FastQCFastQC Report
Thu 26 May 2016
SRR1512169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773044
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT52640.2968905452994962No Hit
CTGTAGGACGTGGAATATGGCAAGA45790.25825642228844853No Hit
GTCCTAAAGTGTGTATTTCTCATTT42440.23936236212976103No Hit
CTTTAGGACGTGAAATATGGCGAGG36940.20834226336176653No Hit
GTATCAACGCAGAGTACTTTTTTTT26690.15053207929414048No Hit
GTCCTACAGTGTGCATTTCTCATTT20970.11827117657542623No Hit
CTGAAGGACCTGGAATATGGCGAGA18870.10642713886401016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG400.005568621714.1359035
CGGTAGG809.032883E-714.0182661
ACCGATG500.001593658213.1931388
TAGGACC7850.012.8453654
AGGACGG1001.6020385E-712.2511175
GGCGAGG10900.011.84676819
GCCGGTT1053.0216688E-711.66707211
GGTATCA8800.011.5853441
GATATAC3300.011.4308731
AGGACGT26600.011.1953535
TGTCGAG1451.3860699E-911.05062218
TTAGGAC15850.010.9995473
TAGAACG600.006238419610.9945934
GGACGTG26900.010.8953316
ATAGGAC2950.010.86148553
GTCCTAC25650.010.8111811
GTCCTAG2650.010.7721841
TGGACCG700.00159500110.7702125
GTCTTAG1801.09139364E-1010.76149751
GTCTAAT1051.8847924E-510.6805851
Produced by FastQC (version 0.11.5)