##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512166_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2161651 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.711389118780044 31.0 30.0 34.0 26.0 34.0 2 29.913021112103664 31.0 30.0 34.0 26.0 34.0 3 29.97257975501133 31.0 30.0 34.0 26.0 34.0 4 33.588437726534025 35.0 35.0 37.0 28.0 37.0 5 33.059444841003476 35.0 33.0 37.0 26.0 37.0 6 32.96109594009394 35.0 33.0 37.0 26.0 37.0 7 32.71449461545828 35.0 33.0 37.0 25.0 37.0 8 32.57908469035936 35.0 33.0 37.0 25.0 37.0 9 33.89822917760545 37.0 33.0 39.0 25.0 39.0 10 33.63282370743473 37.0 33.0 39.0 25.0 39.0 11 33.633952936898694 37.0 33.0 39.0 24.0 39.0 12 33.378678611857325 37.0 33.0 39.0 22.0 39.0 13 33.33600706126937 37.0 33.0 39.0 22.0 39.0 14 34.12350559826725 38.0 33.0 40.0 19.0 41.0 15 34.000238243823816 38.0 33.0 40.0 19.0 41.0 16 33.87031625364131 38.0 33.0 40.0 18.0 41.0 17 33.752315706837045 38.0 33.0 40.0 17.0 41.0 18 33.652275043473715 38.0 33.0 40.0 17.0 41.0 19 33.65946353042189 38.0 33.0 40.0 18.0 41.0 20 33.481501407951605 38.0 32.0 40.0 17.0 41.0 21 33.3381766991989 38.0 32.0 40.0 16.0 41.0 22 33.40980250743529 38.0 33.0 40.0 11.0 41.0 23 33.261107366545296 38.0 33.0 40.0 10.0 41.0 24 33.06374386984763 38.0 32.0 40.0 2.0 41.0 25 32.50713783122252 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20422691290609407 1101 2 0.2017564654745918 1101 3 0.3407496544901818 1101 4 0.33313451527578763 1101 5 0.29329982400508925 1101 6 0.3151161385411356 1101 7 0.27718774835826565 1101 8 -0.06415597144668084 1101 9 0.2552689037792746 1101 10 0.3824922253058034 1101 11 0.17597108917619408 1101 12 0.3202388393981792 1101 13 0.14883437686562928 1101 14 0.2767360699185275 1101 15 0.14155964315654046 1101 16 0.24540831871417623 1101 17 0.2238132492010365 1101 18 0.46170273407747686 1101 19 0.38100483946656993 1101 20 0.28894019963507844 1101 21 0.4840099454786113 1101 22 0.22537528181315736 1101 23 0.1204257609820516 1101 24 -0.058021437656748276 1101 25 -0.14638193723822468 1102 1 0.6805866939813434 1102 2 0.704508109959832 1102 3 0.6123710234497821 1102 4 0.7912502836370834 1102 5 0.920189282302303 1102 6 1.0997051447111446 1102 7 0.9050322403950517 1102 8 1.0198685131113052 1102 9 1.2492639830715078 1102 10 1.1919180577467827 1102 11 1.2808898847501098 1102 12 1.3786552468565816 1102 13 1.3421963080946995 1102 14 1.5036263780789128 1102 15 1.694213128445405 1102 16 1.5183752335408869 1102 17 1.643885317598027 1102 18 1.8132146406605187 1102 19 1.544032604496401 1102 20 1.7092313202646565 1102 21 1.5444129081016271 1102 22 1.5471169064115955 1102 23 1.7385754388839132 1102 24 1.835617973897996 1102 25 1.6233869424721377 1103 1 0.4983254894304139 1103 2 0.4335708969177361 1103 3 0.4407663228236842 1103 4 0.5213841735749511 1103 5 0.23902231292064613 1103 6 0.37078920584787056 1103 7 0.4353794008568528 1103 8 0.41915777659479403 1103 9 0.5492370505939519 1103 10 0.606799656048878 1103 11 0.48743348541582066 1103 12 0.5598982220031061 1103 13 0.43674025078635736 1103 14 0.3466926489097588 1103 15 0.6880180389961197 1103 16 0.7265368482337919 1103 17 0.4805022514345936 1103 18 0.8908456483689022 1103 19 0.7026857575583847 1103 20 0.5589551177935661 1103 21 0.7711699114752122 1103 22 1.0275129955845301 1103 23 0.8838154332826207 1103 24 0.688624476934713 1103 25 0.7566271136668661 1104 1 0.39583245689790303 1104 2 0.2617512617874098 1104 3 0.42541942684951906 1104 4 0.3891749223197607 1104 5 0.49939763680450255 1104 6 0.5296988523879023 1104 7 0.5089591495542933 1104 8 0.43073409033678445 1104 9 0.6428704995766736 1104 10 0.7724069839538359 1104 11 0.8057349408958885 1104 12 0.6918461179523803 1104 13 0.8047694711927846 1104 14 1.041896558239138 1104 15 1.0409215719899194 1104 16 1.2671741858301786 1104 17 1.3102407114536518 1104 18 1.0974941901187094 1104 19 1.1254406110990445 1104 20 1.2011139367084596 1104 21 1.0575739756104383 1104 22 1.0513565485392888 1104 23 0.9540035850148314 1104 24 1.237854020763315 1104 25 1.1638383521858984 1105 1 0.3942170025091869 1105 2 0.38928595095299556 1105 3 0.3131921987593529 1105 4 0.30742768283521116 1105 5 0.46891390004486055 1105 6 0.36496748258753087 1105 7 0.49908276367433757 1105 8 0.2819885974920098 1105 9 0.45779789783303215 1105 10 0.5279711186175149 1105 11 0.6238032922328571 1105 12 0.6163170660104598 1105 13 0.5194412453440123 1105 14 0.633164108970405 1105 15 0.6000299611082767 1105 16 0.8170995975648978 1105 17 0.30789436702828255 1105 18 0.7980091864830001 1105 19 0.6602009916116387 1105 20 0.7302970640182593 1105 21 0.6452432984689338 1105 22 0.7224672765057676 1105 23 0.8036763711676471 1105 24 0.8869555882413351 1105 25 0.7500533912791454 1106 1 -0.14123258402398875 1106 2 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-0.5294586778108368 2315 6 -0.7544535753783421 2315 7 -0.7098546296253794 2315 8 -0.6437824450624028 2315 9 -0.6918943252599377 2315 10 -0.7107636157476236 2315 11 -0.6758868319331697 2315 12 -0.7813593174897022 2315 13 -0.8352386775731802 2315 14 -0.8659150303164722 2315 15 -0.9137402752726445 2315 16 -1.0917459763779789 2315 17 -0.766120523060998 2315 18 -0.7805334717140511 2315 19 -1.1492324442250919 2315 20 -0.9970706473419426 2315 21 -0.8738783898939886 2315 22 -0.9762634177148968 2315 23 -1.0239973196759493 2315 24 -0.6062727631825879 2315 25 -0.8613538244374368 2316 1 -1.474991438461899 2316 2 -1.481685735633878 2316 3 -1.345297675500138 2316 4 -1.669462915884587 2316 5 -1.740718606539474 2316 6 -1.852256519152185 2316 7 -1.8596799388063303 2316 8 -1.7716172629044067 2316 9 -1.785094648609217 2316 10 -2.105934800294616 2316 11 -2.021828462156609 2316 12 -2.196248580325584 2316 13 -2.170367039515124 2316 14 -2.5578849822500302 2316 15 -2.4669353423277585 2316 16 -2.446046110774695 2316 17 -2.5195931025188543 2316 18 -2.1967031777726156 2316 19 -2.260651930129626 2316 20 -2.2639486452668294 2316 21 -2.210701719868446 2316 22 -2.207581962842429 2316 23 -2.247498933308435 2316 24 -2.4340738261497528 2316 25 -2.3680334283968456 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64994.0 3 2.0 4 1072.0 5 9594.0 6 11379.0 7 9256.0 8 7578.0 9 6734.0 10 6451.0 11 5913.0 12 5806.0 13 5531.0 14 5404.0 15 5275.0 16 5658.0 17 5705.0 18 6068.0 19 6886.0 20 8295.0 21 10526.0 22 13773.0 23 16708.0 24 20364.0 25 23719.0 26 26686.0 27 30438.0 28 35836.0 29 43333.0 30 53577.0 31 67667.0 32 85440.0 33 107334.0 34 136856.0 35 178363.0 36 251806.0 37 395998.0 38 485626.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.58427611711926 19.95464978966437 14.80926303354179 23.65181105967458 2 16.391591427108263 21.05728445526128 38.36456486269061 24.18655925493986 3 19.738847760346143 24.515520775555352 29.936007246313117 25.809624217785387 4 14.444144776376946 17.60686623326337 36.06720048703514 31.881788503324543 5 14.388087715109144 37.24514066661854 34.541618772084725 13.82515284618759 6 33.147117642949766 36.52439732408238 16.880338222960138 13.44814681000772 7 29.02337148781186 31.63484762341377 20.98363704409269 18.35814384468168 8 26.349165522093994 36.103654105126125 19.41076519752726 18.13641517525262 9 26.5546566027541 15.673852994771126 19.489455050792195 38.28203535168258 10 16.050787106706863 28.98862952437743 32.588193006179075 22.37239036273663 11 35.354319453047694 22.4407177661889 23.11700639927537 19.087956381488038 12 23.800650521291363 24.473978454431357 30.250119006259567 21.47525201801771 13 29.25421356176367 21.316623266197922 25.536268343039648 23.892894828998763 14 22.14261229032809 21.314957872478026 26.610401031433845 29.93202880576004 15 24.03343555458305 29.41904127909639 23.06149327527894 23.486029891041614 16 23.610101723173628 27.72667743312866 25.864720993351842 22.79849985034587 17 22.7643130181514 27.902654036197333 26.626129749899498 22.706903195751764 18 22.695014135029197 27.120982989390978 28.1108745121206 22.073128363459226 19 24.229304360417107 26.5940246598549 26.921552091433814 22.255118888294177 20 24.296799067009427 26.52884300009576 27.199256494225942 21.97510143866887 21 24.151123377455473 26.062532758525776 26.759685074047567 23.026658789971183 22 24.175364108267246 26.31312825243298 27.009447871094828 22.50205976820495 23 23.77108978276327 26.378633738748764 26.99524576353907 22.8550307149489 24 23.98550922419947 26.1465888804437 27.075045879283937 22.7928560160729 25 23.797486274533387 26.355347900733427 27.171728060188027 22.675437764545155 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 293.0 1 293.0 2 458.0 3 623.0 4 623.0 5 623.0 6 1525.0 7 2427.0 8 2427.0 9 2427.0 10 3348.5 11 4270.0 12 4270.0 13 4270.0 14 6532.0 15 8794.0 16 8794.0 17 8794.0 18 15566.5 19 22339.0 20 22339.0 21 22339.0 22 34437.5 23 46536.0 24 46536.0 25 46536.0 26 66532.0 27 86528.0 28 86528.0 29 86528.0 30 110784.5 31 135041.0 32 135041.0 33 135041.0 34 155501.5 35 175962.0 36 175962.0 37 175962.0 38 196357.5 39 216753.0 40 216753.0 41 216753.0 42 236079.0 43 255405.0 44 255405.0 45 255405.0 46 267850.5 47 280296.0 48 280296.0 49 280296.0 50 280696.0 51 281096.0 52 281096.0 53 281096.0 54 259604.0 55 238112.0 56 238112.0 57 238112.0 58 207156.0 59 176200.0 60 176200.0 61 176200.0 62 146095.5 63 115991.0 64 115991.0 65 115991.0 66 91049.5 67 66108.0 68 66108.0 69 66108.0 70 48355.5 71 30603.0 72 30603.0 73 30603.0 74 21435.0 75 12267.0 76 12267.0 77 12267.0 78 8239.5 79 4212.0 80 4212.0 81 4212.0 82 2777.0 83 1342.0 84 1342.0 85 1342.0 86 862.0 87 382.0 88 382.0 89 382.0 90 223.0 91 64.0 92 64.0 93 64.0 94 34.5 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37878454940228556 2 0.0 3 0.0 4 0.0 5 0.030300913514716296 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.007309227992862863 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2161651.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.06038920064694 #Duplication Level Percentage of deduplicated Percentage of total 1 83.08645675823992 58.21069497785011 2 10.893345602176021 15.263840651712158 3 2.9219081326748273 6.141300629512019 4 1.1930830170134419 3.343514420825753 5 0.5779279589842917 2.0244928868187504 6 0.33206177519307734 1.3958626325210837 7 0.21767052100046272 1.0675056993160008 8 0.14007752952632177 0.7851108989503374 9 0.09813547691204305 0.6187868736163955 >10 0.4992922433266091 6.05840755126841 >50 0.024704698943264415 1.195360311553399 >100 0.013156779181011094 1.732283771144311 >500 0.001320913229514451 0.6510448940440207 >1k 7.925479377084801E-4 1.1863176256927999 >5k 6.604566147570667E-5 0.3254761751744736 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7034 0.32539942849238845 No Hit TATCAACGCAGAGTACTTTTTTTTT 4980 0.23037946458517125 No Hit GGTATCAACGCAGAGTACTTTTTTT 4550 0.21048726181978494 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2425 0.11218277140944584 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.6260936663689E-5 0.0 0.0 0.0 6 0.0 4.6260936663689E-5 0.0 0.0 0.0 7 0.0 4.6260936663689E-5 0.0 0.0 0.0 8 0.0 9.2521873327378E-5 0.0 0.0 0.0 9 0.0 9.2521873327378E-5 0.0 0.0 0.0 10 0.0 9.2521873327378E-5 0.0 0.0 0.0 11 0.0 9.2521873327378E-5 0.0 4.6260936663689E-5 0.0 12 0.0 1.38782809991067E-4 0.0 4.6260936663689E-5 0.0 13 0.0 1.38782809991067E-4 0.0 4.6260936663689E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1710 0.0 12.319572 1 AATCCCG 250 0.0 11.777447 19 CCGTGCG 60 0.0058884323 11.08042 9 AAGCGTG 60 0.0058884323 11.08042 7 TAGGACC 405 0.0 11.025362 4 TGTAGGA 1220 0.0 10.980177 2 TAGACCG 70 0.0014910536 10.857803 5 GTAGGAC 1175 0.0 10.430423 3 TAGGACG 1145 0.0 10.371811 4 GGACGTG 1135 0.0 10.292447 6 TTAGGCA 130 3.8396684E-7 10.231393 4 GATATAC 560 0.0 10.213221 1 TTAGGAC 760 0.0 10.125616 3 GACGTGG 565 0.0 10.085843 7 TACACCG 85 6.591471E-4 10.059436 5 TAGGACT 370 0.0 10.014124 4 GTACCGA 95 1.6488673E-4 9.997371 6 AGGACCG 115 1.025991E-5 9.913648 5 AGGACGT 1185 0.0 9.86136 5 AAGTCGT 145 1.6238482E-7 9.825003 7 >>END_MODULE