FastQCFastQC Report
Thu 26 May 2016
SRR1512164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088819
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT40040.36773788848284245No Hit
GTATCAACGCAGAGTACTTTTTTTT39460.3624110159723517No Hit
CTGTAGGACGTGGAATATGGCAAGA32310.29674353588612984No Hit
GTCCTAAAGTGTGTATTTCTCATTT30230.2776402689519562No Hit
CTTTAGGACGTGAAATATGGCGAGG24430.22437154384704894No Hit
GGTATCAACGCAGAGTACTTTTTTT22920.21050330679387486No Hit
TATCAACGCAGAGTACTTTTTTTTT22390.2056356474308402No Hit
GTCCTACAGTGTGCATTTCTCATTT18960.17413362551535197No Hit
CTGTAGGACCTGGAATATGGCGAGA12840.11792593626672569No Hit
CTGAAGGACCTGGAATATGGCGAGA12480.11461960160504178No Hit
GATATACACTGTTCTACAATGCCGG11440.10506796813795496No Hit
GATATACACTGTTCTACAAATCCCG11330.10405769921355155No Hit
GTCCTTCAGTGTGCATTTCTCATTT10890.10001662351593792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2450.016.1767518
CCAACGA1550.015.82886419
TACAACG509.223285E-515.098318
ACCACGG400.00551975414.1540078
CCAACGT1102.0736479E-1013.72572819
GCCGGTT2100.012.58133911
ACGAAAT604.3302038E-412.58133912
TAGGACC5950.012.3704564
GATATAC7250.012.2343321
GGTATCA6500.011.9402381
CCGGTTT2300.011.89757112
GTCCTAT1703.6379788E-1211.8581431
TGTAGGA17850.011.6322732
ATCCCGT1657.2759576E-1211.43758210
CTGTAGG17700.011.3322311
CTAATGG752.2060332E-411.3237263
GTCCTAC20400.011.2652351
ACTGTTC7100.011.1637248
GGTCTAC1001.2447181E-511.0873631
GTAGGAC20100.011.0795993
Produced by FastQC (version 0.11.5)