##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512163_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1348947 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.357230491635327 31.0 28.0 31.0 22.0 34.0 2 28.569948263349115 31.0 28.0 31.0 22.0 34.0 3 28.555883218540092 31.0 28.0 33.0 22.0 34.0 4 32.0707151578231 35.0 32.0 37.0 25.0 37.0 5 31.467499464397044 35.0 32.0 37.0 19.0 37.0 6 31.43311634927095 35.0 32.0 37.0 18.0 37.0 7 31.125467494275163 35.0 32.0 37.0 17.0 37.0 8 30.957996867186036 35.0 31.0 37.0 15.0 37.0 9 32.04074214924678 37.0 32.0 39.0 11.0 39.0 10 31.701985326332316 37.0 31.0 39.0 10.0 39.0 11 31.741811205332752 37.0 31.0 39.0 10.0 39.0 12 31.42165185140706 37.0 30.0 39.0 10.0 39.0 13 31.401588053496543 37.0 30.0 39.0 10.0 39.0 14 31.8876256813648 37.0 31.0 40.0 8.0 41.0 15 31.79155963874044 37.0 30.0 40.0 7.0 41.0 16 31.61497523623982 37.0 30.0 40.0 2.0 41.0 17 31.500326551006083 37.0 30.0 40.0 2.0 41.0 18 31.369403690434094 36.0 30.0 40.0 2.0 41.0 19 31.40483799585899 37.0 30.0 40.0 2.0 41.0 20 31.20195678555199 37.0 30.0 40.0 2.0 41.0 21 31.00783129359419 36.0 30.0 40.0 2.0 41.0 22 31.06682916378479 37.0 30.0 40.0 2.0 41.0 23 30.850472998568513 37.0 30.0 40.0 2.0 41.0 24 30.596088652852927 37.0 30.0 40.0 2.0 41.0 25 30.00557101205607 36.0 28.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.07357542541995699 1101 2 0.06496985503519426 1101 3 0.15049974236990238 1101 4 0.3003975661742757 1101 5 0.4594985713762334 1101 6 0.1192185693619443 1101 7 0.06971879048990104 1101 8 -0.049333354902515225 1101 9 0.14297006259164746 1101 10 0.14605097919448085 1101 11 -0.027740661254895826 1101 12 0.21518481142124557 1101 13 -0.02429098676640251 1101 14 -0.13577169010993728 1101 15 -0.05779369610002405 1101 16 -0.15482661850881385 1101 17 -0.008287515957626113 1101 18 0.0841653856269069 1101 19 -0.41395537857138365 1101 20 -0.18425182155141684 1101 21 -0.19996565309132563 1101 22 -0.0804977332893344 1101 23 -0.13058320814149127 1101 24 -0.43085568918058215 1101 25 -0.3251474080224561 1102 1 0.6406073401023846 1102 2 0.8400785404403486 1102 3 0.7072935319825149 1102 4 0.8224863564395477 1102 5 0.9085551204197309 1102 6 1.0169427930635209 1102 7 0.630418546831546 1102 8 0.8846318766454431 1102 9 1.025428020647606 1102 10 0.9754275836412454 1102 11 1.1904604729167367 1102 12 1.362260004680028 1102 13 1.2592133969084855 1102 14 1.1031694112838437 1102 15 1.3005155252084784 1102 16 1.1062549417748748 1102 17 1.455664553688056 1102 18 1.7873005833817714 1102 19 1.4029491450620277 1102 20 1.574572182422532 1102 21 1.3202866348316356 1102 22 1.222382837857456 1102 23 1.211847428996105 1102 24 1.3200213177375772 1102 25 1.1011998740265945 1103 1 0.1746620800232428 1103 2 0.21675063139636208 1103 3 0.15465402977456577 1103 4 0.31764033934945246 1103 5 0.0659216398898188 1103 6 0.023713596527418446 1103 7 -0.18210295916145824 1103 8 0.0389893492611435 1103 9 0.05689308057763398 1103 10 0.09302593771591461 1103 11 0.017079157323458105 1103 12 0.24746459836013557 1103 13 0.1903888427994076 1103 14 0.21944514678880722 1103 15 0.35351704496531866 1103 16 0.5515974605567742 1103 17 0.13317322777189844 1103 18 0.27571781398336626 1103 19 0.4125838299275415 1103 20 0.08355272666753066 1103 21 0.17788111782756033 1103 22 0.5488832393026613 1103 23 0.6526772735794601 1103 24 0.1605776777765726 1103 25 0.3823423440615805 1104 1 0.515246320102964 1104 2 0.6976942482572497 1104 3 0.6911800664807899 1104 4 0.7856078913732176 1104 5 0.8352335912914022 1104 6 0.7395832824542481 1104 7 0.7689685889203091 1104 8 0.7090700279327784 1104 9 1.232398609309758 1104 10 0.8648411710552573 1104 11 1.1220662318206855 1104 12 0.8690286761264367 1104 13 1.1123118193055177 1104 14 1.3646639977980257 1104 15 1.3832870703015097 1104 16 1.6898755188263372 1104 17 1.7259261208414038 1104 18 1.417274132770757 1104 19 1.4570186931157814 1104 20 1.4643549536047935 1104 21 1.3639247004901058 1104 22 1.4036928869126335 1104 23 1.3691215284416955 1104 24 1.4729767834715162 1104 25 1.2802648866532245 1105 1 0.130356488611028 1105 2 0.20969659847001765 1105 3 0.21240782421149973 1105 4 0.335041400546082 1105 5 0.04990981110391246 1105 6 0.13608300055664202 1105 7 0.17714992446618183 1105 8 0.11925114600173004 1105 9 0.26309163880894815 1105 10 0.3373390214146852 1105 11 0.34262803501503925 1105 12 0.1489338086731351 1105 13 0.3710522441530735 1105 14 0.3541099707742319 1105 15 0.3814330338659069 1105 16 0.2539699878765411 1105 17 0.061848465132953834 1105 18 0.5136543299437406 1105 19 0.9798505715772556 1105 20 0.7854093694371116 1105 21 0.8172850387306809 1105 22 0.6553296748698969 1105 23 0.515266932779717 1105 24 0.5112216533463041 1105 25 0.4967581352741206 1106 1 0.05005925558132773 1106 2 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1107 13 0.2220681862960312 1107 14 0.47989580172804125 1107 15 0.23750586316466382 1107 16 0.45313559471656006 1107 17 0.1858029879488292 1107 18 0.5422886314479278 1107 19 0.07476268053737556 1107 20 0.025692182709665445 1107 21 0.07132390476587958 1107 22 0.06298962282314591 1107 23 -0.041181829912215306 1107 24 0.17084736505718467 1107 25 0.08239890782221337 1108 1 -0.3037613648263964 1108 2 -0.3605312247423207 1108 3 -0.3762111464846143 1108 4 -0.27214085614761885 1108 5 -0.4338669547933378 1108 6 -0.16318191639473056 1108 7 -0.4247434115688371 1108 8 -0.3784965130082938 1108 9 -0.3423326474494246 1108 10 -0.3951882158700464 1108 11 -0.5024890473168888 1108 12 -0.47538303125774917 1108 13 -0.2713996461810879 1108 14 -0.3445181743887211 1108 15 -0.5603485012531664 1108 16 -0.48465855128192104 1108 17 -0.5174398476469477 1108 18 -0.2966752942033075 1108 19 -0.4167063767211552 1108 20 -0.13842285601837645 1108 21 -0.4087422107491534 1108 22 -0.49638392416650134 1108 23 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0.0398294316482648 2313 22 0.31895381398219413 2313 23 0.22975387694153326 2313 24 0.44692260895082825 2313 25 0.47477974246901766 2314 1 -0.36007475776347775 2314 2 -0.4100560575064769 2314 3 -0.33388736765801497 2314 4 -0.4380304996762625 2314 5 -0.4060923746416414 2314 6 -0.21749039777815327 2314 7 -0.40643700224711665 2314 8 -0.15788589433159927 2314 9 -0.6152916027210296 2314 10 -0.292912145624598 2314 11 -0.5396230790117578 2314 12 -0.40135159219810745 2314 13 -0.2938981432968326 2314 14 -0.42669716470630803 2314 15 -0.2765655816926511 2314 16 -0.6137307280978526 2314 17 -0.5131198548096307 2314 18 -0.2136841068607893 2314 19 -0.20714995141130643 2314 20 -0.8043412781817594 2314 21 -0.4225847340598463 2314 22 -0.26087236794584 2314 23 -0.32329781534470925 2314 24 -0.2673732303371672 2314 25 -0.43263076329974126 2315 1 -0.44065292575956505 2315 2 -0.5592599083162888 2315 3 -0.46800441675502213 2315 4 -0.6651564106638581 2315 5 -0.6687587571157003 2315 6 -0.9781797288051521 2315 7 -0.7701625487030448 2315 8 -0.7124986955689288 2315 9 -0.819243519518178 2315 10 -0.8086288165268272 2315 11 -0.8161001290925078 2315 12 -0.7552888894717711 2315 13 -0.9680262321835329 2315 14 -0.9917253516941145 2315 15 -0.9891990360811036 2315 16 -1.13358185489421 2315 17 -0.8915113793116767 2315 18 -1.0135810870410857 2315 19 -1.1210108992482972 2315 20 -1.3344233387967641 2315 21 -0.9260606282418244 2315 22 -1.1938930894124908 2315 23 -1.3826462491428622 2315 24 -0.992348568430728 2315 25 -1.0040956395529896 2316 1 -1.2247925680053413 2316 2 -1.1568311400572817 2316 3 -1.1305431118288922 2316 4 -1.315281368166378 2316 5 -1.431994112633216 2316 6 -1.6444209236255318 2316 7 -1.2622132832384345 2316 8 -1.484141096423052 2316 9 -1.7009737783203747 2316 10 -1.936749026240708 2316 11 -1.7667389333178 2316 12 -1.9229108796063912 2316 13 -2.0140711333806216 2316 14 -2.4231612595297563 2316 15 -2.169738768988168 2316 16 -2.237329387058093 2316 17 -1.9842498274528246 2316 18 -2.020699787721643 2316 19 -2.0052999536868263 2316 20 -1.7427808782970544 2316 21 -1.7328253323698952 2316 22 -2.0825183779480128 2316 23 -2.0415808596870093 2316 24 -2.1137124243985674 2316 25 -2.1195381799777877 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67930.0 3 2.0 4 1036.0 5 8368.0 6 10097.0 7 8118.0 8 6678.0 9 5938.0 10 5569.0 11 5186.0 12 4907.0 13 4580.0 14 4539.0 15 4508.0 16 4748.0 17 4889.0 18 5149.0 19 5874.0 20 7312.0 21 9145.0 22 11654.0 23 14108.0 24 16810.0 25 19480.0 26 22302.0 27 25108.0 28 29153.0 29 34530.0 30 42690.0 31 53235.0 32 65592.0 33 82058.0 34 102348.0 35 130546.0 36 166310.0 37 203406.0 38 155044.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.22541487925983 23.544518891830084 16.249964661051123 20.980101567858966 2 16.338002901522447 23.983225434357315 39.77005768202902 19.908713982091218 3 21.00549539752118 26.900982766557913 30.808326791193423 21.28519504472748 4 15.392450555878028 20.4135522003459 37.081812702797066 27.112184540979 5 12.850294205977553 39.65866915824092 35.62988257714797 11.861154058633565 6 30.93449927980862 39.7816222579538 17.21031293297661 12.073565529260971 7 27.383729679520396 32.90284940772321 22.321707227934084 17.39171368482231 8 24.38516857964027 40.38957794487107 19.791659716801327 15.43359375868733 9 26.06210622062987 16.054670791365414 21.02039590880887 36.862827079195846 10 15.209715429887163 31.700800698619 33.779310825406775 19.31017304608706 11 34.195413163007885 23.020696884310503 25.249843025708202 17.53404692697341 12 23.619311952211614 25.972480757212846 32.22580279284509 18.182404497730452 13 29.67069870054198 23.12388848487005 26.23994864142179 20.965464173166183 14 20.269662188358772 24.63106408183568 28.10473650929206 26.994537220513482 15 22.524606229896357 34.166724118886805 23.48765370322185 19.82101594799499 16 20.85982622000716 30.085985587276593 29.38291867656772 19.67126951614852 17 20.712674404554072 30.986836399057932 28.571915723894264 19.72857347249373 18 20.395908808870917 28.786972356956948 32.21364516174468 18.603473672427455 19 23.28786824093163 27.772254951454727 28.968298976905686 19.97157783070795 20 23.18445424468122 28.913441373159955 29.61294995281505 18.289154429343775 21 22.18812154962352 27.9139951384302 28.539964876307224 21.35791843563906 22 22.456182489008093 29.590413856141122 29.11760061737044 18.835803037480346 23 21.38364220388199 29.471951084809113 29.500788392724104 19.643618318584792 24 22.834255163471955 28.09376498854291 30.132836946151333 18.9391429018338 25 22.05206295357511 29.019762511796937 29.849940728033232 19.078233806594717 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 240.0 1 240.0 2 426.5 3 613.0 4 613.0 5 613.0 6 1475.5 7 2338.0 8 2338.0 9 2338.0 10 3449.0 11 4560.0 12 4560.0 13 4560.0 14 6603.0 15 8646.0 16 8646.0 17 8646.0 18 15083.5 19 21521.0 20 21521.0 21 21521.0 22 34315.5 23 47110.0 24 47110.0 25 47110.0 26 67753.5 27 88397.0 28 88397.0 29 88397.0 30 112248.0 31 136099.0 32 136099.0 33 136099.0 34 146576.0 35 157053.0 36 157053.0 37 157053.0 38 161267.5 39 165482.0 40 165482.0 41 165482.0 42 168764.5 43 172047.0 44 172047.0 45 172047.0 46 168580.0 47 165113.0 48 165113.0 49 165113.0 50 153397.5 51 141682.0 52 141682.0 53 141682.0 54 120107.0 55 98532.0 56 98532.0 57 98532.0 58 81714.5 59 64897.0 60 64897.0 61 64897.0 62 51828.5 63 38760.0 64 38760.0 65 38760.0 66 29586.5 67 20413.0 68 20413.0 69 20413.0 70 14998.0 71 9583.0 72 9583.0 73 9583.0 74 6747.5 75 3912.0 76 3912.0 77 3912.0 78 2640.5 79 1369.0 80 1369.0 81 1369.0 82 894.0 83 419.0 84 419.0 85 419.0 86 278.5 87 138.0 88 138.0 89 138.0 90 78.0 91 18.0 92 18.0 93 18.0 94 11.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35738987521377785 2 0.0 3 0.0 4 0.0 5 0.02980102257538658 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.005930551756295837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1348947.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.37464134508306 #Duplication Level Percentage of deduplicated Percentage of total 1 91.91352996557654 71.1177641584703 2 5.7179036295908325 8.848414851706787 3 1.1056424420040403 2.566460622178932 4 0.4020327819273138 1.2442856924236758 5 0.2306502047761178 0.8923238435361038 6 0.13475604548364276 0.6256020413027136 7 0.08627681140100936 0.4672946136985331 8 0.06439448058305311 0.39859998717733197 9 0.04783926608939834 0.3331391450291272 >10 0.24846732754094505 3.455717968451513 >50 0.02372590861444434 1.284493068607408 >100 0.019729655526218765 3.2035732223755824 >500 0.0026687068834202227 1.4810512317666236 >1k 0.0021921520828094686 3.197575081320969 >5k 1.9062192024430164E-4 0.8837044719543617 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6978 0.5172923769429043 No Hit TATCAACGCAGAGTACTTTTTTTTT 5005 0.37103014425325825 No Hit GGTATCAACGCAGAGTACTTTTTTT 4749 0.35205237863311156 No Hit CTGTAGGACGTGGAATATGGCAAGA 4101 0.30401490940711534 No Hit GTCCTACAGTGGACATTTCTAAATT 3432 0.2544206703450914 No Hit CTTTAGGACGTGAAATATGGCGAGG 3169 0.23492398144626883 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2687 0.19919240711458644 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2482 0.18399536823907833 No Hit CTGAAGGACCTGGAATATGGCGAGA 1963 0.14552091372010909 No Hit TTGTAGAACAGTGTATATCAATGAG 1724 0.12780339034817528 No Hit CTGTAGGACCTGGAATATGGCGAGA 1541 0.11423725320564855 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1444 0.10704645920113987 No Hit GTCCTACAGTGTGCATTTCTCATTT 1411 0.10460010660166782 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1393 0.10326573245650127 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1387 0.10282094107477906 No Hit GATATACACTGTTCTACAAATCCCG 1375 0.1019313583113347 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.413189695369796E-5 0.0 10 0.0 0.0 0.0 7.413189695369796E-5 0.0 11 0.0 0.0 0.0 7.413189695369796E-5 0.0 12 0.0 0.0 0.0 7.413189695369796E-5 0.0 13 0.0 0.0 0.0 2.2239569086109388E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGACG 65 3.3686629E-6 14.616497 4 CTGGTCG 40 0.0052876496 14.246331 9 TAGGACC 550 0.0 13.473753 4 CTTACAC 115 3.765308E-10 13.218397 3 CCAACGA 95 7.405106E-8 12.997133 19 GGTATCA 1580 0.0 12.667941 1 CTCGAAC 55 0.0030740076 12.087795 18 TCCAACG 175 0.0 11.939781 18 AATCCCG 305 0.0 11.833456 19 GATATAC 630 0.0 11.800429 1 GGACGGG 75 2.0783934E-4 11.397064 6 AAATGTC 485 0.0 11.357899 7 TAGGCTG 160 4.5474735E-11 11.282109 5 TACACCG 60 0.005871648 11.084177 5 CCCGAAA 60 0.0058862492 11.080479 13 ACTGTTC 585 0.0 11.039892 8 TATACAC 775 0.0 11.033097 3 AATGTCC 520 0.0 10.958715 8 TAGGACA 555 0.0 10.955789 4 ACCGTGC 70 0.0014943882 10.854347 8 >>END_MODULE