FastQCFastQC Report
Thu 26 May 2016
SRR1512164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088819
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61450.564372958223543No Hit
TATCAACGCAGAGTACTTTTTTTTT45750.4201800299223287No Hit
GGTATCAACGCAGAGTACTTTTTTT44900.4123734064155751No Hit
CTGTAGGACGTGGAATATGGCAAGA32540.2988559163644279No Hit
GTCCTACAGTGGACATTTCTAAATT25170.2311678984293992No Hit
CTTTAGGACGTGAAATATGGCGAGG23300.2139933267145412No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22560.20719697213219093No Hit
GTCCTAAAGTGTGTATTTCTCATTT21020.19305320719054314No Hit
CTGAAGGACCTGGAATATGGCGAGA15750.14465214144867053No Hit
TTGTAGAACAGTGTATATCAATGAG15050.13822315738428517No Hit
CTGTAGGACCTGGAATATGGCGAGA14680.13482498009311006No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13540.12435492033111104No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12910.11856883467316422No Hit
GTCCTACAGTGTGCATTTCTCATTT11820.1085579880586213No Hit
TTTCTAAATTTTCCACCTTTTTCAG11680.10727219124574425No Hit
GAATATGGCAAGAAAACTGAAAATC11650.1069966633572706No Hit
GATATACACTGTTCTACAAATCCCG11230.10313927291863936No Hit
GGAATATGGCGAGAAAACTGAAAAT10920.10029215140441156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT307.735399E-418.9946276
TAGGACC5900.014.3323784
GGTATCA12350.012.89047051
ACTGTTC5050.012.7884638
GTGTAGG1950.012.7103631
CCACCTT3600.012.66308413
TGTAGGA20650.012.1928762
AATCCCG2550.011.91819719
GTTATAC1209.978976E-911.8765513
CTGTAGG20100.011.8566831
GTAGGAC20300.011.7946433
GATATAC5050.011.7036031
CTAGACA907.4438813E-611.6126274
TGTAGAA7650.011.5505282
TAGACTG1406.7666406E-1011.537225
TCCAACG1406.82121E-1011.53245218
TTGTAGA5350.011.403691
CTAGACT1001.9246163E-611.4014884
CACTGTT5750.011.3967767
CGTTTCC1001.9342115E-611.39677514
Produced by FastQC (version 0.11.5)