##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512171_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 139060 Sequences flagged as poor quality 0 Sequence length 25 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.10770890263196 31.0 30.0 34.0 26.0 34.0 2 30.175456637422695 31.0 30.0 34.0 26.0 34.0 3 30.10635696821516 31.0 30.0 34.0 26.0 34.0 4 33.89375089889256 35.0 35.0 37.0 30.0 37.0 5 33.437070329354235 35.0 35.0 37.0 28.0 37.0 6 33.44021285775924 36.0 35.0 37.0 28.0 37.0 7 33.2262332805983 36.0 35.0 37.0 27.0 37.0 8 33.15430749316842 36.0 35.0 37.0 27.0 37.0 9 34.385315691068605 38.0 34.0 39.0 27.0 39.0 10 34.24983460376816 38.0 34.0 39.0 26.0 39.0 11 34.1955558751618 38.0 34.0 39.0 26.0 39.0 12 33.93412196174313 37.0 34.0 39.0 25.0 39.0 13 33.833345318567524 37.0 33.0 39.0 25.0 39.0 14 34.434790737811014 38.0 33.0 40.0 23.0 41.0 15 34.25762260894578 38.0 33.0 40.0 22.0 41.0 16 34.126549690780955 38.0 33.0 40.0 20.0 41.0 17 34.30570976556882 38.0 33.0 40.0 24.0 41.0 18 34.21162807421257 38.0 33.0 40.0 23.0 41.0 19 34.31099525384726 38.0 34.0 40.0 24.0 41.0 20 34.28010930533583 38.0 34.0 40.0 23.0 41.0 21 34.168330217172446 38.0 34.0 40.0 22.0 41.0 22 34.03411477060262 38.0 34.0 40.0 21.0 41.0 23 33.7218826405868 38.0 33.0 40.0 18.0 41.0 24 33.515978714224076 38.0 33.0 40.0 17.0 41.0 25 33.174097511865384 37.0 33.0 40.0 15.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1687888053314026 1101 2 -0.047436162899710865 1101 3 -0.10599782908724009 1101 4 -0.4057022163171595 1101 5 -0.3545915508545363 1101 6 -0.37128773630615086 1101 7 -0.08137422585761556 1101 8 -0.923858712444023 1101 9 -0.3232325619091725 1101 10 -0.30734986796740316 1101 11 -0.30891813141472113 1101 12 -0.2816533659930158 1101 13 -0.4467713295511473 1101 14 -0.9809017163202398 1101 15 -0.203463797305524 1101 16 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0.4287419382783888 1103 3 0.7546224065329952 1103 4 0.8035839929690454 1103 5 0.38284781158482417 1103 6 0.8010095609911474 1103 7 0.3196999252165398 1103 8 0.2051260415407299 1103 9 0.5902289765523676 1103 10 0.8277852671677337 1103 11 1.091116518619927 1103 12 1.1767665924269366 1103 13 0.9745730917932747 1103 14 0.5286270432085161 1103 15 1.4655417467000191 1103 16 0.7639552775658487 1103 17 0.5674649524768327 1103 18 0.378413852856454 1103 19 1.0828306448129084 1103 20 0.9863558436280044 1103 21 1.8614412522370785 1103 22 2.0946743485168042 1103 23 2.030043049369759 1103 24 1.0379245610862071 1103 25 1.3533743458959364 1104 1 0.4638873420506293 1104 2 0.4529678448572376 1104 3 0.6082070836470841 1104 4 -0.03660072956861882 1104 5 0.3390897678267777 1104 6 0.24320806201317424 1104 7 0.043221766385435956 1104 8 0.3063449074654798 1104 9 1.2456911614263149 1104 10 0.5329862664863754 1104 11 0.6647405628322076 1104 12 0.9623673192041338 1104 13 1.242950713112073 1104 14 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2313 17 0.01922580423768494 2313 18 0.3913540657966692 2313 19 0.39061115259341506 2313 20 0.3078955651677262 2313 21 0.1810153718111991 2313 22 0.8573279111703656 2313 23 0.6438022631289755 2313 24 0.6954184185800614 2313 25 0.9497707422923369 2314 1 -0.926666469835574 2314 2 -0.9578856531684785 2314 3 -0.920293473202161 2314 4 -1.655007128272679 2314 5 -0.855402486906435 2314 6 -1.1408660495591647 2314 7 -1.1001416030587237 2314 8 -1.2305083880696017 2314 9 -1.6025421077849842 2314 10 -1.0061450486902928 2314 11 -1.3596595586621731 2314 12 -0.940133440135746 2314 13 -1.233610995908883 2314 14 -0.7841917997308059 2314 15 -1.3514017027735505 2314 16 -0.7783219394101621 2314 17 -0.40534520708030897 2314 18 -0.9747284460689798 2314 19 -1.0300533234927443 2314 20 -0.7625315980305203 2314 21 -0.8037600936871598 2314 22 -0.6486231658650468 2314 23 -1.244514640850582 2314 24 -0.7100945423545824 2314 25 -1.3345666071052555 2315 1 -0.5460077402426435 2315 2 -0.24048350017782028 2315 3 -0.5342522669570648 2315 4 -0.6423886068497069 2315 5 -0.701485041978799 2315 6 -0.7625599256552604 2315 7 -1.310664166686017 2315 8 -0.5961959905505267 2315 9 -1.0369899583588733 2315 10 -0.7925569685591185 2315 11 -1.5530009716514073 2315 12 -1.1662687506084026 2315 13 -1.5059429271842788 2315 14 -1.3433277518232032 2315 15 -0.9837892410925093 2315 16 -1.0461625325519606 2315 17 -1.5660709502898342 2315 18 -1.703786690882552 2315 19 -1.6527096291889052 2315 20 -1.2054987328419529 2315 21 -2.179285865413121 2315 22 -2.337651468424397 2315 23 -2.177750543039217 2315 24 -2.0635200788199732 2315 25 -2.1555547014946477 2316 1 -0.350675354536385 2316 2 -0.4455345728696294 2316 3 -0.5140876442999627 2316 4 -0.9609235313150108 2316 5 -0.970567055796522 2316 6 -0.9655614793229077 2316 7 -0.3096724639323867 2316 8 -0.5776511490576866 2316 9 -0.7393799619951196 2316 10 -0.7486906500903103 2316 11 -1.485862695230793 2316 12 -0.6492941051421397 2316 13 -1.7071924726538512 2316 14 -1.7049025757959626 2316 15 -1.2198475532144215 2316 16 -1.328500306509845 2316 17 -1.4483888901926534 2316 18 -1.3839294960790767 2316 19 -1.691088525754303 2316 20 -1.952845927976 2316 21 -1.984449331083674 2316 22 -2.002059256596688 2316 23 -1.0011613393765302 2316 24 -1.2174984596999465 2316 25 -1.1273242297747075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3356.0 3 0.0 4 52.0 5 502.0 6 629.0 7 493.0 8 405.0 9 387.0 10 361.0 11 308.0 12 317.0 13 287.0 14 276.0 15 303.0 16 318.0 17 304.0 18 369.0 19 409.0 20 486.0 21 639.0 22 817.0 23 1027.0 24 1214.0 25 1412.0 26 1593.0 27 1842.0 28 2189.0 29 2592.0 30 3346.0 31 4188.0 32 5405.0 33 6699.0 34 8400.0 35 11277.0 36 19427.0 37 26801.0 38 30630.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.35947202726511 20.406954914363283 17.06596770932617 14.16760534904543 2 16.97037250107867 24.89572846253416 39.13922048036819 18.994678556018986 3 20.768732921041277 31.610096361282903 31.727311951675535 15.893858766000287 4 11.883359700848555 21.809290953545233 34.836761110312096 31.470588235294116 5 9.930511595787534 40.83127122057893 32.665304713126545 16.572912470506992 6 24.088163382712498 37.97569394505969 20.164677117790884 17.771465554436933 7 20.414209693657416 40.09348482669351 21.82511146267798 17.66719401697109 8 26.345462390335108 31.251258449590104 22.178196461958866 20.22508269811592 9 27.2515460952107 15.920465985905363 22.401121817920323 34.42686610096361 10 20.859341291528835 29.757658564648352 26.3080684596577 23.074931684165108 11 28.883934992089742 27.78440960736373 21.13260463109449 22.199050769452036 12 25.046023299295268 31.876168560333667 27.444987775061126 15.632820365309938 13 31.41953113763843 24.43837192578743 28.490579605925497 15.65151733064864 14 23.18423701999137 29.122680857183948 30.711922910973684 16.981159211851 15 26.3015964331943 28.125988781820798 30.976556881921475 14.595857903063425 16 16.75535739968359 32.393211563353944 30.53214439810154 20.319286638860923 17 16.592118509995686 31.59499496620164 30.391917158061272 21.420969365741406 18 15.253847260175462 25.397670070473175 39.64835322882209 19.700129440529267 19 16.917877175320008 29.054365022292533 39.01769020566662 15.01006759672084 20 18.45030921904214 24.235581763267653 41.08873867395369 16.225370343736518 21 19.83963756651805 26.838774629656264 38.9493743707752 14.372213433050481 22 18.599165827700272 26.64029915144542 38.92492449302459 15.835610527829713 23 19.280166834459944 26.286495038113046 39.320437221343305 15.112900906083704 24 17.830432906658995 26.886236157054512 38.672515460952106 16.610815475334388 25 17.595915285318757 28.521807917730396 39.72313113516235 14.1591456617885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9662.0 1 9662.0 2 5736.0 3 1810.0 4 1810.0 5 1810.0 6 2247.0 7 2684.0 8 2684.0 9 2684.0 10 2247.0 11 1810.0 12 1810.0 13 1810.0 14 1222.5 15 635.0 16 635.0 17 635.0 18 1400.5 19 2166.0 20 2166.0 21 2166.0 22 3940.0 23 5714.0 24 5714.0 25 5714.0 26 8124.5 27 10535.0 28 10535.0 29 10535.0 30 11018.0 31 11501.0 32 11501.0 33 11501.0 34 10959.5 35 10418.0 36 10418.0 37 10418.0 38 8787.0 39 7156.0 40 7156.0 41 7156.0 42 9817.0 43 12478.0 44 12478.0 45 12478.0 46 14546.0 47 16614.0 48 16614.0 49 16614.0 50 16004.5 51 15395.0 52 15395.0 53 15395.0 54 13389.5 55 11384.0 56 11384.0 57 11384.0 58 9396.0 59 7408.0 60 7408.0 61 7408.0 62 6982.0 63 6556.0 64 6556.0 65 6556.0 66 4607.0 67 2658.0 68 2658.0 69 2658.0 70 2173.0 71 1688.0 72 1688.0 73 1688.0 74 1136.5 75 585.0 76 585.0 77 585.0 78 374.0 79 163.0 80 163.0 81 163.0 82 97.5 83 32.0 84 32.0 85 32.0 86 20.0 87 8.0 88 8.0 89 8.0 90 4.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40845678124550555 2 0.0 3 0.0 4 0.0 5 0.03164101826549691 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0035955702574428307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 139060.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.64662735509852 #Duplication Level Percentage of deduplicated Percentage of total 1 81.32844663118787 23.297857040126562 2 9.865448338186566 5.652236444700129 3 3.070087358168491 2.638429454911549 4 1.2651872677979716 1.4497339278009491 5 0.8384375941359575 1.2009204659859054 6 0.44181142685008534 0.7593844383719257 7 0.31880710914750476 0.6392923917733353 8 0.2686012651872678 0.6155616280742126 9 0.15814840847474645 0.407737667194017 >10 1.722060447836128 10.293398533007334 >50 0.3589717843156944 7.043722134330505 >100 0.31378652475148106 17.140083417229974 >500 0.03012350637614218 6.197324895728463 >1k 0.01506175318807109 14.371494318998993 >5k 0.005020584396023697 8.292823241766143 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6432 4.625341579174457 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 5100 3.6674816625916873 No Hit GGTATCAACGCAGAGTACTTTTTTT 4693 3.3748022436358402 No Hit TATCAACGCAGAGTACTTTTTTTTT 4620 3.3223069178771754 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 4423 3.180641449733928 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2628 1.8898317273119518 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2116 1.5216453329498059 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1505 1.082266647490292 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 822 0.5911117503236013 No Hit GTACATAAGCAGTGGTATCAACGCA 816 0.58679706601467 No Hit GTGGTATCAACGCAGAGTACTTTTT 800 0.5752912411908528 No Hit GGTATCAACGCAGAAAAAAAAAAAA 788 0.56666187257299 No Hit GTACTGGTTCACTATCGGTCAGTCA 774 0.5565942758521502 No Hit GGTATCAACGCAGAGAAAAAAAAAA 756 0.543650222925356 No Hit ATACAGGGTGACAGCCCCGTACACA 719 0.5170430030202791 No Hit ATCAACGCAGAGTACTTTTTTTTTT 688 0.4947504674241335 No Hit GTGGTATCAACGCAGAGTACATGGG 685 0.49259312526966775 No Hit GGATACCACGTGTCCCGCCCTACTC 655 0.4710197037250108 No Hit GTATCAACGCAGAGAAAAAAAAAAA 571 0.4106141233999713 No Hit ATCATTAACTGAATCCATAGGTTAA 544 0.3911980440097799 No Hit GGTATCAACGCAGAGTACATGGGGG 435 0.3128146123975262 No Hit AGCGTACACGGTGGATGCCCTGGCA 429 0.30849992808859483 No Hit GGTTAATGAGGCGAACCGGGGGAAC 418 0.3005896735222206 No Hit TATCAACGCAGAGAAAAAAAAAAAA 387 0.2782971379260751 No Hit GCTTATGTACTCTGCGTTGATACCA 384 0.27613979577160935 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 380 0.27326333956565513 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 375 0.2696677693082123 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 370 0.26607219905076945 No Hit GTTAATGATAGTGTGTCGAAACACA 365 0.2624766287933266 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 362 0.2603192866388609 No Hit GTGGATGCCCTGGCAGTCAGAGGCG 347 0.2495325758665324 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 339 0.24377966345462393 No Hit GTACATGGGGGTTAAGCGACTAAGC 324 0.23299295268229542 No Hit GTATCAACGCAGAAAAAAAAAAAAA 324 0.23299295268229542 No Hit GTATTTAGCCTTGGAGGATGGTCCC 315 0.2265209262188983 No Hit GTATCAACGCAGAGTACATGGGGGT 314 0.22580181216740974 No Hit CCCATATTCAGACAGGATACCACGT 311 0.22364447001294407 No Hit CAACGCAGAGTACTTTTTTTTTTTT 295 0.21213864518912698 No Hit TATTCAGACAGGATACCACGTGTCC 286 0.20566661872572992 No Hit GTTATAACCGGCGATTTCCGAATGG 282 0.20279016251977564 No Hit GGTATCAACGCAGAGTACATGGGGA 278 0.19991370631382135 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 277 0.19919459226233283 No Hit AACGCAGAGTACTTTTTTTTTTTTT 276 0.19847547821084421 No Hit GCCTTGGAGGATGGTCCCCCCATAT 275 0.19775636415935569 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 269 0.19344167985042426 No Hit CTTATGTACTCTGCGTTGATACCAC 267 0.19200345174744715 No Hit GAGTACATAAGCAGTGGTATCAACG 266 0.19128433769595857 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 263 0.18912699554149287 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 260 0.1869696533870272 No Hit TATGTACTCTGCGTTGATACCACTG 259 0.18625053933553862 No Hit GTTCACTATCGGTCAGTCAGGAGTA 255 0.18337408312958436 No Hit CATCTAAGTACCCCGAGGAAAAGAA 247 0.17762117071767583 No Hit GTACTTAGATGTTTCAGTTCCCCCG 237 0.17043003020279016 No Hit CACACACACTGATTCAGGCTCTGGG 237 0.17043003020279016 No Hit TCATTAACCTATGGATTCAGTTAAT 236 0.1697109161513016 No Hit ACGCAGAGTACATGGGGGTTAAGCG 232 0.16683445994534735 No Hit TCAACGCAGAGTACTTTTTTTTTTT 229 0.16467711779088165 No Hit CGGTACTGGTTCACTATCGGTCAGT 225 0.16180066158492737 No Hit GTGCTAATCTGCGATAAGCGTCGGT 218 0.15676686322450742 No Hit CACTATCATTAACTGAATCCATAGG 218 0.15676686322450742 No Hit TCACGGTACTGGTTCACTATCGGTC 213 0.15317129296706458 No Hit CTATCGGTCAGTCAGGAGTATTTAG 208 0.14957572270962174 No Hit TCGGTAAGGTGATATGAACCGTTAT 204 0.1466992665036675 No Hit GATATGAACCGTTATAACCGGCGAT 201 0.1445419243492018 No Hit GAATCAGTGTGTGTGTTAGTGGAAG 198 0.1423845821947361 No Hit AGCTCACAGCATGTGCATTTTTGTG 196 0.14094635409175896 No Hit GTGGTATCCTGTCTGAATATGGGGG 193 0.13878901193729323 No Hit ATCCATAGGTTAATGAGGCGAACCG 189 0.135912555731339 No Hit CATAAGCAGTGGTATCAACGCAGAG 184 0.13231698547389617 No Hit GGTGATATGAACCGTTATAACCGGC 184 0.13231698547389617 No Hit GGGTACTTAGATGTTTCAGTTCCCC 180 0.1294405292679419 No Hit TATCAACGCAGAGTACATGGGGGTT 174 0.1251258449590105 No Hit CTATCATTAACTGAATCCATAGGTT 171 0.1229685028045448 No Hit GTACATGGGGAAAAAAAAAAAAAAA 170 0.12224938875305623 No Hit GGAGTATTTAGCCTTGGAGGATGGT 169 0.12153027470156767 No Hit GCCTGAATCAGTGTGTGTGTTAGTG 168 0.1208111606500791 No Hit GTATCAACGCAGAGTACATGGGGAA 168 0.1208111606500791 No Hit CCTATGGATTCAGTTAATGATAGTG 167 0.12009204659859053 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 164 0.11793470444412482 No Hit GGTTCACTATCGGTCAGTCAGGAGT 164 0.11793470444412482 No Hit ACGCAGAGTACATAAGCAGTGGTAT 162 0.11649647634114771 No Hit TATCAACGCAGAAAAAAAAAAAAAA 156 0.11218179203221632 No Hit ACGCAGAGAAAAAAAAAAAAAAAAA 155 0.11146267798072775 No Hit GGTATCAACGCAGAGTACATGGGAA 154 0.11074356392923917 No Hit GTGTTAGTGGAAGCGTCTGGAAAGG 153 0.1100244498777506 No Hit TTCCAGACGCTTCCACTAACACACA 152 0.10930533582626203 No Hit GGGTGACAGCCCCGTACACAAAAAT 152 0.10930533582626203 No Hit GAGTACATGGGGGTTAAGCGACTAA 151 0.10858622177477348 No Hit GCGTACACGGTGGATGCCCTGGCAG 149 0.10714799367179635 No Hit GTACATGGGGGGTCTTGAGGGGTTT 148 0.1064288796203078 No Hit CAGTAGCGGCGAGCGAACGGGGAGC 146 0.10499065151733065 No Hit GGGTTTCCCCATTCGGAAATCGCCG 141 0.10139508125988782 No Hit ATGATAGTGTGTCGAAACACACTGG 141 0.10139508125988782 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTGAT 35 9.880025E-5 18.994246 17 GTTGATT 40 2.7448367E-4 16.619967 18 GCGCGCG 40 2.7448367E-4 16.619967 11 CGCGCGA 40 2.7448367E-4 16.619967 12 GTGTGTC 70 4.3671935E-7 14.92405 11 GGCGCGC 45 6.723139E-4 14.773303 10 TCGGTTG 50 0.0014910062 13.295973 15 TTGATTT 50 0.0014910062 13.295973 19 CGGTTGA 50 0.0014910062 13.295973 16 GTGTCGA 80 1.9690433E-6 13.058545 13 TAGTGTG 80 1.9690433E-6 13.058545 9 TGTCGAA 80 1.9690433E-6 13.058545 14 CCTACTC 130 1.9099389E-10 12.419315 19 CCCTACT 130 1.9099389E-10 12.419315 18 CGCCCTA 115 5.169568E-9 12.387553 16 CCGCCCT 110 3.718924E-8 12.087248 15 AGGCGCG 55 0.0030512728 12.087248 9 TCTCGGT 55 0.0030512728 12.087248 13 ATGATAG 90 7.3201263E-6 11.611771 5 TAATGAT 90 7.3201263E-6 11.611771 3 >>END_MODULE