##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512164_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1088819 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.979176520615457 31.0 30.0 34.0 25.0 34.0 2 27.367844425933054 31.0 28.0 34.0 10.0 34.0 3 28.89479886004928 31.0 28.0 34.0 16.0 34.0 4 33.36676435661023 35.0 33.0 37.0 28.0 37.0 5 33.72852420833949 37.0 35.0 37.0 30.0 37.0 6 33.923025773797114 37.0 35.0 37.0 30.0 37.0 7 33.942613969815 37.0 35.0 37.0 30.0 37.0 8 33.94401273306215 37.0 35.0 37.0 30.0 37.0 9 35.53926410174694 39.0 37.0 39.0 30.0 39.0 10 35.41394116010099 39.0 37.0 39.0 30.0 39.0 11 35.43375896269261 39.0 37.0 39.0 30.0 39.0 12 35.34003998828088 39.0 37.0 39.0 30.0 39.0 13 35.341032807105684 39.0 37.0 39.0 30.0 39.0 14 36.49625144307731 40.0 37.0 41.0 30.0 41.0 15 36.44742330910831 40.0 37.0 41.0 30.0 41.0 16 36.40786668858644 40.0 37.0 41.0 30.0 41.0 17 36.40554858061808 40.0 37.0 41.0 30.0 41.0 18 36.37355887434 40.0 37.0 41.0 30.0 41.0 19 36.40190610193246 40.0 37.0 41.0 30.0 41.0 20 36.335981462483666 40.0 37.0 41.0 29.0 41.0 21 36.268760923532746 40.0 37.0 41.0 29.0 41.0 22 36.18767398438124 40.0 37.0 41.0 29.0 41.0 23 36.08796411524781 40.0 37.0 41.0 29.0 41.0 24 36.0142236680293 40.0 37.0 41.0 29.0 41.0 25 35.90444141771957 40.0 37.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.17424133126442953 1101 2 3.32943957607468 1101 3 1.6241162925180568 1101 4 0.5795060850500349 1101 5 0.19882368290404884 1101 6 0.11747666547412194 1101 7 0.12295242854348487 1101 8 0.17408823407450313 1101 9 0.0018497351317847688 1101 10 0.01901606275623635 1101 11 0.09193433985902288 1101 12 0.024696664503800037 1101 13 -0.08264227486883158 1101 14 -0.05120080518492642 1101 15 0.01383380157009384 1101 16 0.018539721455113067 1101 17 -0.21234576512943448 1101 18 -0.17971697350478877 1101 19 -0.32950597426841455 1101 20 -0.0056333311358045535 1101 21 -0.04936405380400544 1101 22 -0.057442639327121015 1101 23 0.027685895310547437 1101 24 0.18598175756681457 1101 25 0.06458790856899554 1102 1 1.461768374568205 1102 2 4.503564975848931 1102 3 2.7515226308800855 1102 4 1.881436836608728 1102 5 1.491637315907731 1102 6 1.3473151597192015 1102 7 1.3714356703178936 1102 8 1.0933049233857446 1102 9 1.2184714169801296 1102 10 1.3001683594166238 1102 11 1.2348142834831606 1102 12 1.2791062700418365 1102 13 1.2502397449776907 1102 14 1.4817282847195585 1102 15 1.4170429515565388 1102 16 1.5089245124160442 1102 17 1.4711358231062945 1102 18 1.5423289238594862 1102 19 1.5464515734086177 1102 20 1.3785651963017784 1102 21 1.344337079732263 1102 22 1.5590385086412581 1102 23 1.5310284780906187 1102 24 1.6405547557198545 1102 25 1.6695754410902808 1103 1 0.8109476562128108 1103 2 3.690451289144427 1103 3 2.2104391372046237 1103 4 1.1155014569224093 1103 5 0.7821378348249297 1103 6 0.652544499372425 1103 7 0.38898302363627835 1103 8 0.5572488436267804 1103 9 0.5734637946403822 1103 10 0.711042036551575 1103 11 0.5864077025120267 1103 12 0.6070286370382973 1103 13 0.6308762154435925 1103 14 0.7543993931634034 1103 15 0.8957625887834553 1103 16 0.8041411628955757 1103 17 0.809059325127933 1103 18 0.721547360474446 1103 19 0.8761342489144397 1103 20 0.9037800864727998 1103 21 1.0376933763693899 1103 22 0.9595494738680301 1103 23 1.0172678270347362 1103 24 0.9721255150245085 1103 25 0.9390868297426138 1104 1 1.1742413312644295 1104 2 4.098606929743269 1104 3 2.4897387162608418 1104 4 1.5415835788670194 1104 5 1.1287494866963002 1104 6 1.0111287677000078 1104 7 0.8484264598707725 1104 8 0.8031071953275983 1104 9 0.89962384889931 1104 10 0.894531314198936 1104 11 0.8512088658276937 1104 12 0.8820750651633205 1104 13 0.8885036443397425 1104 14 1.120274874946979 1104 15 0.7937183852469261 1104 16 0.9525875367889967 1104 17 1.0160136742769978 1104 18 1.159936777525708 1104 19 1.0455970760201296 1104 20 1.1658423489960938 1104 21 1.2985337203097629 1104 22 1.2541814332202819 1104 23 1.2659381624168944 1104 24 1.3986775531150428 1104 25 1.3465334980166475 1105 1 0.9388638502623472 1105 2 3.9087428978746033 1105 3 2.398696056353863 1105 4 1.5431337019106053 1105 5 0.9198823991253136 1105 6 0.8528976623087701 1105 7 0.6650678736487734 1105 8 0.7566247479697594 1105 9 0.8853883466957626 1105 10 0.8991426218736933 1105 11 0.9079905170856435 1105 12 0.7080919136713462 1105 13 0.8625181820542664 1105 14 0.79329827961201 1105 15 0.8651611029922748 1105 16 0.6965958863465431 1105 17 0.8619628299670481 1105 18 0.860496452808512 1105 19 0.8303828444925969 1105 20 0.8769684907727324 1105 21 0.9147233917755031 1105 22 0.9997885613192423 1105 23 0.627610239437864 1105 24 0.7748921416779808 1105 25 0.8220279166828206 1106 1 -0.04369254646961096 1106 2 2.936948173262433 1106 3 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1107 15 0.26723376480620686 1107 16 0.574903355286807 1107 17 0.47856673124987736 1107 18 0.550851084247931 1107 19 0.5488758855386351 1107 20 0.8406783040773149 1107 21 0.6788554321700104 1107 22 0.6422723012936302 1107 23 0.8106724319296106 1107 24 0.7115152516957295 1107 25 0.9494535811253115 1108 1 0.9716973601928132 1108 2 3.6280911652605035 1108 3 2.225432505645511 1108 4 1.3448033475390204 1108 5 0.8644729970225526 1108 6 0.7592062827462343 1108 7 0.7407991463254504 1108 8 0.6451602047498426 1108 9 0.7318972532562569 1108 10 0.7925807004968775 1108 11 0.8135092667065891 1108 12 0.8890722239828364 1108 13 0.7111212808880154 1108 14 0.8680056456746783 1108 15 0.9322066507823124 1108 16 0.7393702094800503 1108 17 0.9178581417591047 1108 18 0.9521357380004005 1108 19 0.8307189141896032 1108 20 0.6976250039961158 1108 21 0.6739205348263155 1108 22 0.9280301890096752 1108 23 1.0022808590262358 1108 24 1.0580883000659895 1108 25 1.008074245781387 1109 1 0.6653271209899572 1109 2 3.5243898236836237 1109 3 1.7932196541193548 1109 4 0.7813275529473458 1109 5 0.47871186973782187 1109 6 0.44790417283897455 1109 7 0.3328629837755557 1109 8 0.1491414166709717 1109 9 0.4098820367131424 1109 10 0.33559519673025306 1109 11 0.38698461110467974 1109 12 0.37745797985786567 1109 13 0.5135058062339084 1109 14 0.5240907622748168 1109 15 0.49776083908328417 1109 16 0.4989075605410065 1109 17 0.5006029720361198 1109 18 0.43697847037531545 1109 19 0.45754745046019707 1109 20 0.5023325282315341 1109 21 0.4859512080157913 1109 22 0.2247767900481179 1109 23 0.3405285555357338 1109 24 0.4281744371049143 1109 25 0.3954290583507927 1110 1 0.8379477102772128 1110 2 3.7976273885878555 1110 3 2.166685694252063 1110 4 1.2939044821909462 1110 5 0.8880879874472711 1110 6 0.6339850672531995 1110 7 0.5526724952517341 1110 8 0.47942533028821543 1110 9 0.6726485911463627 1110 10 0.5313253924604524 1110 11 0.48281008193014685 1110 12 0.41330639070627484 1110 13 0.47998685604829205 1110 14 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-0.8948687897026986 2315 16 -0.759868815092041 2315 17 -0.6958608518706981 2315 18 -0.7375875987347271 2315 19 -1.139375170892393 2315 20 -0.9395271864294799 2315 21 -0.833861172871508 2315 22 -0.711302874044577 2315 23 -0.843429858471545 2315 24 -0.5158549320058867 2315 25 -0.38638969293049996 2316 1 -1.6408891602010485 2316 2 -0.26408674019738854 2316 3 -0.6467069511257542 2316 4 -1.3733087611935169 2316 5 -1.6597067137009809 2316 6 -1.6039220343209024 2316 7 -1.8298349098374018 2316 8 -1.6320513541577952 2316 9 -1.6091799175105947 2316 10 -1.6483788676280327 2316 11 -1.945852294153866 2316 12 -1.6871451369444443 2316 13 -1.726294585487615 2316 14 -1.9641575919998218 2316 15 -2.1187374250388444 2316 16 -1.9330122638206788 2316 17 -2.245501908927203 2316 18 -2.486249590744549 2316 19 -2.414686988508315 2316 20 -2.315999544145029 2316 21 -1.9500139895773145 2316 22 -2.053377310654014 2316 23 -1.907722308867811 2316 24 -1.6950233964063628 2316 25 -1.4762741878468901 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37576.0 3 2.0 4 305.0 5 1641.0 6 1851.0 7 1554.0 8 1451.0 9 1410.0 10 1348.0 11 1328.0 12 1260.0 13 1139.0 14 1138.0 15 1191.0 16 1298.0 17 1444.0 18 1497.0 19 1944.0 20 2433.0 21 3106.0 22 3774.0 23 4538.0 24 5228.0 25 6064.0 26 7021.0 27 7713.0 28 9060.0 29 11221.0 30 14673.0 31 19137.0 32 26057.0 33 36653.0 34 53397.0 35 81240.0 36 128524.0 37 176739.0 38 432864.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3262268568054 18.86355767120155 13.431066136795922 25.379149335197127 2 14.204680347602238 20.061785925209403 42.90717591820478 22.826357808983584 3 17.99610403565698 24.67508373751744 30.027029285859264 27.301782940966312 4 11.445061116677795 16.8600107088506 39.72561096013204 31.969317214339572 5 12.663445439508312 37.47445626867275 37.02773371882746 12.834364572991472 6 30.283022506975456 38.13033950577599 18.814255458671695 12.77238252857686 7 26.844125462345957 31.4660695243405 22.917939160920735 18.77186585239281 8 22.903623099890797 39.46845159755662 21.1871761973294 16.440749105223183 9 24.890454703674347 15.959769254577665 21.589171386612467 37.56060465513552 10 14.244763825667818 29.269305235623257 35.570073440536675 20.91585749817225 11 32.729817677837815 22.405484268272858 26.374120925396795 18.490577128492532 12 22.78544256248519 24.29965338559796 33.46821966572926 19.44668438618759 13 28.250884674128574 22.46663586877158 26.775340988722647 22.507138468377207 14 18.98888333749688 23.490194853859606 28.592066979177382 28.928854829466133 15 21.248710759088517 33.09181783198126 24.21577874743185 21.443692661498375 16 19.820006814723108 28.551852970971296 30.85370479390973 20.77443542039586 17 19.67315045016665 29.341607741966296 30.145230750014466 20.840011057852593 18 19.32578056684327 27.683944829410596 33.323076414352585 19.66719818939355 19 22.024846066804802 26.801918470513897 29.161064086173198 22.012171376508107 20 21.689830908534844 28.117988389254776 30.4471174731521 19.745063229058275 21 20.799858185081327 27.282196073820913 28.83123014338554 23.086715597712224 22 20.990743011336342 29.42728639537692 29.481474542133125 20.10049605115361 23 19.961865609337416 28.737826982653075 30.22499474182595 21.075312666183557 24 21.570035840450295 26.848832659449588 31.35644439219115 20.224687107908963 25 20.7458128395538 27.954755245718694 30.73437765500645 20.565054259721055 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 178.0 1 178.0 2 287.5 3 397.0 4 397.0 5 397.0 6 960.0 7 1523.0 8 1523.0 9 1523.0 10 2235.0 11 2947.0 12 2947.0 13 2947.0 14 4575.0 15 6203.0 16 6203.0 17 6203.0 18 10889.5 19 15576.0 20 15576.0 21 15576.0 22 24842.5 23 34109.0 24 34109.0 25 34109.0 26 49498.5 27 64888.0 28 64888.0 29 64888.0 30 85017.0 31 105146.0 32 105146.0 33 105146.0 34 114795.5 35 124445.0 36 124445.0 37 124445.0 38 128900.5 39 133356.0 40 133356.0 41 133356.0 42 137072.0 43 140788.0 44 140788.0 45 140788.0 46 139447.5 47 138107.0 48 138107.0 49 138107.0 50 127985.0 51 117863.0 52 117863.0 53 117863.0 54 100451.5 55 83040.0 56 83040.0 57 83040.0 58 69640.5 59 56241.0 60 56241.0 61 56241.0 62 45034.0 63 33827.0 64 33827.0 65 33827.0 66 25733.5 67 17640.0 68 17640.0 69 17640.0 70 12804.0 71 7968.0 72 7968.0 73 7968.0 74 5516.0 75 3064.0 76 3064.0 77 3064.0 78 2047.5 79 1031.0 80 1031.0 81 1031.0 82 681.5 83 332.0 84 332.0 85 332.0 86 218.5 87 105.0 88 105.0 89 105.0 90 67.5 91 30.0 92 30.0 93 30.0 94 19.5 95 9.0 96 9.0 97 9.0 98 7.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 6.382419851233309 3 0.0 4 0.0 5 0.0 6 9.184262949121938E-5 7 0.007163725100315111 8 0.0 9 0.0 10 0.004316603586087311 11 0.0029389641437190203 12 9.184262949121938E-5 13 0.0 14 2.7552788847365816E-4 15 0.0 16 0.0 17 0.0 18 0.011296643427419983 19 0.0032144920321926784 20 0.0 21 0.006980039841332673 22 0.0018368525898243878 23 0.0034900199206663366 24 0.009643476096578035 25 0.006796354582350235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1088819.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.43982160512154 #Duplication Level Percentage of deduplicated Percentage of total 1 90.69934076424094 70.23740768484946 2 6.647755688246044 10.296020291444114 3 1.3155869950952974 3.056364666185932 4 0.47739375374100373 1.4787714850041063 5 0.2225912603721767 0.8618713747040261 6 0.1395335175301525 0.6483270423282066 7 0.0913511777836365 0.4951953237687786 8 0.06600799880951885 0.40893181218561714 9 0.04731579474462966 0.3297714033715801 >10 0.24756715328450254 3.5129385078385864 >50 0.02056739941193381 1.0730734900481065 >100 0.020062885277343234 3.0556036918616925 >500 0.0030034216236956915 1.5557107671153916 >1k 0.0019221898391652424 2.990012459294437 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4004 0.36773788848284245 No Hit GTATCAACGCAGAGTACTTTTTTTT 3946 0.3624110159723517 No Hit CTGTAGGACGTGGAATATGGCAAGA 3231 0.29674353588612984 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3023 0.2776402689519562 No Hit CTTTAGGACGTGAAATATGGCGAGG 2443 0.22437154384704894 No Hit GGTATCAACGCAGAGTACTTTTTTT 2292 0.21050330679387486 No Hit TATCAACGCAGAGTACTTTTTTTTT 2239 0.2056356474308402 No Hit GTCCTACAGTGTGCATTTCTCATTT 1896 0.17413362551535197 No Hit CTGTAGGACCTGGAATATGGCGAGA 1284 0.11792593626672569 No Hit CTGAAGGACCTGGAATATGGCGAGA 1248 0.11461960160504178 No Hit GATATACACTGTTCTACAATGCCGG 1144 0.10506796813795496 No Hit GATATACACTGTTCTACAAATCCCG 1133 0.10405769921355155 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1089 0.10001662351593792 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 9.184262949121938E-5 0.0 0.0 0.0 5 0.0 9.184262949121938E-5 0.0 0.0 0.0 6 0.0 9.184262949121938E-5 0.0 0.0 0.0 7 0.0 9.184262949121938E-5 0.0 0.0 0.0 8 0.0 9.184262949121938E-5 0.0 0.0 0.0 9 0.0 9.184262949121938E-5 0.0 0.0 0.0 10 0.0 9.184262949121938E-5 0.0 0.0 0.0 11 0.0 9.184262949121938E-5 0.0 0.0 0.0 12 0.0 1.8368525898243877E-4 0.0 0.0 0.0 13 0.0 1.8368525898243877E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 245 0.0 16.17675 18 CCAACGA 155 0.0 15.828864 19 TACAACG 50 9.223285E-5 15.0983 18 ACCACGG 40 0.005519754 14.154007 8 CCAACGT 110 2.0736479E-10 13.725728 19 GCCGGTT 210 0.0 12.581339 11 ACGAAAT 60 4.3302038E-4 12.581339 12 TAGGACC 595 0.0 12.370456 4 GATATAC 725 0.0 12.234332 1 GGTATCA 650 0.0 11.940238 1 CCGGTTT 230 0.0 11.897571 12 GTCCTAT 170 3.6379788E-12 11.858143 1 TGTAGGA 1785 0.0 11.632273 2 ATCCCGT 165 7.2759576E-12 11.437582 10 CTGTAGG 1770 0.0 11.332231 1 CTAATGG 75 2.2060332E-4 11.323726 3 GTCCTAC 2040 0.0 11.265235 1 ACTGTTC 710 0.0 11.163724 8 GGTCTAC 100 1.2447181E-5 11.087363 1 GTAGGAC 2010 0.0 11.079599 3 >>END_MODULE