##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512170_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2327572 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.91396055632221 31.0 30.0 34.0 26.0 34.0 2 30.117728688951406 31.0 30.0 34.0 26.0 34.0 3 30.185890704992154 31.0 30.0 34.0 26.0 34.0 4 33.81219571295754 35.0 35.0 37.0 30.0 37.0 5 33.30462258525193 35.0 35.0 37.0 27.0 37.0 6 33.20205948516308 35.0 33.0 37.0 27.0 37.0 7 32.97053238310136 35.0 33.0 37.0 26.0 37.0 8 32.84346177046295 35.0 33.0 37.0 26.0 37.0 9 34.18886891576286 38.0 34.0 39.0 27.0 39.0 10 33.951389688482244 37.0 34.0 39.0 25.0 39.0 11 33.94546463009522 37.0 34.0 39.0 25.0 39.0 12 33.698241772972004 37.0 33.0 39.0 25.0 39.0 13 33.65658205202675 37.0 33.0 39.0 25.0 39.0 14 34.48143172370178 38.0 33.0 40.0 24.0 41.0 15 34.36638265110596 38.0 33.0 40.0 23.0 41.0 16 34.23809360140095 38.0 33.0 40.0 22.0 41.0 17 34.13749392070363 38.0 33.0 40.0 19.0 41.0 18 34.048429436339674 38.0 33.0 40.0 19.0 41.0 19 34.059513089176185 38.0 33.0 40.0 19.0 41.0 20 33.89825577898342 38.0 33.0 40.0 18.0 41.0 21 33.766388322251686 38.0 33.0 40.0 18.0 41.0 22 33.84622301694642 38.0 33.0 40.0 18.0 41.0 23 33.70276880801109 38.0 33.0 40.0 16.0 41.0 24 33.51435659133208 38.0 33.0 40.0 10.0 41.0 25 32.97480335731827 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3842911765749619 1101 2 0.31560399036041176 1101 3 0.40522993597200596 1101 4 0.45179028331801874 1101 5 0.5764840993802451 1101 6 0.49068472929700135 1101 7 0.3680360466110528 1101 8 0.22028090780595022 1101 9 0.42114827146305345 1101 10 0.3937462163854306 1101 11 0.5128675572799466 1101 12 0.47006342471635776 1101 13 0.3420519182358248 1101 14 0.14505701124808468 1101 15 0.28807381004602917 1101 16 0.2829241735003194 1101 17 0.3671566449033534 1101 18 0.3097050651033868 1101 19 0.26677250258221363 1101 20 0.2853100882335795 1101 21 0.09705633044048767 1101 22 0.13168882199345688 1101 23 0.047876448806533745 1101 24 0.027821310917730102 1101 25 0.2486417997035062 1102 1 0.5211194274187463 1102 2 0.6314068348188115 1102 3 0.573678999720574 1102 4 0.6538149832791547 1102 5 0.6394136732858158 1102 6 0.6547574086742003 1102 7 0.775436079922116 1102 8 0.7060607216851764 1102 9 0.9689475397683438 1102 10 0.8889329593484732 1102 11 1.0404022132283615 1102 12 1.151549557550922 1102 13 1.0312417127984617 1102 14 1.111238242570444 1102 15 1.2789942482970815 1102 16 1.0447050900905737 1102 17 1.2547396593247413 1102 18 1.3825693761291546 1102 19 1.2485427366594308 1102 20 1.1571375445893821 1102 21 1.1321037795597562 1102 22 1.3803137834655814 1102 23 1.1305351693171488 1102 24 1.351949432154683 1102 25 1.2146166555948739 1103 1 0.4409078150157306 1103 2 0.35604065068210033 1103 3 0.37186331535368566 1103 4 0.4981917107606222 1103 5 0.36079390651794796 1103 6 0.4150475603291355 1103 7 0.39405476570360065 1103 8 0.4956172749787413 1103 9 0.5478396032781632 1103 10 0.5444842903595486 1103 11 0.4483429900848179 1103 12 0.6831995341606074 1103 13 0.6009871405056373 1103 14 0.5660942011351153 1103 15 0.848081578950314 1103 16 0.9115415461699072 1103 17 0.4279541214315117 1103 18 0.8279995062016354 1103 19 0.8165300704810718 1103 20 0.5488877547294067 1103 21 0.621883798831135 1103 22 0.83225182430823 1103 23 1.0665554993161948 1103 24 0.538833339808626 1103 25 0.6877327106655429 1104 1 0.47295113961234136 1104 2 0.385964059294416 1104 3 0.45364955471817936 1104 4 0.5309904825217586 1104 5 0.6162364613502334 1104 6 0.6390265271182045 1104 7 0.5882198718539584 1104 8 0.7331926637791426 1104 9 0.8064758611627667 1104 10 0.7737269830487392 1104 11 0.7013163769773527 1104 12 0.9849233962581536 1104 13 0.9054464480431292 1104 14 1.2778821596435463 1104 15 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2313 25 -0.4478502932495232 2314 1 -0.5041195479676723 2314 2 -0.6668605495382671 2314 3 -0.6108385698203804 2314 4 -0.5289075371968934 2314 5 -0.8992742372051197 2314 6 -0.7443424213093621 2314 7 -0.7665230596959489 2314 8 -0.7604238181064318 2314 9 -0.9722387657956375 2314 10 -1.0971602604524477 2314 11 -0.8520309971193569 2314 12 -0.8563756069981849 2314 13 -0.9385214634754249 2314 14 -0.9579214106242446 2314 15 -0.9750512258089188 2314 16 -0.8967891498089671 2314 17 -0.9511347260411469 2314 18 -0.9702016801596898 2314 19 -0.9050773436221888 2314 20 -0.8349757385620578 2314 21 -0.9032381227901425 2314 22 -0.6822818948717853 2314 23 -0.7455066222751512 2314 24 -0.7835046387160673 2314 25 -0.9014177991885148 2315 1 -0.7605943198859713 2315 2 -0.7585168818638621 2315 3 -0.7040122068604475 2315 4 -0.8323906011670203 2315 5 -1.0563409154909351 2315 6 -1.054095804828492 2315 7 -1.226890470658617 2315 8 -0.9988424293050571 2315 9 -1.0952011130429042 2315 10 -1.1607436066338792 2315 11 -1.1894370663382148 2315 12 -1.2905258560278767 2315 13 -1.3647919170915515 2315 14 -1.5719739917516193 2315 15 -1.4011467218237783 2315 16 -1.6128480454980405 2315 17 -1.685360722997821 2315 18 -1.6616713240103635 2315 19 -1.403502054875112 2315 20 -1.4108379577752146 2315 21 -1.502511814127395 2315 22 -1.2956832449277584 2315 23 -1.4302142553870496 2315 24 -1.3311142218343335 2315 25 -1.2996923552587347 2316 1 -1.1209727818823083 2316 2 -1.3585199964925785 2316 3 -1.2103961575247304 2316 4 -1.199888066205311 2316 5 -1.440441081950535 2316 6 -1.6008733753488755 2316 7 -1.7853004873045784 2316 8 -1.6676819532513605 2316 9 -2.093453806331567 2316 10 -2.1305618161376536 2316 11 -2.0110245579892805 2316 12 -2.1405111480589234 2316 13 -2.1828937931362518 2316 14 -2.4303327900586424 2316 15 -2.510215828513651 2316 16 -2.460104230631572 2316 17 -2.412098426270603 2316 18 -2.3703739427209776 2316 19 -2.2843951919637675 2316 20 -2.2320779952891883 2316 21 -2.3148024851222395 2316 22 -2.4798353218660125 2316 23 -2.4284579508594177 2316 24 -2.223932580148144 2316 25 -2.4890264822456096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62792.0 3 0.0 4 1104.0 5 9586.0 6 11779.0 7 9266.0 8 7645.0 9 6735.0 10 6315.0 11 6135.0 12 5740.0 13 5509.0 14 5246.0 15 5356.0 16 5397.0 17 5617.0 18 5998.0 19 7005.0 20 8451.0 21 10400.0 22 13453.0 23 16896.0 24 20238.0 25 24096.0 26 27722.0 27 31385.0 28 36068.0 29 44037.0 30 55118.0 31 70344.0 32 88848.0 33 112149.0 34 143467.0 35 187269.0 36 265004.0 37 425900.0 38 579502.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.95719809788492 19.817206499209465 14.457998121346524 23.76759728155909 2 16.184934343599252 20.904487594798358 38.813750981709696 24.096827079892694 3 19.650262161600157 24.35911756972502 29.901803252488 26.088817016186827 4 14.002660282904245 17.31508198242632 36.565313554210135 32.11694418045929 5 14.078688828950558 37.34801249421962 34.939204997687845 13.634093679141976 6 33.08769825380268 36.70739293993913 16.969786541511926 13.235122264746268 7 28.97835169008735 31.606584028335106 20.9995222489358 18.415542032641742 8 26.06385538234693 36.617384983149826 19.429774889885255 17.888984744617996 9 26.316994705212128 15.806127587030605 19.620187903961725 38.25668980379554 10 15.520722882041888 29.34641764035656 32.95906635756059 22.17379312004097 11 35.37158034209039 22.727331313488904 22.95336084125432 18.94772750316639 12 23.464494331432068 24.695906292050257 30.51037733741427 21.32922203910341 13 29.0752767261335 21.370123029491676 25.74261934754328 23.811980896831546 14 21.74729718350281 21.42696337642831 27.013084879866227 29.81265456020265 15 23.77318510447797 29.623874148683694 23.30428446466962 23.298656282168714 16 23.19021710176957 28.212875906738866 26.097968183153945 22.498938808337616 17 22.4378880653316 28.110838246894186 26.970852029496832 22.48042165827738 18 22.277592272118756 27.533025831209518 28.351690087352832 21.837691809318898 19 23.653618448752606 26.924322856607656 27.412428058079406 22.009630636560328 20 23.707923965402575 26.925654716588788 27.718970669865424 21.647450648143217 21 23.719008477503596 26.403522640760414 27.25419449967606 22.623274382059932 22 23.49031522977592 26.74722844234249 27.509696799927134 22.252759527954453 23 23.41229401281679 26.58521412012174 27.37547968440933 22.627012182652138 24 23.336077251315963 26.4652178321444 27.648167274739517 22.550537641800126 25 23.429757303766657 26.5955714436097 27.675362368656803 22.29930888396684 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 314.0 1 314.0 2 501.0 3 688.0 4 688.0 5 688.0 6 1721.5 7 2755.0 8 2755.0 9 2755.0 10 3982.5 11 5210.0 12 5210.0 13 5210.0 14 7804.0 15 10398.0 16 10398.0 17 10398.0 18 17824.5 19 25251.0 20 25251.0 21 25251.0 22 39466.5 23 53682.0 24 53682.0 25 53682.0 26 76132.0 27 98582.0 28 98582.0 29 98582.0 30 125629.0 31 152676.0 32 152676.0 33 152676.0 34 175129.0 35 197582.0 36 197582.0 37 197582.0 38 218890.5 39 240199.0 40 240199.0 41 240199.0 42 260571.0 43 280943.0 44 280943.0 45 280943.0 46 291022.0 47 301101.0 48 301101.0 49 301101.0 50 298800.0 51 296499.0 52 296499.0 53 296499.0 54 272332.5 55 248166.0 56 248166.0 57 248166.0 58 214259.0 59 180352.0 60 180352.0 61 180352.0 62 149018.0 63 117684.0 64 117684.0 65 117684.0 66 91831.0 67 65978.0 68 65978.0 69 65978.0 70 48420.0 71 30862.0 72 30862.0 73 30862.0 74 21574.0 75 12286.0 76 12286.0 77 12286.0 78 8391.0 79 4496.0 80 4496.0 81 4496.0 82 2950.5 83 1405.0 84 1405.0 85 1405.0 86 888.0 87 371.0 88 371.0 89 371.0 90 223.0 91 75.0 92 75.0 93 75.0 94 45.5 95 16.0 96 16.0 97 16.0 98 8.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.381943072008084 2 0.0 3 0.0 4 0.0 5 0.03162093374555116 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007604490860003472 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2327572.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.50703128892575 #Duplication Level Percentage of deduplicated Percentage of total 1 84.55131045257399 61.30564512504456 2 10.149538364047238 14.718257914602509 3 2.601830920138936 5.659531078050249 4 1.0102974301563172 2.9301466951786153 5 0.5114886481176196 1.854326170649729 6 0.29212344677534147 1.270860233734111 7 0.19385371717718147 0.9839030275788321 8 0.13107516594260704 0.7603096926561395 9 0.09935300426218276 0.6483412249818173 >10 0.4285360688000336 5.253870846493402 >50 0.018125015972664346 0.9096005414489143 >100 0.010392999888535552 1.4393104803007568 >500 0.0011263016228820332 0.5676562326599476 >1k 7.706274261787078E-4 0.8598012628219123 >5k 1.778370983489326E-4 0.8384394737985924 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8469 0.3638555541998271 No Hit TATCAACGCAGAGTACTTTTTTTTT 5629 0.2418399946381895 No Hit GGTATCAACGCAGAGTACTTTTTTT 5409 0.23238808509468234 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2835 0.12180074343564881 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.592645039551945E-5 0.0 0.0 0.0 0.0 7 8.592645039551945E-5 0.0 0.0 4.296322519775973E-5 0.0 8 8.592645039551945E-5 0.0 0.0 4.296322519775973E-5 0.0 9 8.592645039551945E-5 0.0 0.0 4.296322519775973E-5 0.0 10 8.592645039551945E-5 0.0 0.0 4.296322519775973E-5 0.0 11 8.592645039551945E-5 0.0 0.0 4.296322519775973E-5 0.0 12 8.592645039551945E-5 0.0 0.0 4.296322519775973E-5 0.0 13 8.592645039551945E-5 0.0 0.0 4.296322519775973E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 475 0.0 11.800574 4 AATCCCG 290 0.0 11.79089 19 TAGGGGT 160 4.5474735E-11 11.281798 4 ATAGGAC 260 0.0 10.962071 3 GGACGTG 1050 0.0 10.854173 6 GGTATCA 1915 0.0 10.654022 1 TGTAGGA 1135 0.0 10.630473 2 AGGACGT 1065 0.0 10.61554 5 TAGGACG 1055 0.0 10.536057 4 GAGTAAG 210 0.0 10.441843 1 CTGTAGG 1075 0.0 10.376382 1 TGCACCG 110 6.044722E-6 10.364141 5 ACTGTTC 830 0.0 10.298387 8 GATATAC 695 0.0 10.288334 1 GTAGGAC 1090 0.0 10.284904 3 CGCTCAC 75 0.0026544367 10.13056 10 GACGTGA 565 0.0 10.085735 7 TCCAACG 210 7.2759576E-12 9.949657 18 TTAGGAC 700 0.0 9.907625 3 TAGAACA 725 0.0 9.828064 4 >>END_MODULE