAAAGG 147 0.10570976556881923 No Hit CTGTGAGCTCGATGAGTAGGGCGGG 145 0.10427153746584208 No Hit GGGTTTCCCCATTCGGAAATCGCCG 144 0.10355242341435351 No Hit CCCATGTACTCTGCGTTGATACCAC 143 0.10283330936286496 No Hit TCGGTAAGGTGATATGAACCGTTAT 140 0.10067596720839925 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATCAA 35 0.001261379 17.643194 2 GTTATCA 35 0.001261379 17.643194 1 GGGTCTT 40 0.0055703796 14.122168 10 GGGGTCT 40 0.0055703796 14.122168 9 CCCCCGG 75 1.5971007E-5 12.553039 18 CCCCGGT 70 1.1717087E-4 12.104715 19 GACTAAG 55 0.0032568637 11.982446 18 CGGGGGA 150 1.6370905E-11 11.925386 17 TGTCCCG 130 2.901288E-9 11.58742 11 GGTATCA 1205 0.0 11.530303 1 GGGGAAC 180 0.0 11.506952 19 CGCCTTT 75 2.2268025E-4 11.297734 14 ACCGGGG 160 5.0931703E-11 11.180049 15 AGGCGAA 160 5.0931703E-11 11.180049 9 CCGGGGG 160 5.0931703E-11 11.180049 16 GTGTCCC 135 5.2423275E-9 11.158257 10 GCGAACC 170 1.2732926E-11 11.076211 11 AGGGTGA 95 1.4758469E-5 10.901323 5 GAACCGG 165 8.913048E-11 10.841261 13 GCCTTTC 80 4.0476973E-4 10.591625 15 >>END_MODULE