FastQCFastQC Report
Thu 26 May 2016
SRR1512169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773044
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT50900.28707691405289437No Hit
CTGTAGGACGTGGAATATGGCAAGA50520.28493370722892386No Hit
GTCCTAAAGTGTGTATTTCTCATTT42890.24190037021077876No Hit
CTTTAGGACGTGAAATATGGCGAGG42640.24049036572132446No Hit
GTATCAACGCAGAGTACTTTTTTTT35040.19762622924191392No Hit
GGTATCAACGCAGAGTACTTTTTTT22920.12926921159316973No Hit
TATCAACGCAGAGTACTTTTTTTTT22850.1288744103361225No Hit
CTGAAGGACCTGGAATATGGCGAGA22470.126731203512152No Hit
GTCCTACAGTGTGCATTTCTCATTT21830.123121592019149No Hit
CTGTAGGACCTGGAATATGGCGAGA18280.10309952826889801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1400.013.56750118
TAGGACC8400.012.3276374
CCAACGA1052.7277383E-711.75949619
TGTAGGA28500.011.4335712
CTGTAGG28450.011.1964031
GGCGAGG12250.011.16505919
TGGACCG600.005875311.0835645
GGACCGT600.00588888411.0801266
GACGTGG15050.011.0433147
GTAGGAC28850.010.8997073
AGGACGT30900.010.8837215
AATCCCG1752.0008883E-1110.854918519
GGACGTG31200.010.7757276
ATAGGAC2750.010.7093133
GTACTAG1002.2979959E-510.4907611
CACCTTT6250.010.48496314
GCCGGTT1002.4065708E-510.44697611
CGGTTTC1106.0665243E-610.36063713
ACTGTTC4650.010.2120978
ACCTTTT6800.010.19557715
Produced by FastQC (version 0.11.5)