FastQCFastQC Report
Thu 26 May 2016
SRR1512163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348947
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69780.5172923769429043No Hit
TATCAACGCAGAGTACTTTTTTTTT50050.37103014425325825No Hit
GGTATCAACGCAGAGTACTTTTTTT47490.35205237863311156No Hit
CTGTAGGACGTGGAATATGGCAAGA41010.30401490940711534No Hit
GTCCTACAGTGGACATTTCTAAATT34320.2544206703450914No Hit
CTTTAGGACGTGAAATATGGCGAGG31690.23492398144626883No Hit
GTCCTAAAGTGTGTATTTCTCATTT26870.19919240711458644No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24820.18399536823907833No Hit
CTGAAGGACCTGGAATATGGCGAGA19630.14552091372010909No Hit
TTGTAGAACAGTGTATATCAATGAG17240.12780339034817528No Hit
CTGTAGGACCTGGAATATGGCGAGA15410.11423725320564855No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14440.10704645920113987No Hit
GTCCTACAGTGTGCATTTCTCATTT14110.10460010660166782No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13930.10326573245650127No Hit
TTTCTAAATTTTCCACCTTTTTCAG13870.10282094107477906No Hit
GATATACACTGTTCTACAAATCCCG13750.1019313583113347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG653.3686629E-614.6164974
CTGGTCG400.005287649614.2463319
TAGGACC5500.013.4737534
CTTACAC1153.765308E-1013.2183973
CCAACGA957.405106E-812.99713319
GGTATCA15800.012.6679411
CTCGAAC550.003074007612.08779518
TCCAACG1750.011.93978118
AATCCCG3050.011.83345619
GATATAC6300.011.8004291
GGACGGG752.0783934E-411.3970646
AAATGTC4850.011.3578997
TAGGCTG1604.5474735E-1111.2821095
TACACCG600.00587164811.0841775
CCCGAAA600.005886249211.08047913
ACTGTTC5850.011.0398928
TATACAC7750.011.0330973
AATGTCC5200.010.9587158
TAGGACA5550.010.9557894
ACCGTGC700.001494388210.8543478
Produced by FastQC (version 0.11.5)