FastQCFastQC Report
Thu 26 May 2016
SRR1512166_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512166_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161651
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51900.24009426128454595No Hit
TATCAACGCAGAGTACTTTTTTTTT32770.15159708944690886No Hit
GGTATCAACGCAGAGTACTTTTTTT32540.15053308790364403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14350.012.3622311
CCCGAAC701.1692282E-412.1243163
TAGGACC3900.012.0897734
GTCTTAC1703.6379788E-1211.9259171
TCCAACG1157.8322046E-811.481091518
CTAATAC1850.011.2140823
GATATAC3950.011.0352471
CCTACAC2600.010.8807963
GTCCTAC10800.010.7940591
TCCTACA11750.010.6115282
CTACACT2700.010.4778044
GTATTAG1551.48447725E-810.464031
GTCCTAA6600.010.2906211
TGTAGGA11300.010.2267372
CCTTATA1501.028784E-710.137032
GTTCCGG906.141406E-410.137031
TTACACG750.002815727410.0586924
AGGACGT11450.09.9653535
TAGGACG11700.09.6718194
GGCGAGG4550.09.53453319
Produced by FastQC (version 0.11.5)