FastQCFastQC Report
Thu 26 May 2016
SRR1512170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327572
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61230.26306382788588284No Hit
GGTATCAACGCAGAGTACTTTTTTT37960.16308840285069592No Hit
TATCAACGCAGAGTACTTTTTTTTT37630.16167061641916985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG655.8646183E-513.0526317
TCCAACG2200.012.85624518
TGTAGGA10800.011.7697372
GTATTAG1950.011.4727791
TCGCGCG752.2297993E-411.3117949
GTAGGAC10650.011.2414673
CGGTTTC1701.4551915E-1111.09070913
GTATCGC855.761008E-511.090476
ATCGCGC855.7635283E-511.0899948
TAGGACC4300.010.9615134
CGTGCGC951.4831885E-510.91488810
GCGTGCG1053.8061062E-610.7731379
CGCGCCT804.0503673E-410.60549112
TAGAACA8100.010.4743344
GTGCTAC2051.0913936E-1110.4170791
GTAGAAC7300.010.330853
GGTATCA16300.010.2314411
GAACAGT5450.010.2052696
GTCCTAC10450.010.12039851
GTCCTAA5600.09.9874641
Produced by FastQC (version 0.11.5)