FastQCFastQC Report
Thu 26 May 2016
SRR1512166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161651
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70340.32539942849238845No Hit
TATCAACGCAGAGTACTTTTTTTTT49800.23037946458517125No Hit
GGTATCAACGCAGAGTACTTTTTTT45500.21048726181978494No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24250.11218277140944584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.012.3195721
AATCCCG2500.011.77744719
CCGTGCG600.005888432311.080429
AAGCGTG600.005888432311.080427
TAGGACC4050.011.0253624
TGTAGGA12200.010.9801772
TAGACCG700.001491053610.8578035
GTAGGAC11750.010.4304233
TAGGACG11450.010.3718114
GGACGTG11350.010.2924476
TTAGGCA1303.8396684E-710.2313934
GATATAC5600.010.2132211
TTAGGAC7600.010.1256163
GACGTGG5650.010.0858437
TACACCG856.591471E-410.0594365
TAGGACT3700.010.0141244
GTACCGA951.6488673E-49.9973716
AGGACCG1151.025991E-59.9136485
AGGACGT11850.09.861365
AAGTCGT1451.6238482E-79.8250037
Produced by FastQC (version 0.11.5)