##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512165_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 732199 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.808808807441693 31.0 28.0 31.0 16.0 34.0 2 28.019125948000475 31.0 28.0 31.0 16.0 34.0 3 27.970259451324026 31.0 27.0 31.0 16.0 34.0 4 31.42889979363534 35.0 32.0 37.0 20.0 37.0 5 30.78372955986009 35.0 30.0 37.0 16.0 37.0 6 30.75173825694927 35.0 31.0 37.0 11.0 37.0 7 30.439035016436787 35.0 30.0 37.0 10.0 37.0 8 30.247726369470595 35.0 30.0 37.0 10.0 37.0 9 31.263160698116224 35.0 30.0 39.0 10.0 39.0 10 30.885603503965452 35.0 30.0 39.0 8.0 39.0 11 30.92936483114563 35.0 30.0 39.0 2.0 39.0 12 30.62653049239346 35.0 30.0 39.0 2.0 39.0 13 30.599873804798968 35.0 30.0 39.0 2.0 39.0 14 31.000400164436172 36.0 29.0 39.0 2.0 41.0 15 30.914444024097275 36.0 29.0 39.0 2.0 41.0 16 30.72089008589195 36.0 29.0 39.0 2.0 41.0 17 30.617012588107876 36.0 28.0 39.0 2.0 40.0 18 30.467083402189843 36.0 28.0 39.0 2.0 40.0 19 30.508391844293698 36.0 28.0 39.0 2.0 40.0 20 30.31574203187931 36.0 27.0 39.0 2.0 40.0 21 30.08709244344775 36.0 27.0 39.0 2.0 40.0 22 30.1468521535812 36.0 27.0 39.0 2.0 40.0 23 29.89409026780971 36.0 27.0 39.0 2.0 40.0 24 29.637841625022705 36.0 27.0 39.0 2.0 41.0 25 29.05673184475805 36.0 27.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.22229617748267572 1101 2 -0.1656685163075089 1101 3 0.057464213936892605 1101 4 -0.0675557079106035 1101 5 -0.10242339174271464 1101 6 -0.277243993920095 1101 7 -0.37033018544303076 1101 8 -0.8790248174484603 1101 9 -0.6267083765655457 1101 10 -0.4698402380042772 1101 11 -0.642905587728869 1101 12 -0.4669410901786968 1101 13 -0.850804851071338 1101 14 -1.0418184948178535 1101 15 -0.8681225737889235 1101 16 -0.9333789930719298 1101 17 -0.8942120465063716 1101 18 -0.615967518166002 1101 19 -0.5605884881391319 1101 20 -0.4572038123344129 1101 21 -0.5171508675622505 1101 22 -0.5925229112777295 1101 23 -0.9387177537830098 1101 24 -0.9259075371407484 1101 25 -0.72076308411566 1102 1 -0.05104001485261378 1102 2 0.21816511485987533 1102 3 -0.08404498089727142 1102 4 0.1251286640397673 1102 5 0.07068948102511641 1102 6 -0.022144377935909176 1102 7 -0.19726325516154475 1102 8 -0.010914606176392283 1102 9 0.38702842685130534 1102 10 0.0855426371715744 1102 11 0.4177923578498799 1102 12 0.45800631359555766 1102 13 0.2937666481112018 1102 14 0.31857971564309295 1102 15 0.529557172228337 1102 16 0.4361300876981282 1102 17 0.3561903463050555 1102 18 0.41802358483388247 1102 19 0.27696363281951264 1102 20 0.3899740516251313 1102 21 0.40532039462042846 1102 22 0.16829258369711297 1102 23 0.22089911756535585 1102 24 0.15533462095210027 1102 25 0.24572107998456616 1103 1 0.09003544930690666 1103 2 0.13644401011652008 1103 3 0.10589521213315578 1103 4 0.09331641297535143 1103 5 0.0861390945896865 1103 6 0.5844453137897503 1103 7 -0.08623330173357147 1103 8 0.409351091592395 1103 9 0.13854389051139293 1103 10 0.3639400226455969 1103 11 0.34402314532912115 1103 12 0.494319694624636 1103 13 0.5566098514673854 1103 14 0.4357526813945505 1103 15 0.30380615151439727 1103 16 0.3845902767331033 1103 17 -0.261288521482836 1103 18 0.4675797147361216 1103 19 0.7998329314922721 1103 20 -0.00666695672786588 1103 21 0.3119796946666291 1103 22 0.6211259232733433 1103 23 0.5178956589994748 1103 24 0.08024191390636304 1103 25 0.31571021734082194 1104 1 0.5331992886489871 1104 2 0.4083503849631782 1104 3 0.5592798181652334 1104 4 0.42965087225049814 1104 5 0.7178594786049501 1104 6 0.5167422818523875 1104 7 0.5477722193821251 1104 8 0.7240453337015786 1104 9 0.8566505624570517 1104 10 0.9015224224136524 1104 11 0.7654906522694844 1104 12 0.872840341134502 1104 13 0.9433950251985976 1104 14 1.2262121560624202 1104 15 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8 -0.408851319278984 2315 9 -0.5722862842406542 2315 10 -0.2611290560641031 2315 11 -0.46725479615462007 2315 12 -0.36691948467147384 2315 13 -0.015385297889086047 2315 14 -0.14690451050464048 2315 15 0.1394056570768214 2315 16 0.1452293744040496 2315 17 0.15768874259018162 2315 18 -0.16793074519627638 2315 19 -0.08445266283848696 2315 20 0.008906264751658455 2315 21 0.01869346178496656 2315 22 -0.3015898903322807 2315 23 -0.40970790048242023 2315 24 -0.11915195092783648 2315 25 -0.40995894299403446 2316 1 -1.826644600858657 2316 2 -1.7517770070895473 2316 3 -1.6501070615578897 2316 4 -1.8106580350681014 2316 5 -2.1848163067799433 2316 6 -2.0076509030409753 2316 7 -1.9198983653878976 2316 8 -1.7672443267396893 2316 9 -2.09166913268567 2316 10 -1.9432175928660307 2316 11 -2.32315359805958 2316 12 -1.9902886801373931 2316 13 -2.1480106135565684 2316 14 -2.377070214313406 2316 15 -2.3861417881786515 2316 16 -2.406683080480004 2316 17 -2.574009461996944 2316 18 -2.6366545837128967 2316 19 -2.2390634260907554 2316 20 -2.2443380474679913 2316 21 -2.5066717899994906 2316 22 -2.4750403809862185 2316 23 -2.346117766604813 2316 24 -2.1552493206537378 2316 25 -2.289226940364916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45182.0 3 3.0 4 739.0 5 5140.0 6 6110.0 7 5152.0 8 4042.0 9 3576.0 10 3395.0 11 3035.0 12 2966.0 13 2834.0 14 2827.0 15 2793.0 16 2743.0 17 3009.0 18 3179.0 19 3585.0 20 4466.0 21 5482.0 22 6980.0 23 8445.0 24 9865.0 25 11513.0 26 12995.0 27 14799.0 28 16946.0 29 20165.0 30 24654.0 31 30228.0 32 36808.0 33 45341.0 34 55758.0 35 68760.0 36 85051.0 37 99899.0 38 73734.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.146588822191156 24.72891483704739 16.239892552695775 19.884603788065675 2 16.88994385406153 25.466027678267793 39.367030001406725 18.27699846626395 3 20.904426255703708 28.512467239097568 31.246833169671085 19.336273335527636 4 16.149161635019986 21.5550690454371 37.42070120281508 24.87506811672783 5 12.586816465483794 40.78393014245824 35.58245583357468 11.046797558483288 6 29.97614036621192 40.87386079467467 17.430234130338885 11.719764708774527 7 26.661604290636838 34.32454838097293 23.022429694659515 15.99141763373072 8 24.362912268386054 40.84545321695332 20.3280802077031 14.463554306957535 9 26.424783426363597 16.315373279668506 21.90114982402325 35.35869346994465 10 15.820289292938122 32.0358263259032 33.751070405723034 18.39281397543564 11 33.14768252892998 23.477224087987008 26.30896791719191 17.0661254658911 12 23.859087488510635 26.87466112354701 32.3257748235111 16.940476564431254 13 29.905394571694305 23.90757157548699 26.233851726101783 19.953182126716918 14 20.41152746725958 25.733168168762866 28.344343545948576 25.510960818028977 15 22.15517912480077 35.598928706540164 23.528712822606966 18.7171793460521 16 20.408249669830198 30.827001948923723 29.87370919654356 18.891039184702517 17 20.54031759125593 31.91331864697985 28.760077519909206 18.786286241855016 18 20.314286143521095 28.811293104743385 33.096466944095795 17.777953807639726 19 23.378207290640933 27.86004897575659 29.910584417624168 18.851159315978304 20 23.312514767160295 29.31907855651264 30.131835744107814 17.236570932219248 21 21.845017543044992 28.2791973220395 29.43079681889759 20.444988316017913 22 22.7020249959369 30.171032738367575 29.72962268454341 17.397319581152118 23 20.750506351415392 30.458386313010532 30.423559715323297 18.36754762025078 24 22.65791130553306 28.6876928266769 30.86387717000433 17.79051869778571 25 21.688386012022242 29.722472249273707 30.690998889561193 17.898142849142857 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 550.0 1 550.0 2 566.0 3 582.0 4 582.0 5 582.0 6 1201.5 7 1821.0 8 1821.0 9 1821.0 10 2563.5 11 3306.0 12 3306.0 13 3306.0 14 4640.5 15 5975.0 16 5975.0 17 5975.0 18 9993.5 19 14012.0 20 14012.0 21 14012.0 22 21996.0 23 29980.0 24 29980.0 25 29980.0 26 42463.0 27 54946.0 28 54946.0 29 54946.0 30 67940.5 31 80935.0 32 80935.0 33 80935.0 34 86701.5 35 92468.0 36 92468.0 37 92468.0 38 92097.0 39 91726.0 40 91726.0 41 91726.0 42 91439.0 43 91152.0 44 91152.0 45 91152.0 46 87977.5 47 84803.0 48 84803.0 49 84803.0 50 77089.0 51 69375.0 52 69375.0 53 69375.0 54 58089.5 55 46804.0 56 46804.0 57 46804.0 58 38414.0 59 30024.0 60 30024.0 61 30024.0 62 23832.0 63 17640.0 64 17640.0 65 17640.0 66 13390.0 67 9140.0 68 9140.0 69 9140.0 70 6690.0 71 4240.0 72 4240.0 73 4240.0 74 2996.0 75 1752.0 76 1752.0 77 1752.0 78 1202.0 79 652.0 80 652.0 81 652.0 82 443.0 83 234.0 84 234.0 85 234.0 86 149.5 87 65.0 88 65.0 89 65.0 90 36.5 91 8.0 92 8.0 93 8.0 94 7.5 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3467636530506051 2 0.0 3 0.0 4 0.0 5 0.026632104113772346 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0076481940019038535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 732199.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.76828843964603 #Duplication Level Percentage of deduplicated Percentage of total 1 91.81895078704314 70.48783698245391 2 5.754714985676582 8.835592398167465 3 1.1493958412934544 2.6471145442723656 4 0.4172209357269913 1.2811734854779473 5 0.221839445834057 0.8515117282540058 6 0.13175731743890182 0.6068870249510172 7 0.08831606288892783 0.47459110927977916 8 0.069579987539979 0.4273229242476868 9 0.04078450178189951 0.2817860756994107 >10 0.2519927158649027 3.506796271825299 >50 0.024169770279414925 1.2827241853482452 >100 0.025413089519391335 3.86186538719789 >500 0.003731992162364423 1.893483225764144 >1k 0.002132566949922527 3.5613146570608265 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4714 0.6438140450888351 No Hit TATCAACGCAGAGTACTTTTTTTTT 3619 0.4942645373730366 No Hit GGTATCAACGCAGAGTACTTTTTTT 3534 0.4826556714772896 No Hit CTGTAGGACGTGGAATATGGCAAGA 2511 0.34293955604965315 No Hit CTTTAGGACGTGAAATATGGCGAGG 1937 0.26454556753013864 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1838 0.2510246531339158 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1665 0.2273971966637485 No Hit GTCCTACAGTGGACATTTCTAAATT 1643 0.22439254902014344 No Hit CTGAAGGACCTGGAATATGGCGAGA 1332 0.18191775733099882 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1142 0.15596852768168218 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1096 0.1496860826086897 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1073 0.1465448600721935 No Hit ATTTAGAAATGTCCACTGTAGGACG 988 0.13493599417644658 No Hit GAATATGGCAAGAAAACTGAAAATC 968 0.13220449631862377 No Hit GGAATATGGCGAGAAAACTGAAAAT 965 0.13179477163995035 No Hit CTGTAGGACCTGGAATATGGCGAGA 837 0.11431318534988438 No Hit ACAGTGGACATTTCTAAATTTTCCA 755 0.10311404413281089 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3657489289114024E-4 2 0.0 0.0 0.0 0.0 1.3657489289114024E-4 3 0.0 0.0 0.0 0.0 1.3657489289114024E-4 4 0.0 0.0 0.0 0.0 1.3657489289114024E-4 5 0.0 0.0 0.0 0.0 1.3657489289114024E-4 6 0.0 0.0 0.0 0.0 1.3657489289114024E-4 7 0.0 0.0 0.0 0.0 1.3657489289114024E-4 8 0.0 0.0 0.0 0.0 1.3657489289114024E-4 9 0.0 0.0 0.0 0.0 1.3657489289114024E-4 10 0.0 0.0 0.0 0.0 1.3657489289114024E-4 11 0.0 0.0 0.0 0.0 1.3657489289114024E-4 12 0.0 0.0 0.0 0.0 1.3657489289114024E-4 13 0.0 0.0 0.0 0.0 1.3657489289114024E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAGAC 50 8.705778E-5 15.199617 3 CGTTGGA 50 0.0015015992 13.296032 13 GTGGTAT 320 0.0 13.1186285 1 TAGGACC 320 0.0 12.765305 4 TGGTATC 310 0.0 12.564199 2 GGTATCA 865 0.0 12.353433 1 ACTGATC 55 0.0030726402 12.087301 8 TACTTGT 65 8.034617E-4 11.68882 7 AAGTCGT 180 0.0 11.607647 7 GAACAGT 210 0.0 11.30615 6 TATAGTG 110 4.955473E-7 11.22699 5 AACAGTG 280 0.0 11.193089 7 ACTGTTC 185 5.456968E-12 10.780567 8 AGAACAG 300 0.0 10.766396 5 TGTAGGA 1495 0.0 10.738861 2 GACATGG 310 0.0 10.722607 7 CTGTAGG 1445 0.0 10.696283 1 AGGACCT 820 0.0 10.658269 5 AAATGTC 375 0.0 10.636825 7 TCAGGAC 90 9.500814E-5 10.555289 3 >>END_MODULE