##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512168_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2751925 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.001115582728453 31.0 31.0 34.0 28.0 34.0 2 31.18965814838704 31.0 31.0 34.0 28.0 34.0 3 31.27999709294403 31.0 31.0 34.0 28.0 34.0 4 34.878719078463256 37.0 35.0 37.0 32.0 37.0 5 34.509661418825004 37.0 35.0 37.0 31.0 37.0 6 34.45159043215204 37.0 35.0 37.0 30.0 37.0 7 34.28714917739401 37.0 35.0 37.0 30.0 37.0 8 34.198281929921784 37.0 35.0 37.0 30.0 37.0 9 35.707972055924486 39.0 35.0 39.0 30.0 39.0 10 35.535266041044 39.0 35.0 39.0 30.0 39.0 11 35.522396867647196 39.0 35.0 39.0 30.0 39.0 12 35.34255911770851 39.0 35.0 39.0 30.0 39.0 13 35.32031468880874 39.0 35.0 39.0 30.0 39.0 14 36.34642041480055 40.0 36.0 41.0 29.0 41.0 15 36.270334038900046 40.0 36.0 41.0 29.0 41.0 16 36.170388727890476 39.0 36.0 41.0 29.0 41.0 17 36.12211306630813 39.0 36.0 41.0 29.0 41.0 18 36.05979450748113 39.0 36.0 41.0 28.0 41.0 19 36.092301934100675 39.0 36.0 41.0 28.0 41.0 20 35.96861724065881 39.0 36.0 41.0 27.0 41.0 21 35.86983402527322 39.0 36.0 41.0 27.0 41.0 22 35.9628401936826 39.0 36.0 41.0 28.0 41.0 23 35.864643840220936 39.0 36.0 41.0 27.0 41.0 24 35.7346566494363 39.0 36.0 41.0 27.0 41.0 25 35.28362727908646 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12926819830292402 1101 2 0.19209028053283106 1101 3 0.16591310995299224 1101 4 0.23985181288517765 1101 5 0.23950112958201686 1101 6 0.21992729946806833 1101 7 0.2462564413614743 1101 8 -0.011931781725714075 1101 9 0.08871032324870498 1101 10 0.12985029671393988 1101 11 0.18383585646538592 1101 12 0.1528484633955287 1101 13 0.060032559335724045 1101 14 -0.06561815260108261 1101 15 0.020844263900819726 1101 16 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0.42407736245866445 1103 3 0.3515695931186613 1103 4 0.5026446040058588 1103 5 0.46030136344370476 1103 6 0.5378667490888134 1103 7 0.3559111241633559 1103 8 0.43363892122044234 1103 9 0.5809910315155307 1103 10 0.5836565812827814 1103 11 0.4682093484287151 1103 12 0.6306317577519138 1103 13 0.6371654645413116 1103 14 0.5548727404041003 1103 15 0.7961546874503185 1103 16 0.7776638032261758 1103 17 0.6577278987323254 1103 18 0.6792128904702466 1103 19 0.7888151052164929 1103 20 0.566098305056137 1103 21 0.7361811342795122 1103 22 0.8795471203065048 1103 23 0.9675641831647042 1103 24 0.5981969865925976 1103 25 0.7583341217551336 1104 1 0.3397204913460641 1104 2 0.3140903776956776 1104 3 0.33016204116169945 1104 4 0.24985958591277324 1104 5 0.3383983312920833 1104 6 0.45180399981785513 1104 7 0.2663715793327128 1104 8 0.28627313471423577 1104 9 0.5822271324208828 1104 10 0.4288519484823041 1104 11 0.47812025211248965 1104 12 0.5831778454398346 1104 13 0.541489030381193 1104 14 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-0.8515761744621315 2314 16 -0.8273425026960837 2314 17 -0.9465741906520009 2314 18 -0.8838562714715792 2314 19 -0.7584745261981141 2314 20 -0.8293702658297164 2314 21 -0.9137455727185468 2314 22 -0.7874136754839469 2314 23 -0.794272949551214 2314 24 -0.7742661957299077 2314 25 -1.02029855698445 2315 1 -0.46753148577302284 2315 2 -0.5160814807173395 2315 3 -0.5180592915980995 2315 4 -0.5332389069778856 2315 5 -0.561299378643632 2315 6 -0.729510720248129 2315 7 -0.8261072041273678 2315 8 -0.6862229337390744 2315 9 -0.6816863252091778 2315 10 -0.650926015321815 2315 11 -0.6867722445035156 2315 12 -0.6777017357739794 2315 13 -0.8321956033387465 2315 14 -0.9541462265209759 2315 15 -0.9218035019138711 2315 16 -1.0342463818923449 2315 17 -0.9356835256484288 2315 18 -1.0351700521754168 2315 19 -1.1482120047677 2315 20 -1.1006912387292687 2315 21 -1.133507806943129 2315 22 -0.9454237215060388 2315 23 -1.1915302752908303 2315 24 -0.8900278549141305 2315 25 -0.9177541291443632 2316 1 -0.8173856057934721 2316 2 -0.7763245741586751 2316 3 -0.7516669043465427 2316 4 -0.7732648544824485 2316 5 -0.8369209379622475 2316 6 -0.9795653825355473 2316 7 -0.9057407618180733 2316 8 -0.8743329467170966 2316 9 -1.1333046492036587 2316 10 -1.1371298731981625 2316 11 -1.1124184018514711 2316 12 -1.1885839082241034 2316 13 -1.0837240121409124 2316 14 -1.3620090976319972 2316 15 -1.3029765503314223 2316 16 -1.212704871223167 2316 17 -1.3396264061203667 2316 18 -1.3652940757809802 2316 19 -1.2565964309695516 2316 20 -1.3192476119889847 2316 21 -1.182892301045797 2316 22 -1.2314826816158089 2316 23 -1.1744900545235168 2316 24 -1.2085341139595656 2316 25 -1.3053267221567282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36035.0 3 3.0 4 642.0 5 5978.0 6 7766.0 7 6148.0 8 5246.0 9 4709.0 10 4448.0 11 4267.0 12 4156.0 13 3867.0 14 4043.0 15 3892.0 16 4035.0 17 4224.0 18 4626.0 19 5171.0 20 6378.0 21 8064.0 22 10804.0 23 13434.0 24 16716.0 25 20097.0 26 23385.0 27 27113.0 28 31561.0 29 39065.0 30 50226.0 31 64304.0 32 83626.0 33 109047.0 34 142593.0 35 187843.0 36 272166.0 37 468631.0 38 1067616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.83583136344495 19.631436864195567 14.181809689442446 24.350922082917034 2 14.633211297546264 20.525668395759332 41.27972237615488 23.56139793053953 3 18.83528802565477 24.596963943421425 29.99525786494908 26.572490165974727 4 12.4813721304178 17.259990733759096 38.044278096241726 32.21435903958139 5 13.11379648524882 37.8791029118506 35.64914137738756 13.357959225513024 6 31.634401373583948 37.46214013826685 17.903721940096478 12.999736548052725 7 27.931902213904813 31.85675481708259 21.884935090890924 18.326407878121678 8 24.878366961308902 37.675590722857635 20.217447786549418 17.228594529284045 9 25.872398412020676 16.061266204565893 20.4547725682931 37.61156281512032 10 14.864612952751255 29.816837304795733 33.83951234136105 21.479037401091965 11 34.251296819499075 23.0100384276461 24.049892348083617 18.688772404771207 12 22.973700228022203 25.007149540776002 31.663290242284948 20.35585998891685 13 28.52396050037701 22.018986709303487 26.31358049365444 23.143472296665063 14 20.87422440655178 22.36510806072113 27.830009902159397 28.93065763056769 15 22.918829546590114 30.835433378453263 23.870236289143055 22.375500785813568 16 21.865094433896274 28.78432370068225 27.742325826466928 21.60825603895455 17 21.277651098776314 28.85772686392253 28.08397757933083 21.78064445797033 18 21.015180282892885 28.018895863804428 30.14729689217548 20.81862696112721 19 22.84542638334984 27.33839039944766 28.150658175640686 21.665525041561814 20 22.825186006159328 27.661218964906382 28.798313907537448 20.71528112139684 21 22.793971492682395 27.0620383913079 27.8687827611581 22.275207354851602 22 22.40686065208899 27.895273308683922 28.361601424457426 21.33626461476966 23 22.25122414309983 27.522079998546474 28.29001517119834 21.936680687155356 24 22.38149658875151 27.044341688091063 28.97208317813894 21.60207854501849 25 22.42541216009466 27.42857018259839 28.685165143862644 21.46085251344431 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 348.0 1 348.0 2 600.5 3 853.0 4 853.0 5 853.0 6 2214.5 7 3576.0 8 3576.0 9 3576.0 10 5265.0 11 6954.0 12 6954.0 13 6954.0 14 10638.0 15 14322.0 16 14322.0 17 14322.0 18 24795.5 19 35269.0 20 35269.0 21 35269.0 22 55331.5 23 75394.0 24 75394.0 25 75394.0 26 107274.0 27 139154.0 28 139154.0 29 139154.0 30 177984.5 31 216815.0 32 216815.0 33 216815.0 34 241765.5 35 266716.0 36 266716.0 37 266716.0 38 286654.0 39 306592.0 40 306592.0 41 306592.0 42 327169.5 43 347747.0 44 347747.0 45 347747.0 46 350683.0 47 353619.0 48 353619.0 49 353619.0 50 341383.0 51 329147.0 52 329147.0 53 329147.0 54 292250.0 55 255353.0 56 255353.0 57 255353.0 58 218489.0 59 181625.0 60 181625.0 61 181625.0 62 147930.5 63 114236.0 64 114236.0 65 114236.0 66 87587.5 67 60939.0 68 60939.0 69 60939.0 70 44268.5 71 27598.0 72 27598.0 73 27598.0 74 19115.0 75 10632.0 76 10632.0 77 10632.0 78 7126.5 79 3621.0 80 3621.0 81 3621.0 82 2321.0 83 1021.0 84 1021.0 85 1021.0 86 667.0 87 313.0 88 313.0 89 313.0 90 190.5 91 68.0 92 68.0 93 68.0 94 40.0 95 12.0 96 12.0 97 12.0 98 6.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41785295747522194 2 0.0 3 0.0 4 0.0 5 0.03237733586489457 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007376654523651625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2751925.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.30183574900708 #Duplication Level Percentage of deduplicated Percentage of total 1 83.88703940802539 59.812999053415105 2 11.034957385635835 15.736254380157977 3 2.6534771136123667 5.6759336795561515 4 0.9728769558662078 2.7747165164466545 5 0.4626927475816622 1.6495421145162232 6 0.242743148605017 1.0384819266619045 7 0.15411933793162497 0.7692294203262509 8 0.11086503895217506 0.6323904638140208 9 0.07583765373540638 0.48666275372088175 >10 0.3657009190187487 4.461776129874203 >50 0.0214527170002109 1.0386574524341168 >100 0.014621491590140371 2.061230914330748 >500 0.0019353680694157538 0.9526033183696025 >1k 0.0015279221598904838 2.02446362411648 >5k 1.0186147732603226E-4 0.49950665763295865 >10k+ 5.093073866301613E-5 0.38555159462661737 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 10617 0.3858026654069424 No Hit TATCAACGCAGAGTACTTTTTTTTT 7119 0.2586916431225415 No Hit GGTATCAACGCAGAGTACTTTTTTT 6636 0.24114029270419796 No Hit GTCCTACAGTGGACATTTCTAAATT 4443 0.16145062092898607 No Hit CTGTAGGACGTGGAATATGGCAAGA 4145 0.15062183744106397 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3526 0.12812849187387013 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3116 0.11322983002807126 No Hit CTTTAGGACGTGAAATATGGCGAGG 2924 0.10625289570028254 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 3.6338199623899635E-5 11 3.6338199623899635E-5 0.0 0.0 0.0 3.6338199623899635E-5 12 7.267639924779927E-5 0.0 0.0 0.0 7.267639924779927E-5 13 7.267639924779927E-5 0.0 0.0 0.0 7.267639924779927E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 250 0.0 14.817165 19 TCCAACG 435 0.0 13.536164 18 TAGGACC 745 0.0 12.752483 4 CGCCCTA 70 1.0945536E-4 12.210618 16 CGGTTTC 265 0.0 11.826636 13 GCCGGTT 270 0.0 11.607625 11 CCGGTTT 280 0.0 11.193067 12 AGGACGT 1980 0.0 10.7001705 5 TGCCGGT 285 0.0 10.663464 10 GGACGTG 1950 0.0 10.422511 6 CGTTTCC 275 0.0 10.360524 14 CCAACGT 240 0.0 10.289699 19 GTAGGAC 2095 0.0 10.203508 3 TAGGACG 2135 0.0 10.14584 4 CTAGGAC 375 0.0 10.133973 3 CGTTGTT 75 0.0026552868 10.13029 14 TGTAGGA 2210 0.0 10.1024475 2 CTGTAGG 2145 0.0 10.092402 1 GGACCGT 95 1.6498598E-4 9.996998 6 ATAGGAC 315 0.0 9.95301 3 >>END_MODULE