##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512165_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 732199 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.915099583583153 31.0 30.0 34.0 25.0 34.0 2 27.29943772116597 31.0 28.0 34.0 7.0 34.0 3 28.827008777668365 31.0 28.0 34.0 16.0 34.0 4 33.305487988921044 37.0 33.0 37.0 28.0 37.0 5 33.665887279277904 37.0 35.0 37.0 30.0 37.0 6 33.85956823213361 37.0 35.0 37.0 30.0 37.0 7 33.875501059138294 37.0 35.0 37.0 30.0 37.0 8 33.879729417822205 37.0 35.0 37.0 30.0 37.0 9 35.466477009665404 39.0 37.0 39.0 30.0 39.0 10 35.346863352722416 39.0 37.0 39.0 30.0 39.0 11 35.360362415135775 39.0 37.0 39.0 30.0 39.0 12 35.2712090565543 39.0 37.0 39.0 30.0 39.0 13 35.27434345034615 39.0 37.0 39.0 30.0 39.0 14 36.4424603147505 40.0 37.0 41.0 30.0 41.0 15 36.40124337782488 40.0 37.0 41.0 30.0 41.0 16 36.36285627267997 40.0 37.0 41.0 30.0 41.0 17 36.35684561164383 40.0 37.0 41.0 30.0 41.0 18 36.3218824390637 40.0 37.0 41.0 30.0 41.0 19 36.34647957727339 40.0 37.0 41.0 29.0 41.0 20 36.29360324174166 40.0 37.0 41.0 29.0 41.0 21 36.2245700963809 40.0 37.0 41.0 29.0 41.0 22 36.143238381915296 40.0 37.0 41.0 29.0 41.0 23 36.04596701170037 40.0 37.0 41.0 29.0 41.0 24 35.98302374081363 40.0 37.0 41.0 28.0 41.0 25 35.88037814856344 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4260063486137362 1101 2 3.3852926156131744 1101 3 1.992834997299095 1101 4 0.6655106304308873 1101 5 0.48910898034113615 1101 6 0.3280486310954842 1101 7 0.12369145616022337 1101 8 0.22058145158439402 1101 9 0.07255410363251968 1101 10 0.2263465187869258 1101 11 0.09931882857277685 1101 12 0.07859903218110986 1101 13 0.07409186046626104 1101 14 -0.04056764432721138 1101 15 0.19163747489708527 1101 16 0.450036933604963 1101 17 0.31754060075878954 1101 18 0.20449839486195742 1101 19 0.18756627834457618 1101 20 0.29783426973502714 1101 21 0.20631435352866845 1101 22 0.3219128877905888 1101 23 0.22154674669042151 1101 24 0.1830383282054484 1101 25 0.38420288348864773 1102 1 1.1994591957805092 1102 2 4.232350078574026 1102 3 2.5285354776357103 1102 4 1.6396323368298837 1102 5 1.128766217708531 1102 6 1.0260639156854268 1102 7 1.1438221862972213 1102 8 0.8247346523498891 1102 9 0.8385309993145569 1102 10 0.7602140523407854 1102 11 0.9177794701199957 1102 12 1.0768706441603726 1102 13 0.9801204652087989 1102 14 1.0336516502496735 1102 15 1.022087521156422 1102 16 1.10379534583263 1102 17 1.3353979699335952 1102 18 1.1520399965754535 1102 19 1.0455802321866372 1102 20 0.9188309365284297 1102 21 1.20759440625163 1102 22 1.0593832628080193 1102 23 0.9539856273413463 1102 24 0.8504812644689608 1102 25 1.0334193264629334 1103 1 0.7872187442940763 1103 2 3.775296539474752 1103 3 2.3181014088889107 1103 4 1.2022095927013297 1103 5 0.8198129645340231 1103 6 0.48504807120851723 1103 7 0.498395421741904 1103 8 0.5145562303049687 1103 9 0.5578830488582014 1103 10 0.6493682644245382 1103 11 0.6267230468015228 1103 12 0.657196461043668 1103 13 0.4896923896447518 1103 14 0.44858435180165657 1103 15 0.6817246838838145 1103 16 0.5846920758022947 1103 17 0.5813931504157992 1103 18 0.6570483620597116 1103 19 0.6299483259799459 1103 20 0.7072403707969528 1103 21 0.9098580813212394 1103 22 0.8621061030772097 1103 23 0.7609536387283882 1103 24 1.0019808087373576 1103 25 1.0729165858304057 1104 1 1.5347827974975559 1104 2 4.606598553232338 1104 3 3.0364917745264357 1104 4 1.9020850480530882 1104 5 1.6296976633121076 1104 6 1.4537264171184177 1104 7 1.3934957822822938 1104 8 1.358527817290593 1104 9 1.4182324909012962 1104 10 1.5986775014599672 1104 11 1.5391380750851553 1104 12 1.635099528208933 1104 13 1.626752294217674 1104 14 1.664886763042162 1104 15 1.365878520685122 1104 16 1.8220688755815715 1104 17 1.5224768291436064 1104 18 1.7063424791566248 1104 19 1.7727335433563454 1104 20 1.623513321538688 1104 21 1.8060760703070642 1104 22 1.77671897809455 1104 23 1.756065378191586 1104 24 1.8633559125895118 1104 25 1.8544973831967297 1105 1 0.9568402120110839 1105 2 3.8647406722478053 1105 3 2.364427584545517 1105 4 1.5598282913178707 1105 5 1.122143415731749 1105 6 0.9513023940212477 1105 7 0.5433276709796999 1105 8 0.6827650192298123 1105 9 0.8401703383432775 1105 10 0.8412487993047364 1105 11 0.8599572530057458 1105 12 0.6727296769663127 1105 13 0.7604250917523601 1105 14 0.6448154372984121 1105 15 0.8784386429942685 1105 16 0.7566513393965906 1105 17 0.725595776060608 1105 18 0.8352740437475532 1105 19 1.0296098456410263 1105 20 1.1162858906205528 1105 21 0.8781431298977012 1105 22 1.0140643977988901 1105 23 0.8654277987392689 1105 24 0.8084969459359712 1105 25 0.7513524503695095 1106 1 0.40152121726928414 1106 2 3.248333157773665 1106 3 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-0.5428030911275599 2315 16 -0.5169859266074042 2315 17 -0.5480335011755955 2315 18 -0.6052066404105219 2315 19 -0.5224759217699884 2315 20 -0.5760946223781716 2315 21 -0.5661142442342708 2315 22 -0.4899190015416366 2315 23 -0.4533280305647338 2315 24 -0.5734140871515123 2315 25 -0.6589486662605069 2316 1 -1.7166618688806992 2316 2 -0.4148868896483435 2316 3 -0.7088364164880083 2316 4 -1.4820829916372702 2316 5 -1.499750703326633 2316 6 -1.5353826669290456 2316 7 -1.7219505041105023 2316 8 -1.7692563525900198 2316 9 -1.6625657344464102 2316 10 -1.5441102122024617 2316 11 -1.779883288856631 2316 12 -1.3921498527798306 2316 13 -1.449598254838392 2316 14 -1.8538382118752281 2316 15 -2.144350582507961 2316 16 -1.8833977530392971 2316 17 -1.8409936838303196 2316 18 -2.345945331154404 2316 19 -2.0605301416244686 2316 20 -2.266577896325657 2316 21 -2.187686873701182 2316 22 -2.3488689675245027 2316 23 -2.1281588097301025 2316 24 -1.9034264255701174 2316 25 -1.8502218296234787 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27985.0 3 0.0 4 220.0 5 1185.0 6 1261.0 7 1167.0 8 1016.0 9 955.0 10 965.0 11 904.0 12 866.0 13 812.0 14 852.0 15 824.0 16 835.0 17 934.0 18 1027.0 19 1333.0 20 1715.0 21 2059.0 22 2389.0 23 2885.0 24 3324.0 25 3781.0 26 4315.0 27 4850.0 28 5782.0 29 6938.0 30 8893.0 31 12005.0 32 16549.0 33 23443.0 34 34301.0 35 53121.0 36 85046.0 37 116442.0 38 301220.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.08664174630121 20.156678717124716 13.833670900943595 26.92300863563048 2 13.779186411651331 20.29251071453099 43.887128096546945 22.04117477727073 3 17.14219768123147 24.297219744905416 30.99239414421489 27.56818842964822 4 11.0480893855359 17.073363935214335 39.80133816080055 32.07720851844922 5 11.559152634734545 37.88382666460894 37.5668363382086 12.990184362447913 6 29.06218118298441 38.55236076531107 19.027340927807877 13.358117123896646 7 25.600428331038707 31.8900380253065 23.722382482134662 18.78715116152014 8 22.23657776096389 39.59579294699938 21.71185702247613 16.455772269560597 9 24.573920477902867 15.45604405359745 22.297353588300446 37.672681880199235 10 14.287177652093034 28.888491286823907 35.95955567513969 20.86477538594337 11 31.55602356816822 22.179700453717285 27.545364899600365 18.718911078514125 12 22.74299746380424 24.202300194346073 33.77852195919415 19.276180382655536 13 27.53650305449748 23.048105774523044 27.022844882333903 22.392546288645573 14 18.32452652899007 23.813334899392107 29.03991947544315 28.822219096174674 15 20.50808591653362 33.97682870367209 24.360317345421123 21.15476803437317 16 19.05424618170743 28.404163349034896 31.748609326153137 20.792981143104537 17 19.058343428494165 29.790535086772856 30.531453880707293 20.619667604025683 18 18.757264950260275 27.177709391062283 34.319385994746696 19.745639663930742 19 21.587971050320487 26.61360044907787 29.73501946960188 22.063409030999757 20 21.51874012392806 27.954695376530154 30.859097048753135 19.66746745078865 21 19.846970622019892 27.194402217041112 29.500633069588485 23.457994091350507 22 20.82236159216809 29.99560219835451 29.530828316379488 19.65120789309791 23 18.89110452490542 29.330893098597322 30.977505224196566 20.800497152300697 24 21.266078328796276 26.71136796067306 31.9949352495974 20.027618460933265 25 19.977600065560782 28.131449371367694 31.476278606013835 20.41467195705769 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 473.0 1 473.0 2 407.5 3 342.0 4 342.0 5 342.0 6 850.0 7 1358.0 8 1358.0 9 1358.0 10 1829.0 11 2300.0 12 2300.0 13 2300.0 14 3526.0 15 4752.0 16 4752.0 17 4752.0 18 8084.0 19 11416.0 20 11416.0 21 11416.0 22 18170.5 23 24925.0 24 24925.0 25 24925.0 26 36000.0 27 47075.0 28 47075.0 29 47075.0 30 61133.0 31 75191.0 32 75191.0 33 75191.0 34 81300.0 35 87409.0 36 87409.0 37 87409.0 38 89781.5 39 92154.0 40 92154.0 41 92154.0 42 93016.0 43 93878.0 44 93878.0 45 93878.0 46 92452.5 47 91027.0 48 91027.0 49 91027.0 50 83142.0 51 75257.0 52 75257.0 53 75257.0 54 63713.0 55 52169.0 56 52169.0 57 52169.0 58 43360.0 59 34551.0 60 34551.0 61 34551.0 62 27453.5 63 20356.0 64 20356.0 65 20356.0 66 15317.5 67 10279.0 68 10279.0 69 10279.0 70 7441.5 71 4604.0 72 4604.0 73 4604.0 74 3203.0 75 1802.0 76 1802.0 77 1802.0 78 1214.0 79 626.0 80 626.0 81 626.0 82 399.0 83 172.0 84 172.0 85 172.0 86 116.0 87 60.0 88 60.0 89 60.0 90 37.0 91 14.0 92 14.0 93 14.0 94 11.0 95 8.0 96 8.0 97 8.0 98 4.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 6.534835475055278 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0075116191090127135 8 0.0 9 0.0 10 0.004370396572516488 11 0.003414372322278506 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.012018590574420342 19 0.004370396572516488 20 0.0 21 0.007101894430339293 22 0.002048623393367104 23 0.003960671893843067 24 0.01092599143129122 25 0.007375044216121574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 732199.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.56114821334789 #Duplication Level Percentage of deduplicated Percentage of total 1 90.54012018369133 69.31855560637922 2 6.798397422586404 10.409862253677598 3 1.3123615856531146 3.0142772960607704 4 0.47000839361989366 1.4393752914180094 5 0.22112369901247747 0.8464742146789008 6 0.14186520883734757 0.651681796806823 7 0.08412747706596574 0.45086273683237 8 0.06595747824671747 0.40398242142604845 9 0.05015955120987613 0.3456245551044905 >10 0.26319297181017764 3.734206369421781 >50 0.024163209826103208 1.3079032035585743 >100 0.023617620368519644 3.690798326234464 >500 0.003270132048030098 1.7079070143593413 >1k 0.001635066024015049 2.678488914041684 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3009 0.410953852709441 No Hit CTGTAGGACGTGGAATATGGCAAGA 2755 0.3762638299150914 No Hit GTATCAACGCAGAGTACTTTTTTTT 2730 0.37284945759281285 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2725 0.37216658312835715 No Hit CTTTAGGACGTGAAATATGGCGAGG 2194 0.2996453150031617 No Hit GGTATCAACGCAGAGTACTTTTTTT 1772 0.2420107102031005 No Hit TATCAACGCAGAGTACTTTTTTTTT 1717 0.2344990910940878 No Hit GTCCTACAGTGTGCATTTCTCATTT 1276 0.17426956332909496 No Hit CTGAAGGACCTGGAATATGGCGAGA 1079 0.14736430942954032 No Hit GTCCTTCAGTGTGCATTTCTCATTT 929 0.1268780754958693 No Hit ATTTAGAAATGTCCACTGTAGGACG 862 0.11772755767216289 No Hit TTTCTAAATTTTCCACCTTTTTCAG 858 0.11718125810059833 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 818 0.11171826238495272 No Hit GAATATGGCAAGAAAACTGAAAATC 807 0.11021593856315019 No Hit CTGTAGGACCTGGAATATGGCGAGA 802 0.10953306409869448 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 779 0.10639184156219826 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3657489289114024E-4 0.0 10 0.0 0.0 0.0 1.3657489289114024E-4 0.0 11 0.0 0.0 0.0 2.731497857822805E-4 0.0 12 0.0 0.0 0.0 2.731497857822805E-4 0.0 13 0.0 0.0 0.0 2.731497857822805E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTGCG 35 0.002272657 16.171963 9 GTCTTAC 55 1.16985706E-4 14.681301 1 GCGCCAC 45 7.1138935E-4 14.677567 13 GGTATCA 470 0.0 14.388454 1 TGCGCCA 40 0.0055191186 14.153368 12 TAGGACC 345 0.0 13.946341 4 TCCAACG 95 5.3751137E-9 13.905064 18 ATAAAGC 70 7.788696E-6 13.477557 3 GTCATAC 45 0.007704577 13.457859 1 GTGCGCC 50 0.0015794663 13.207102 11 GTCTAAG 55 0.0019454907 12.846138 1 TATGTCG 75 1.5829793E-5 12.580771 16 TAGAAAT 345 0.0 12.032137 4 CGCCACC 55 0.003226016 12.008919 14 CAACACA 105 2.9675903E-7 11.680549 4 TATGAAC 65 8.494895E-4 11.611434 3 TCTAGGA 115 4.019148E-7 11.409924 2 GTGTAGA 115 4.019148E-7 11.409924 1 TAAGGTG 75 2.208817E-4 11.321148 5 GATATAC 200 0.0 11.102733 1 >>END_MODULE