FastQCFastQC Report
Thu 26 May 2016
SRR1512165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732199
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47140.6438140450888351No Hit
TATCAACGCAGAGTACTTTTTTTTT36190.4942645373730366No Hit
GGTATCAACGCAGAGTACTTTTTTT35340.4826556714772896No Hit
CTGTAGGACGTGGAATATGGCAAGA25110.34293955604965315No Hit
CTTTAGGACGTGAAATATGGCGAGG19370.26454556753013864No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18380.2510246531339158No Hit
GTCCTAAAGTGTGTATTTCTCATTT16650.2273971966637485No Hit
GTCCTACAGTGGACATTTCTAAATT16430.22439254902014344No Hit
CTGAAGGACCTGGAATATGGCGAGA13320.18191775733099882No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11420.15596852768168218No Hit
TTTCTAAATTTTCCACCTTTTTCAG10960.1496860826086897No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10730.1465448600721935No Hit
ATTTAGAAATGTCCACTGTAGGACG9880.13493599417644658No Hit
GAATATGGCAAGAAAACTGAAAATC9680.13220449631862377No Hit
GGAATATGGCGAGAAAACTGAAAAT9650.13179477163995035No Hit
CTGTAGGACCTGGAATATGGCGAGA8370.11431318534988438No Hit
ACAGTGGACATTTCTAAATTTTCCA7550.10311404413281089No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAC508.705778E-515.1996173
CGTTGGA500.001501599213.29603213
GTGGTAT3200.013.11862851
TAGGACC3200.012.7653054
TGGTATC3100.012.5641992
GGTATCA8650.012.3534331
ACTGATC550.003072640212.0873018
TACTTGT658.034617E-411.688827
AAGTCGT1800.011.6076477
GAACAGT2100.011.306156
TATAGTG1104.955473E-711.226995
AACAGTG2800.011.1930897
ACTGTTC1855.456968E-1210.7805678
AGAACAG3000.010.7663965
TGTAGGA14950.010.7388612
GACATGG3100.010.7226077
CTGTAGG14450.010.6962831
AGGACCT8200.010.6582695
AAATGTC3750.010.6368257
TCAGGAC909.500814E-510.5552893
Produced by FastQC (version 0.11.5)