##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512167_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2361526 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.93134058231838 31.0 31.0 34.0 28.0 34.0 2 31.141163806792726 31.0 31.0 34.0 28.0 34.0 3 31.232197739935955 31.0 31.0 34.0 27.0 34.0 4 34.822459714608264 37.0 35.0 37.0 32.0 37.0 5 34.44087721244653 37.0 35.0 37.0 30.0 37.0 6 34.382900294131844 37.0 35.0 37.0 30.0 37.0 7 34.21263411878591 37.0 35.0 37.0 30.0 37.0 8 34.12129529804034 37.0 35.0 37.0 30.0 37.0 9 35.6259850622013 39.0 35.0 39.0 30.0 39.0 10 35.43263677808332 39.0 35.0 39.0 30.0 39.0 11 35.414631471345224 39.0 35.0 39.0 30.0 39.0 12 35.24360265353843 39.0 35.0 39.0 29.0 39.0 13 35.22491262006008 39.0 35.0 39.0 29.0 39.0 14 36.250552397051734 39.0 36.0 41.0 29.0 41.0 15 36.18303715478889 39.0 36.0 41.0 29.0 41.0 16 36.08580807494815 39.0 36.0 41.0 28.0 41.0 17 36.02438169217701 39.0 36.0 41.0 27.0 41.0 18 35.96679646974033 39.0 36.0 41.0 27.0 41.0 19 35.99051714865727 39.0 36.0 41.0 27.0 41.0 20 35.869521233304226 39.0 36.0 41.0 27.0 41.0 21 35.757325559828686 39.0 36.0 41.0 27.0 41.0 22 35.85132029035463 39.0 36.0 41.0 27.0 41.0 23 35.741648408698445 39.0 36.0 41.0 27.0 41.0 24 35.615472368290675 39.0 36.0 41.0 27.0 41.0 25 35.18380953671482 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2981796812475537 1101 2 0.2835273477925071 1101 3 0.3932361928673558 1101 4 0.39631942131470765 1101 5 0.39585213094437677 1101 6 0.3749096700696768 1101 7 0.28295108224898513 1101 8 0.1411125108512863 1101 9 0.3017398626075405 1101 10 0.40346430461191574 1101 11 0.3664596797013786 1101 12 0.4390761795770075 1101 13 0.4193354312338542 1101 14 0.2926587112720185 1101 15 0.38589522748682725 1101 16 0.4748228189524255 1101 17 0.3486099614100553 1101 18 0.42807447092992135 1101 19 0.4337185924536584 1101 20 0.345705415673784 1101 21 0.331048565077424 1101 22 0.30017240740442475 1101 23 0.19131144015773316 1101 24 0.026704580863452065 1101 25 0.3118633316213888 1102 1 0.37618581361943626 1102 2 0.29377656453352685 1102 3 0.21806732237954662 1102 4 0.2776889249461476 1102 5 0.40674125058950494 1102 6 0.4212292813295875 1102 7 0.3601067342665871 1102 8 0.3121805061985299 1102 9 0.44209288994620977 1102 10 0.34146762928445895 1102 11 0.383742628944411 1102 12 0.5184716774534124 1102 13 0.47442056097482066 1102 14 0.6348106716849387 1102 15 0.5852604497481693 1102 16 0.6877882991061455 1102 17 0.6988975648975426 1102 18 0.7329479090543174 1102 19 0.6620981934038213 1102 20 0.7908776583719046 1102 21 0.6292225206206936 1102 22 0.4779863089329126 1102 23 0.5080604065827004 1102 24 0.5352981463296445 1102 25 0.5245265012911062 1103 1 0.4486808781828948 1103 2 0.31342862431299423 1103 3 0.35875877041550197 1103 4 0.4334686308320812 1103 5 0.3520866165779921 1103 6 0.45669604079750314 1103 7 0.3358088838040487 1103 8 0.4469437019124882 1103 9 0.5669699963232162 1103 10 0.3815783892879807 1103 11 0.24527866139047916 1103 12 0.4938288655757077 1103 13 0.533960149369797 1103 14 0.44348203856277024 1103 15 0.48317227854133193 1103 16 0.639873570274105 1103 17 0.44910199973725184 1103 18 0.6862714252189903 1103 19 0.8253059371520877 1103 20 0.40352465633042556 1103 21 0.7339167884969271 1103 22 0.7557468416235125 1103 23 0.9326312903355358 1103 24 0.7617713123772774 1103 25 0.9772204225947903 1104 1 0.2934574938651373 1104 2 0.21898451573430933 1104 3 0.33308317643690444 1104 4 0.20385736186405978 1104 5 0.41362461307155485 1104 6 0.32588170067295863 1104 7 0.4977761167403898 1104 8 0.3902784458820179 1104 9 0.6986669916681194 1104 10 0.5593215728923582 1104 11 0.5392240093138199 1104 12 0.6855705982399982 1104 13 0.7151462928919514 1104 14 0.7947909074340629 1104 15 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-0.6471495050945819 2314 15 -0.4970449227930942 2314 16 -0.5679351254736531 2314 17 -0.6460994555996251 2314 18 -0.6599783892915099 2314 19 -0.710176143188221 2314 20 -0.729349029442119 2314 21 -0.8481300263048581 2314 22 -0.5088079011368976 2314 23 -0.603587252019274 2314 24 -0.6062783273608972 2314 25 -0.602210303259973 2315 1 -0.10394269219281682 2315 2 -0.13123191118412336 2315 3 -0.17681249271822352 2315 4 -0.20624281727438643 2315 5 0.04283263662864556 2315 6 -0.15479287473817038 2315 7 -0.18610372980023726 2315 8 0.01862460593798687 2315 9 -0.09401908550749027 2315 10 -0.12507070185834124 2315 11 -0.16743909508621613 2315 12 -0.11561417576109534 2315 13 -0.10372013748624198 2315 14 -0.24968232559199066 2315 15 -0.38212932335029137 2315 16 -0.4666516610773286 2315 17 -0.34479495309496855 2315 18 -0.4430733955714885 2315 19 -0.5357691767087118 2315 20 -0.4818101735937006 2315 21 -0.4248146301139428 2315 22 -0.4561542687781923 2315 23 -0.5077411950570223 2315 24 -0.3060631235882951 2315 25 -0.27126443410302414 2316 1 -0.8699618273517231 2316 2 -0.8622577486132883 2316 3 -0.7978398904642745 2316 4 -0.7991359284753301 2316 5 -0.9726531538491727 2316 6 -0.9386613258399663 2316 7 -1.0031423901972687 2316 8 -1.036351741076487 2316 9 -1.3138273083774834 2316 10 -1.3972350032264558 2316 11 -1.4685377000210735 2316 12 -1.5536646307353124 2316 13 -1.4625880566618221 2316 14 -1.7505905009432396 2316 15 -1.7048095931432599 2316 16 -1.6765291762017682 2316 17 -1.6876703334222114 2316 18 -1.8511409431079642 2316 19 -1.7419863302651706 2316 20 -1.8590947966707887 2316 21 -1.7947278197562753 2316 22 -1.7120129040683025 2316 23 -1.7420851881916306 2316 24 -1.6149792707403634 2316 25 -1.7342327951319874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32862.0 3 2.0 4 579.0 5 5381.0 6 7057.0 7 5683.0 8 4588.0 9 4266.0 10 3993.0 11 3928.0 12 3642.0 13 3653.0 14 3469.0 15 3463.0 16 3549.0 17 3642.0 18 4187.0 19 4744.0 20 5608.0 21 7319.0 22 9479.0 23 12242.0 24 14781.0 25 17827.0 26 20759.0 27 23524.0 28 27833.0 29 33711.0 30 43341.0 31 55743.0 32 72184.0 33 93205.0 34 121333.0 35 161305.0 36 232101.0 37 400852.0 38 909691.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.94286958385353 20.10003946128542 14.364205552475005 24.59288540238605 2 14.16084345461367 20.648131758871173 42.11823202454684 23.07279276196832 3 18.62359338834296 24.879717606327432 30.337967907192215 26.158721098137388 4 12.461857290582445 17.593962547945694 38.49320312374287 31.450977037728993 5 12.838133208175687 38.08762955595581 36.142786463319375 12.931450772549132 6 31.324236955256897 37.848704608799565 18.031603293802398 12.79545514214114 7 27.709625047532825 31.771320747686033 22.271531204822644 18.247522999958502 8 24.31919021852819 38.70704790038306 20.42729997467739 16.546461906411363 9 25.890038898576602 15.80003777218629 20.783679705410822 37.52624362382629 10 14.987808730456493 29.694485684256705 34.37209668663398 20.94560889865282 11 33.851289378139384 22.548301394945472 25.2699737373207 18.33043548959444 12 23.403722847006556 24.658462367130408 32.19731648095342 19.74049830490962 13 28.825598363092343 22.399880416307084 26.214024321561567 22.560496899039013 14 20.30123742021049 23.08270160904432 27.81320214132726 28.802858829417925 15 22.220081421928025 32.36847699326622 23.574841013819032 21.836600570986725 16 21.176010765920005 28.769321193160696 28.92930249338775 21.125365547531555 17 20.83296986778888 29.507276227320812 28.598626481351463 21.061127423538846 18 20.68929158518687 27.962639411973445 31.230695745039437 20.117373257800253 19 23.1320341169227 27.10031564335942 28.07320351332147 21.694446726396407 20 23.041330055227004 28.004053311291088 28.861888456870684 20.092728176611224 21 22.252518075176813 27.290616321819027 27.702934458481508 22.753931144522653 22 22.498545432063842 28.812725330993604 28.142565442853478 20.546163794089075 23 21.651211970564795 28.460664841293298 28.5218117437623 21.36631144437961 24 22.77281723766751 27.17564828843722 29.315578147350486 20.735956326544784 25 22.190756664091825 27.855446463660883 29.007475505142903 20.94632136710439 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 264.0 1 264.0 2 432.5 3 601.0 4 601.0 5 601.0 6 1581.0 7 2561.0 8 2561.0 9 2561.0 10 3761.0 11 4961.0 12 4961.0 13 4961.0 14 8105.0 15 11249.0 16 11249.0 17 11249.0 18 20260.5 19 29272.0 20 29272.0 21 29272.0 22 47665.5 23 66059.0 24 66059.0 25 66059.0 26 96587.0 27 127115.0 28 127115.0 29 127115.0 30 166711.0 31 206307.0 32 206307.0 33 206307.0 34 227522.0 35 248737.0 36 248737.0 37 248737.0 38 262427.5 39 276118.0 40 276118.0 41 276118.0 42 289521.0 43 302924.0 44 302924.0 45 302924.0 46 303106.5 47 303289.0 48 303289.0 49 303289.0 50 287927.0 51 272565.0 52 272565.0 53 272565.0 54 237759.0 55 202953.0 56 202953.0 57 202953.0 58 172057.0 59 141161.0 60 141161.0 61 141161.0 62 114034.5 63 86908.0 64 86908.0 65 86908.0 66 66269.0 67 45630.0 68 45630.0 69 45630.0 70 33298.0 71 20966.0 72 20966.0 73 20966.0 74 14472.5 75 7979.0 76 7979.0 77 7979.0 78 5390.5 79 2802.0 80 2802.0 81 2802.0 82 1830.0 83 858.0 84 858.0 85 858.0 86 523.0 87 188.0 88 188.0 89 188.0 90 118.5 91 49.0 92 49.0 93 49.0 94 28.0 95 7.0 96 7.0 97 7.0 98 5.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4172725602004806 2 0.0 3 0.0 4 0.0 5 0.03260603525008829 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.009061937069505057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2361526.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.47747314706312 #Duplication Level Percentage of deduplicated Percentage of total 1 86.4045084373909 62.62380440056188 2 9.561082490515705 13.859261989264148 3 2.0709318671807666 4.502877263789739 4 0.7121561072932355 2.0646110057144993 5 0.3609208292702033 1.3079314855823456 6 0.19282539193461756 0.8385298299607901 7 0.13752857031602733 0.6977406283423698 8 0.10588913006360165 0.613966126460045 9 0.07665872100323834 0.500042735370037 >10 0.333007841476666 4.178592766575613 >50 0.021872787364057726 1.101196381392626 >100 0.018122916539240534 2.727904565180963 >500 0.0020431407508204663 1.019098114211943 >1k 0.002159891650864652 2.6731704675583234 >5k 2.9187725011684484E-4 1.2912722400348207 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6982 0.29565628326768373 No Hit GTCCTACAGTGGACATTTCTAAATT 6843 0.28977025872253787 No Hit CTGTAGGACGTGGAATATGGCAAGA 6074 0.2572065689727744 No Hit CTTTAGGACGTGAAATATGGCGAGG 5455 0.23099470427172938 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5166 0.21875685467786504 No Hit TATCAACGCAGAGTACTTTTTTTTT 4587 0.19423880998981166 No Hit GGTATCAACGCAGAGTACTTTTTTT 4521 0.19144400696837555 No Hit GTCCTACAGTGTGCATTTCTCATTT 2888 0.12229380493799348 No Hit CTGAAGGACCTGGAATATGGCGAGA 2741 0.11606901639024936 No Hit TTGTAGAACAGTGTATATCAATGAG 2389 0.10116340027592327 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.469100064957998E-5 2 0.0 0.0 0.0 0.0 8.469100064957998E-5 3 0.0 0.0 0.0 4.234550032478999E-5 8.469100064957998E-5 4 0.0 0.0 0.0 4.234550032478999E-5 8.469100064957998E-5 5 0.0 4.234550032478999E-5 0.0 4.234550032478999E-5 8.469100064957998E-5 6 0.0 4.234550032478999E-5 0.0 4.234550032478999E-5 8.469100064957998E-5 7 0.0 4.234550032478999E-5 0.0 4.234550032478999E-5 8.469100064957998E-5 8 0.0 4.234550032478999E-5 0.0 4.234550032478999E-5 8.469100064957998E-5 9 0.0 4.234550032478999E-5 0.0 4.234550032478999E-5 8.469100064957998E-5 10 0.0 8.469100064957998E-5 0.0 4.234550032478999E-5 8.469100064957998E-5 11 0.0 8.469100064957998E-5 0.0 4.234550032478999E-5 8.469100064957998E-5 12 0.0 1.2703650097436997E-4 0.0 1.2703650097436997E-4 1.6938200129915996E-4 13 0.0 2.5407300194873995E-4 0.0 1.2703650097436997E-4 1.6938200129915996E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATACCG 40 2.7613383E-4 16.625427 5 CTGATCG 55 1.9656935E-4 13.814147 9 CCAACGA 255 0.0 13.781457 19 TCCAACG 360 0.0 13.190593 18 TAGGACC 970 0.0 11.94876 4 GGTATCA 1535 0.0 11.742882 1 GGCGAGG 1495 0.0 11.56282 19 AGGACGT 3240 0.0 11.318192 5 TGTCGAG 240 0.0 11.080097 18 GCCGGTT 275 0.0 11.051318 11 GGACGTG 3220 0.0 11.03094 6 TGTAGGA 3205 0.0 10.997162 2 TTAGGAC 1990 0.0 10.932441 3 GTAGGAC 3085 0.0 10.932209 3 TAGGACG 3500 0.0 10.7759905 4 GAACAGT 670 0.0 10.772973 6 CCGGTTT 265 0.0 10.751577 12 GACGTGA 1745 0.0 10.721797 7 GACGTGG 1625 0.0 10.695338 7 CTGTAGG 3140 0.0 10.691393 1 >>END_MODULE