FastQCFastQC Report
Thu 26 May 2016
SRR1512168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751925
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106170.3858026654069424No Hit
TATCAACGCAGAGTACTTTTTTTTT71190.2586916431225415No Hit
GGTATCAACGCAGAGTACTTTTTTT66360.24114029270419796No Hit
GTCCTACAGTGGACATTTCTAAATT44430.16145062092898607No Hit
CTGTAGGACGTGGAATATGGCAAGA41450.15062183744106397No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35260.12812849187387013No Hit
GTCCTAAAGTGTGTATTTCTCATTT31160.11322983002807126No Hit
CTTTAGGACGTGAAATATGGCGAGG29240.10625289570028254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2500.014.81716519
TCCAACG4350.013.53616418
TAGGACC7450.012.7524834
CGCCCTA701.0945536E-412.21061816
CGGTTTC2650.011.82663613
GCCGGTT2700.011.60762511
CCGGTTT2800.011.19306712
AGGACGT19800.010.70017055
TGCCGGT2850.010.66346410
GGACGTG19500.010.4225116
CGTTTCC2750.010.36052414
CCAACGT2400.010.28969919
GTAGGAC20950.010.2035083
TAGGACG21350.010.145844
CTAGGAC3750.010.1339733
CGTTGTT750.002655286810.1302914
TGTAGGA22100.010.10244752
CTGTAGG21450.010.0924021
GGACCGT951.6498598E-49.9969986
ATAGGAC3150.09.953013
Produced by FastQC (version 0.11.5)