FastQCFastQC Report
Thu 26 May 2016
SRR1512171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139060
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64324.625341579174457No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT51003.6674816625916873No Hit
GGTATCAACGCAGAGTACTTTTTTT46933.3748022436358402No Hit
TATCAACGCAGAGTACTTTTTTTTT46203.3223069178771754No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA44233.180641449733928No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26281.8898317273119518No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21161.5216453329498059No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15051.082266647490292No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8220.5911117503236013No Hit
GTACATAAGCAGTGGTATCAACGCA8160.58679706601467No Hit
GTGGTATCAACGCAGAGTACTTTTT8000.5752912411908528No Hit
GGTATCAACGCAGAAAAAAAAAAAA7880.56666187257299No Hit
GTACTGGTTCACTATCGGTCAGTCA7740.5565942758521502No Hit
GGTATCAACGCAGAGAAAAAAAAAA7560.543650222925356No Hit
ATACAGGGTGACAGCCCCGTACACA7190.5170430030202791No Hit
ATCAACGCAGAGTACTTTTTTTTTT6880.4947504674241335No Hit
GTGGTATCAACGCAGAGTACATGGG6850.49259312526966775No Hit
GGATACCACGTGTCCCGCCCTACTC6550.4710197037250108No Hit
GTATCAACGCAGAGAAAAAAAAAAA5710.4106141233999713No Hit
ATCATTAACTGAATCCATAGGTTAA5440.3911980440097799No Hit
GGTATCAACGCAGAGTACATGGGGG4350.3128146123975262No Hit
AGCGTACACGGTGGATGCCCTGGCA4290.30849992808859483No Hit
GGTTAATGAGGCGAACCGGGGGAAC4180.3005896735222206No Hit
TATCAACGCAGAGAAAAAAAAAAAA3870.2782971379260751No Hit
GCTTATGTACTCTGCGTTGATACCA3840.27613979577160935No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA3800.27326333956565513No Hit
ACCCTGTATCGCGCGCCTTTCCAGA3750.2696677693082123No Hit
ACTTAGATGTTTCAGTTCCCCCGGT3700.26607219905076945No Hit
GTTAATGATAGTGTGTCGAAACACA3650.2624766287933266No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3620.2603192866388609No Hit
GTGGATGCCCTGGCAGTCAGAGGCG3470.2495325758665324No Hit
TCTAAGTACCCCGAGGAAAAGAAAT3390.24377966345462393No Hit
GTACATGGGGGTTAAGCGACTAAGC3240.23299295268229542No Hit
GTATCAACGCAGAAAAAAAAAAAAA3240.23299295268229542No Hit
GTATTTAGCCTTGGAGGATGGTCCC3150.2265209262188983No Hit
GTATCAACGCAGAGTACATGGGGGT3140.22580181216740974No Hit
CCCATATTCAGACAGGATACCACGT3110.22364447001294407No Hit
CAACGCAGAGTACTTTTTTTTTTTT2950.21213864518912698No Hit
TATTCAGACAGGATACCACGTGTCC2860.20566661872572992No Hit
GTTATAACCGGCGATTTCCGAATGG2820.20279016251977564No Hit
GGTATCAACGCAGAGTACATGGGGA2780.19991370631382135No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2770.19919459226233283No Hit
AACGCAGAGTACTTTTTTTTTTTTT2760.19847547821084421No Hit
GCCTTGGAGGATGGTCCCCCCATAT2750.19775636415935569No Hit
GCCCAGAGCCTGAATCAGTGTGTGT2690.19344167985042426No Hit
CTTATGTACTCTGCGTTGATACCAC2670.19200345174744715No Hit
GAGTACATAAGCAGTGGTATCAACG2660.19128433769595857No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG2630.18912699554149287No Hit
GTCAGGAGTATTTAGCCTTGGAGGA2600.1869696533870272No Hit
TATGTACTCTGCGTTGATACCACTG2590.18625053933553862No Hit
GTTCACTATCGGTCAGTCAGGAGTA2550.18337408312958436No Hit
CATCTAAGTACCCCGAGGAAAAGAA2470.17762117071767583No Hit
GTACTTAGATGTTTCAGTTCCCCCG2370.17043003020279016No Hit
CACACACACTGATTCAGGCTCTGGG2370.17043003020279016No Hit
TCATTAACCTATGGATTCAGTTAAT2360.1697109161513016No Hit
ACGCAGAGTACATGGGGGTTAAGCG2320.16683445994534735No Hit
TCAACGCAGAGTACTTTTTTTTTTT2290.16467711779088165No Hit
CGGTACTGGTTCACTATCGGTCAGT2250.16180066158492737No Hit
GTGCTAATCTGCGATAAGCGTCGGT2180.15676686322450742No Hit
CACTATCATTAACTGAATCCATAGG2180.15676686322450742No Hit
TCACGGTACTGGTTCACTATCGGTC2130.15317129296706458No Hit
CTATCGGTCAGTCAGGAGTATTTAG2080.14957572270962174No Hit
TCGGTAAGGTGATATGAACCGTTAT2040.1466992665036675No Hit
GATATGAACCGTTATAACCGGCGAT2010.1445419243492018No Hit
GAATCAGTGTGTGTGTTAGTGGAAG1980.1423845821947361No Hit
AGCTCACAGCATGTGCATTTTTGTG1960.14094635409175896No Hit
GTGGTATCCTGTCTGAATATGGGGG1930.13878901193729323No Hit
ATCCATAGGTTAATGAGGCGAACCG1890.135912555731339No Hit
CATAAGCAGTGGTATCAACGCAGAG1840.13231698547389617No Hit
GGTGATATGAACCGTTATAACCGGC1840.13231698547389617No Hit
GGGTACTTAGATGTTTCAGTTCCCC1800.1294405292679419No Hit
TATCAACGCAGAGTACATGGGGGTT1740.1251258449590105No Hit
CTATCATTAACTGAATCCATAGGTT1710.1229685028045448No Hit
GTACATGGGGAAAAAAAAAAAAAAA1700.12224938875305623No Hit
GGAGTATTTAGCCTTGGAGGATGGT1690.12153027470156767No Hit
GCCTGAATCAGTGTGTGTGTTAGTG1680.1208111606500791No Hit
GTATCAACGCAGAGTACATGGGGAA1680.1208111606500791No Hit
CCTATGGATTCAGTTAATGATAGTG1670.12009204659859053No Hit
CCCCAGTAGCGGCGAGCGAACGGGG1640.11793470444412482No Hit
GGTTCACTATCGGTCAGTCAGGAGT1640.11793470444412482No Hit
ACGCAGAGTACATAAGCAGTGGTAT1620.11649647634114771No Hit
TATCAACGCAGAAAAAAAAAAAAAA1560.11218179203221632No Hit
ACGCAGAGAAAAAAAAAAAAAAAAA1550.11146267798072775No Hit
GGTATCAACGCAGAGTACATGGGAA1540.11074356392923917No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG1530.1100244498777506No Hit
TTCCAGACGCTTCCACTAACACACA1520.10930533582626203No Hit
GGGTGACAGCCCCGTACACAAAAAT1520.10930533582626203No Hit
GAGTACATGGGGGTTAAGCGACTAA1510.10858622177477348No Hit
GCGTACACGGTGGATGCCCTGGCAG1490.10714799367179635No Hit
GTACATGGGGGGTCTTGAGGGGTTT1480.1064288796203078No Hit
CAGTAGCGGCGAGCGAACGGGGAGC1460.10499065151733065No Hit
GGGTTTCCCCATTCGGAAATCGCCG1410.10139508125988782No Hit
ATGATAGTGTGTCGAAACACACTGG1410.10139508125988782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGAT359.880025E-518.99424617
GTTGATT402.7448367E-416.61996718
GCGCGCG402.7448367E-416.61996711
CGCGCGA402.7448367E-416.61996712
GTGTGTC704.3671935E-714.9240511
GGCGCGC456.723139E-414.77330310
TCGGTTG500.001491006213.29597315
TTGATTT500.001491006213.29597319
CGGTTGA500.001491006213.29597316
GTGTCGA801.9690433E-613.05854513
TAGTGTG801.9690433E-613.0585459
TGTCGAA801.9690433E-613.05854514
CCTACTC1301.9099389E-1012.41931519
CCCTACT1301.9099389E-1012.41931518
CGCCCTA1155.169568E-912.38755316
CCGCCCT1103.718924E-812.08724815
AGGCGCG550.003051272812.0872489
TCTCGGT550.003051272812.08724813
ATGATAG907.3201263E-611.6117715
TAATGAT907.3201263E-611.6117713
Produced by FastQC (version 0.11.5)