FastQCFastQC Report
Thu 26 May 2016
SRR1512170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327572
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84690.3638555541998271No Hit
TATCAACGCAGAGTACTTTTTTTTT56290.2418399946381895No Hit
GGTATCAACGCAGAGTACTTTTTTT54090.23238808509468234No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28350.12180074343564881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4750.011.8005744
AATCCCG2900.011.7908919
TAGGGGT1604.5474735E-1111.2817984
ATAGGAC2600.010.9620713
GGACGTG10500.010.8541736
GGTATCA19150.010.6540221
TGTAGGA11350.010.6304732
AGGACGT10650.010.615545
TAGGACG10550.010.5360574
GAGTAAG2100.010.4418431
CTGTAGG10750.010.3763821
TGCACCG1106.044722E-610.3641415
ACTGTTC8300.010.2983878
GATATAC6950.010.2883341
GTAGGAC10900.010.2849043
CGCTCAC750.002654436710.1305610
GACGTGA5650.010.0857357
TCCAACG2107.2759576E-129.94965718
TTAGGAC7000.09.9076253
TAGAACA7250.09.8280644
Produced by FastQC (version 0.11.5)