FastQCFastQC Report
Thu 26 May 2016
SRR1512168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751925
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79770.2898698183998474No Hit
TATCAACGCAGAGTACTTTTTTTTT48540.17638562097440882No Hit
GGTATCAACGCAGAGTACTTTTTTT47140.17129827302706288No Hit
GTCCTACAGTGGACATTTCTAAATT39430.14328152111703624No Hit
CTGTAGGACGTGGAATATGGCAAGA37150.13499641160278714No Hit
GTCCTAAAGTGTGTATTTCTCATTT27630.10040244556083469No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTA400.005573663414.13436919
TAGGACC6600.012.848264
GCACCGT951.1495595E-611.9015466
TCCAACG3650.011.10096618
AGGCGTA600.00624916410.99247
TGTAGGA21700.010.8275922
TAAACGC700.00159813910.7680654
GTAGGAC22200.010.6104253
GTCCTAC18650.010.5168611
GGCGAGG8250.010.50797719
GATATAC10200.010.315751
AGGACGT22700.010.2521945
GTCTTAC2400.010.2155971
GTCGAGG1201.6927606E-610.20815619
CCAACGT1856.91216E-1110.186933519
CTGTAGG22900.010.1709871
GGACGTG21850.010.1335636
ACTGTTC10600.010.1329728
GACGTGG11950.010.0922457
TTAGGAC12550.010.06020453
Produced by FastQC (version 0.11.5)