##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512169_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1773044 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.28938819341201 31.0 30.0 34.0 26.0 34.0 2 30.50156679698868 31.0 30.0 34.0 27.0 34.0 3 30.5840864637313 31.0 30.0 34.0 27.0 34.0 4 34.20083539945991 37.0 35.0 37.0 30.0 37.0 5 33.72751437640577 37.0 35.0 37.0 28.0 37.0 6 33.63245920575011 37.0 35.0 37.0 28.0 37.0 7 33.42526355803917 37.0 35.0 37.0 28.0 37.0 8 33.307277202370614 36.0 35.0 37.0 27.0 37.0 9 34.727921585702326 38.0 35.0 39.0 27.0 39.0 10 34.49444683831873 38.0 35.0 39.0 27.0 39.0 11 34.47056474627815 38.0 35.0 39.0 27.0 39.0 12 34.26526527260463 38.0 34.0 39.0 27.0 39.0 13 34.24500463609476 38.0 34.0 39.0 27.0 39.0 14 35.14054811950521 39.0 34.0 40.0 26.0 41.0 15 35.064303536742464 39.0 34.0 40.0 26.0 41.0 16 34.94096818804271 38.0 34.0 40.0 25.0 41.0 17 34.85749930627779 38.0 34.0 40.0 25.0 41.0 18 34.77031985669842 38.0 34.0 40.0 25.0 41.0 19 34.78345545852218 39.0 34.0 40.0 25.0 41.0 20 34.65141192209556 39.0 34.0 40.0 24.0 41.0 21 34.51487723936913 38.0 34.0 40.0 24.0 41.0 22 34.60312321634432 39.0 34.0 40.0 24.0 41.0 23 34.476623817570236 39.0 34.0 40.0 23.0 41.0 24 34.32160397598706 39.0 34.0 40.0 20.0 41.0 25 33.832048161241346 38.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2816121385446806 1101 2 0.22348192089361163 1101 3 0.28405858242755144 1101 4 0.3731622044612166 1101 5 0.33898779452152894 1101 6 0.39759123483622716 1101 7 0.2873756042448363 1101 8 0.12183238822117204 1101 9 0.25255553201086656 1101 10 0.2871916330435553 1101 11 0.2327563837801847 1101 12 0.3885633934773338 1101 13 0.38998947846125986 1101 14 0.2081500531051219 1101 15 -0.012529254223302644 1101 16 0.1827709797508561 1101 17 0.306206850342015 1101 18 0.28199217307506075 1101 19 0.21981950424034125 1101 20 0.23073911388102175 1101 21 0.22046544818064007 1101 22 0.3043912595682343 1101 23 0.12740635298366243 1101 24 0.15715131370064483 1101 25 0.03241065893216444 1102 1 0.6332731199427712 1102 2 0.6299687132308414 1102 3 0.5850246275889432 1102 4 0.6699141231267376 1102 5 0.7952768187996995 1102 6 0.8901308289927456 1102 7 0.6812012764309898 1102 8 0.7767472173117298 1102 9 1.0070326584905231 1102 10 0.9498953701841302 1102 11 1.0584195599982849 1102 12 0.9573407273656755 1102 13 0.9733650683867126 1102 14 1.161738439660141 1102 15 1.396118480769303 1102 16 1.3753828335808862 1102 17 1.309296537030562 1102 18 1.3064018872258814 1102 19 1.5379786665326876 1102 20 1.3990918996626291 1102 21 1.2846439313762161 1102 22 1.2321034750570377 1102 23 1.270817117692836 1102 24 1.4709262419365174 1102 25 1.3417617094004157 1103 1 0.4524389672059641 1103 2 0.5023927135586028 1103 3 0.47448709890982244 1103 4 0.4929987675056253 1103 5 0.5968930921526976 1103 6 0.5263505110641944 1103 7 0.6769793000298066 1103 8 0.5368760685080574 1103 9 0.837398726992653 1103 10 0.5681895770015686 1103 11 0.5893417986085794 1103 12 0.7061525923279817 1103 13 0.6804764662890435 1103 14 0.6484661518856356 1103 15 0.7143512323261731 1103 16 0.8034248689791212 1103 17 0.44830423942321573 1103 18 0.9102356712311277 1103 19 0.8188722260213765 1103 20 0.6936080419110695 1103 21 0.8666600736246579 1103 22 0.8723587192392728 1103 23 1.0111307443726076 1103 24 0.8519969974265251 1103 25 0.8642358981909766 1104 1 0.22793335299446582 1104 2 0.22778602259161929 1104 3 0.27252212488602723 1104 4 0.24133859488957654 1104 5 0.43294449989527806 1104 6 0.42283848659629086 1104 7 0.5648469470886326 1104 8 0.3740613165388922 1104 9 0.6304506165518973 1104 10 0.47043054817635976 1104 11 0.5707028259254869 1104 12 0.6541582903336831 1104 13 0.8397928103480297 1104 14 0.960720006418903 1104 15 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-0.34137206313836543 2315 3 -0.4798921109409875 2315 4 -0.454977005948912 2315 5 -0.6183032630091319 2315 6 -0.5905042116887813 2315 7 -0.8372696577607073 2315 8 -0.6591711952095451 2315 9 -0.7899188019093586 2315 10 -0.6512482933205561 2315 11 -0.6679036079496257 2315 12 -0.7344004542047387 2315 13 -0.8624076192686374 2315 14 -0.8940249941587908 2315 15 -0.947826635679931 2315 16 -1.1805240193307682 2315 17 -1.0034296401050824 2315 18 -1.0073323739505895 2315 19 -1.1449996545877354 2315 20 -1.0099129132546238 2315 21 -1.0645727974694736 2315 22 -0.9911365666928305 2315 23 -1.1037043959396655 2315 24 -0.9656202632030499 2315 25 -0.9133880813122417 2316 1 -1.1802858458879761 2316 2 -1.080912237300879 2316 3 -1.1028422955336659 2316 4 -1.1482951625886386 2316 5 -1.3590435653359734 2316 6 -1.323338895042994 2316 7 -1.3579490685814477 2316 8 -1.4318126851010007 2316 9 -1.658223500487793 2316 10 -1.6622070964529954 2316 11 -1.5902571181967176 2316 12 -1.7790502796198524 2316 13 -1.9036703983737624 2316 14 -2.1710027911151855 2316 15 -1.9809360222102441 2316 16 -1.8076414276995934 2316 17 -1.9363553620988014 2316 18 -1.8756072105639348 2316 19 -1.8505173655578133 2316 20 -2.1726985557294185 2316 21 -2.0943053607048725 2316 22 -1.9196873762883726 2316 23 -1.8952880566560921 2316 24 -1.7855208685938564 2316 25 -1.8768962219260459 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38615.0 3 1.0 4 773.0 5 6277.0 6 7793.0 7 6107.0 8 4933.0 9 4476.0 10 4011.0 11 3855.0 12 3647.0 13 3607.0 14 3520.0 15 3421.0 16 3658.0 17 3735.0 18 4022.0 19 4502.0 20 5509.0 21 6984.0 22 9038.0 23 11380.0 24 13827.0 25 16562.0 26 18803.0 27 21477.0 28 25273.0 29 30890.0 30 38856.0 31 49238.0 32 62439.0 33 80252.0 34 103062.0 35 134002.0 36 191180.0 37 316196.0 38 531123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.73645772690514 20.240411733125534 14.099469703319281 24.923660836650047 2 14.770530229368251 20.851428390947994 41.188994745759274 23.18904663392448 3 19.019324957530664 24.718393903366188 30.158811625656217 26.10346951344693 4 13.00864501952574 17.642483773668335 37.84688930449555 31.501981902310376 5 13.072263428906385 38.119583571462016 35.96776532143 12.840387678201592 6 32.047371638831294 37.410408314740074 17.820821141494513 12.721398904934114 7 28.474476662733693 31.49718788704623 21.932845434179864 18.095490016040213 8 24.756802425658922 38.38957183239671 20.17693864337264 16.676687098571723 9 26.173292935764707 15.590476040075712 20.33034713182527 37.905883892334316 10 15.195843983567245 29.311624528212498 34.30399922393353 21.18853226428673 11 34.21122092852744 22.07080027342807 25.308283381574288 18.409695416470207 12 23.9948923997374 24.332785875590226 31.72301420607723 19.949307518595138 13 29.291658864641825 22.052244614346854 25.7971037379783 22.85899278303302 14 20.51195571006698 22.92813940319586 27.39001400980461 29.169890876932552 15 22.561425435578585 32.353004211965406 23.008171258017285 22.077399094438714 16 21.626818059788704 28.328230997087495 28.60944229246426 21.435508650659543 17 21.36286521936286 29.418502868513137 28.05694613331649 21.16168577880752 18 21.228858392685122 27.436826158854487 30.880000721922297 20.45431472653809 19 23.787396139069305 26.810840565716358 27.51849361888368 21.88326967633065 20 23.786211735298167 27.540771689817063 28.29016087587223 20.382855699012545 21 22.64766131015361 26.898430044601263 27.172816918249065 23.28109172699606 22 23.251030431280892 28.70041578212385 27.249126361218334 20.799427425376923 23 21.786599768533662 28.470190249085753 28.118478729236273 21.62473125314431 24 23.428916597670447 26.853197100579568 28.752078346617456 20.96580795513253 25 22.519468037222502 27.843382559481437 28.37172416360484 21.26542523969122 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 288.0 1 288.0 2 316.0 3 344.0 4 344.0 5 344.0 6 738.0 7 1132.0 8 1132.0 9 1132.0 10 1681.0 11 2230.0 12 2230.0 13 2230.0 14 3817.5 15 5405.0 16 5405.0 17 5405.0 18 10847.0 19 16289.0 20 16289.0 21 16289.0 22 28865.5 23 41442.0 24 41442.0 25 41442.0 26 63388.5 27 85335.0 28 85335.0 29 85335.0 30 114610.5 31 143886.0 32 143886.0 33 143886.0 34 164544.5 35 185203.0 36 185203.0 37 185203.0 38 197910.0 39 210617.0 40 210617.0 41 210617.0 42 221869.0 43 233121.0 44 233121.0 45 233121.0 46 234562.0 47 236003.0 48 236003.0 49 236003.0 50 223690.5 51 211378.0 52 211378.0 53 211378.0 54 185658.5 55 159939.0 56 159939.0 57 159939.0 58 135436.5 59 110934.0 60 110934.0 61 110934.0 62 89287.0 63 67640.0 64 67640.0 65 67640.0 66 51919.5 67 36199.0 68 36199.0 69 36199.0 70 26153.5 71 16108.0 72 16108.0 73 16108.0 74 11184.5 75 6261.0 76 6261.0 77 6261.0 78 4307.0 79 2353.0 80 2353.0 81 2353.0 82 1521.5 83 690.0 84 690.0 85 690.0 86 441.5 87 193.0 88 193.0 89 193.0 90 119.0 91 45.0 92 45.0 93 45.0 94 27.0 95 9.0 96 9.0 97 9.0 98 4.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40833730014596364 2 0.0 3 0.0 4 0.0 5 0.030625297510947273 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007783224781787706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1773044.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.83323966412816 #Duplication Level Percentage of deduplicated Percentage of total 1 87.35255185714556 65.36874448398957 2 9.055624149738307 13.553233846112672 3 1.8883457676815176 4.239330942049594 4 0.671137635574087 2.008936141221279 5 0.3074626578816468 1.150421338251356 6 0.17027205496115663 0.7645205698207101 7 0.10585276341147268 0.5544913649436694 8 0.07533773383427066 0.45102133534178185 9 0.05705107001029156 0.3842384755657593 >10 0.2738423081862157 3.6714601508641658 >50 0.021183055416858932 1.1211840750904634 >100 0.017041704373004875 2.591696397597813 >500 0.002487876823257316 1.2794232317922418 >1k 0.0016585845488382097 2.2891171015949467 >5k 1.5078041353074634E-4 0.5721805457640406 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5090 0.28707691405289437 No Hit CTGTAGGACGTGGAATATGGCAAGA 5052 0.28493370722892386 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4289 0.24190037021077876 No Hit CTTTAGGACGTGAAATATGGCGAGG 4264 0.24049036572132446 No Hit GTATCAACGCAGAGTACTTTTTTTT 3504 0.19762622924191392 No Hit GGTATCAACGCAGAGTACTTTTTTT 2292 0.12926921159316973 No Hit TATCAACGCAGAGTACTTTTTTTTT 2285 0.1288744103361225 No Hit CTGAAGGACCTGGAATATGGCGAGA 2247 0.126731203512152 No Hit GTCCTACAGTGTGCATTTCTCATTT 2183 0.123121592019149 No Hit CTGTAGGACCTGGAATATGGCGAGA 1828 0.10309952826889801 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.640017957817178E-5 0.0 5 0.0 0.0 0.0 5.640017957817178E-5 0.0 6 0.0 0.0 0.0 5.640017957817178E-5 0.0 7 0.0 0.0 0.0 5.640017957817178E-5 0.0 8 0.0 0.0 0.0 5.640017957817178E-5 0.0 9 0.0 0.0 0.0 1.1280035915634356E-4 0.0 10 0.0 0.0 0.0 1.1280035915634356E-4 0.0 11 0.0 0.0 0.0 1.1280035915634356E-4 0.0 12 0.0 0.0 0.0 1.1280035915634356E-4 5.640017957817178E-5 13 0.0 0.0 0.0 1.1280035915634356E-4 5.640017957817178E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 140 0.0 13.567501 18 TAGGACC 840 0.0 12.327637 4 CCAACGA 105 2.7277383E-7 11.759496 19 TGTAGGA 2850 0.0 11.433571 2 CTGTAGG 2845 0.0 11.196403 1 GGCGAGG 1225 0.0 11.165059 19 TGGACCG 60 0.0058753 11.083564 5 GGACCGT 60 0.005888884 11.080126 6 GACGTGG 1505 0.0 11.043314 7 GTAGGAC 2885 0.0 10.899707 3 AGGACGT 3090 0.0 10.883721 5 AATCCCG 175 2.0008883E-11 10.8549185 19 GGACGTG 3120 0.0 10.775727 6 ATAGGAC 275 0.0 10.709313 3 GTACTAG 100 2.2979959E-5 10.490761 1 CACCTTT 625 0.0 10.484963 14 GCCGGTT 100 2.4065708E-5 10.446976 11 CGGTTTC 110 6.0665243E-6 10.360637 13 ACTGTTC 465 0.0 10.212097 8 ACCTTTT 680 0.0 10.195577 15 >>END_MODULE