FastQCFastQC Report
Thu 26 May 2016
SRR1512163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348947
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT49800.3691768468294159No Hit
GTATCAACGCAGAGTACTTTTTTTT48250.35768640280159264No Hit
CTGTAGGACGTGGAATATGGCAAGA42120.3122435499689758No Hit
GTCCTAAAGTGTGTATTTCTCATTT36370.26961770922059947No Hit
CTTTAGGACGTGAAATATGGCGAGG31520.23366373919805594No Hit
GGTATCAACGCAGAGTACTTTTTTT29650.21980107446771444No Hit
TATCAACGCAGAGTACTTTTTTTTT28410.21060871924545593No Hit
GTCCTACAGTGTGCATTTCTCATTT21170.15693722585097858No Hit
CTGAAGGACCTGGAATATGGCGAGA15800.11712839718684277No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15010.11127197732750063No Hit
CTGTAGGACCTGGAATATGGCGAGA14490.10741711868590834No Hit
GTCCTTCAGTGTGCATTTCTCATTT14100.10452597470471411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG556.4183623E-616.4969221
TGTGCGA350.002270743716.17542510
TCCAACG1850.014.28206518
CCAACGT955.3842086E-913.90622119
CGAAGTG500.001577681513.21042214
TAGGACC6300.013.1804664
GACCTAC1051.2553937E-712.4817971
GGTATCA10300.012.4305281
ACCTTTT4750.012.1178315
TAGACTG2050.011.96762855
GTCGAGG1201.1053999E-811.79545619
CCAACGA1201.1053999E-811.79545619
TTAGAAC803.070836E-511.7950183
ATGTCGA1451.09139364E-1011.71367517
ACTGTTC6800.011.6558218
CGAAATC1302.904926E-911.61355713
TGGCGAA752.2069362E-411.32363818
TGTCGAG1501.9826984E-1011.32363818
TATGTCG1501.9826984E-1011.32321816
GATATAC7250.011.2634151
Produced by FastQC (version 0.11.5)