FastQCFastQC Report
Thu 26 May 2016
SRR1512167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361526
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69820.29565628326768373No Hit
GTCCTACAGTGGACATTTCTAAATT68430.28977025872253787No Hit
CTGTAGGACGTGGAATATGGCAAGA60740.2572065689727744No Hit
CTTTAGGACGTGAAATATGGCGAGG54550.23099470427172938No Hit
GTCCTAAAGTGTGTATTTCTCATTT51660.21875685467786504No Hit
TATCAACGCAGAGTACTTTTTTTTT45870.19423880998981166No Hit
GGTATCAACGCAGAGTACTTTTTTT45210.19144400696837555No Hit
GTCCTACAGTGTGCATTTCTCATTT28880.12229380493799348No Hit
CTGAAGGACCTGGAATATGGCGAGA27410.11606901639024936No Hit
TTGTAGAACAGTGTATATCAATGAG23890.10116340027592327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG402.7613383E-416.6254275
CTGATCG551.9656935E-413.8141479
CCAACGA2550.013.78145719
TCCAACG3600.013.19059318
TAGGACC9700.011.948764
GGTATCA15350.011.7428821
GGCGAGG14950.011.5628219
AGGACGT32400.011.3181925
TGTCGAG2400.011.08009718
GCCGGTT2750.011.05131811
GGACGTG32200.011.030946
TGTAGGA32050.010.9971622
TTAGGAC19900.010.9324413
GTAGGAC30850.010.9322093
TAGGACG35000.010.77599054
GAACAGT6700.010.7729736
CCGGTTT2650.010.75157712
GACGTGA17450.010.7217977
GACGTGG16250.010.6953387
CTGTAGG31400.010.6913931
Produced by FastQC (version 0.11.5)