FastQCFastQC Report
Thu 26 May 2016
SRR1512167_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512167_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361526
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT64700.27397538710139124No Hit
CTGTAGGACGTGGAATATGGCAAGA56380.23874393083116596No Hit
GTATCAACGCAGAGTACTTTTTTTT55510.2350598723029092No Hit
GTCCTAAAGTGTGTATTTCTCATTT48880.20698480558757346No Hit
CTTTAGGACGTGAAATATGGCGAGG46530.1970336130112478No Hit
TATCAACGCAGAGTACTTTTTTTTT33760.142958409096491No Hit
GGTATCAACGCAGAGTACTTTTTTT32990.13969780557148218No Hit
GTCCTACAGTGTGCATTTCTCATTT26050.11031002834607792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCAA453.778677E-516.76093317
CCAACGA2300.015.16682419
ATACCGT602.7619932E-514.1411396
TCCAACG4000.013.67092518
CCGGTTT2650.013.1633812
GCCGGTT2850.012.56963611
TAGGACC9250.012.4340114
GGTATCA14450.012.1676751
GGCGAGG15050.012.0277819
CGTAGGA708.4845297E-411.6150572
GGCCTAC2300.011.0469281
GATATAC10400.010.9449571
CGGTTTC3200.010.90092413
GGTCTAA1508.556526E-910.8407191
CGGACTG700.001590422810.7742015
ACTGTTC11150.010.7377398
AAGTCGT1855.456968E-1210.7014047
GTCCTAC29350.010.6999011
TGCCGGT3350.010.6935710
AGGACGT35350.010.5608515
Produced by FastQC (version 0.11.5)