FastQCFastQC Report
Thu 26 May 2016
SRR1512165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732199
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT30090.410953852709441No Hit
CTGTAGGACGTGGAATATGGCAAGA27550.3762638299150914No Hit
GTATCAACGCAGAGTACTTTTTTTT27300.37284945759281285No Hit
GTCCTAAAGTGTGTATTTCTCATTT27250.37216658312835715No Hit
CTTTAGGACGTGAAATATGGCGAGG21940.2996453150031617No Hit
GGTATCAACGCAGAGTACTTTTTTT17720.2420107102031005No Hit
TATCAACGCAGAGTACTTTTTTTTT17170.2344990910940878No Hit
GTCCTACAGTGTGCATTTCTCATTT12760.17426956332909496No Hit
CTGAAGGACCTGGAATATGGCGAGA10790.14736430942954032No Hit
GTCCTTCAGTGTGCATTTCTCATTT9290.1268780754958693No Hit
ATTTAGAAATGTCCACTGTAGGACG8620.11772755767216289No Hit
TTTCTAAATTTTCCACCTTTTTCAG8580.11718125810059833No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8180.11171826238495272No Hit
GAATATGGCAAGAAAACTGAAAATC8070.11021593856315019No Hit
CTGTAGGACCTGGAATATGGCGAGA8020.10953306409869448No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7790.10639184156219826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG350.00227265716.1719639
GTCTTAC551.16985706E-414.6813011
GCGCCAC457.1138935E-414.67756713
GGTATCA4700.014.3884541
TGCGCCA400.005519118614.15336812
TAGGACC3450.013.9463414
TCCAACG955.3751137E-913.90506418
ATAAAGC707.788696E-613.4775573
GTCATAC450.00770457713.4578591
GTGCGCC500.001579466313.20710211
GTCTAAG550.001945490712.8461381
TATGTCG751.5829793E-512.58077116
TAGAAAT3450.012.0321374
CGCCACC550.00322601612.00891914
CAACACA1052.9675903E-711.6805494
TATGAAC658.494895E-411.6114343
TCTAGGA1154.019148E-711.4099242
GTGTAGA1154.019148E-711.4099241
TAAGGTG752.208817E-411.3211485
GATATAC2000.011.1027331
Produced by FastQC (version 0.11.5)