FastQCFastQC Report
Thu 26 May 2016
SRR1512162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739841
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT45880.620133244845852No Hit
CTGTAGGACGTGGAATATGGCAAGA39600.5352501415844756No Hit
GTCCTAAAGTGTGTATTTCTCATTT37190.5026755748870365No Hit
GTATCAACGCAGAGTACTTTTTTTT31620.4273891282045737No Hit
CTTTAGGACGTGAAATATGGCGAGG28390.3837310989793753No Hit
TATCAACGCAGAGTACTTTTTTTTT18500.25005372776042417No Hit
GGTATCAACGCAGAGTACTTTTTTT17500.23653731004364453No Hit
GTCCTACAGTGTGCATTTCTCATTT16610.2245076982757106No Hit
CTGAAGGACCTGGAATATGGCGAGA14950.20207044486585635No Hit
GTCCTTCAGTGTGCATTTCTCATTT13360.18057934069617662No Hit
CTGTAGGACCTGGAATATGGCGAGA12840.17355080348345117No Hit
TTTCTAAATTTTCCACCTTTTTCAG11900.16084537082967826No Hit
ATTTAGAAATGTCCACTGTAGGACG11290.15260035602244268No Hit
GAATATGGCAAGAAAACTGAAAATC10850.14665313222705958No Hit
TTCCAGGTCCTTCAGTGTGCATTTC9780.13219056527010534No Hit
ATTCCAGGTCCTTCAGTGTGCATTT9760.13192023691576973No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8950.1209719385651782No Hit
GGAATATGGCGAGAAAACTGAAAAT8360.11299725211227818No Hit
ACCTGGAATATGGCGAGAAAACTGA8220.11110495363192902No Hit
GCCATATTCCACGTCCTACAGTGGA8160.11029396856892225No Hit
ACAGTGGACATTTCTAAATTTTCCA7870.10637420743105613No Hit
GACCTGGAATATGGCGAGAAAACTG7700.10407641641920358No Hit
CCATATTTCACGTCCTAAAGTGTGT7570.10231928211602223No Hit
GATATACACTGTTCTACAAATCCCG7400.10002149110416969No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGC308.0754526E-418.8723223
CGGTAGG556.423148E-616.4933551
CCCCTAT454.3099257E-415.6788671
CCAACGA1151.8189894E-1214.77064119
TAGGACC5450.014.5438074
TAAGACC400.005516935614.1542424
TCCAACG1800.013.63093118
TTATACC500.001576324313.2106254
GTACTAG706.26141E-512.9590651
GGCCTAC550.001966120212.8281641
ATGTCGA751.5809315E-512.58239817
TATGTCG751.5809315E-512.58239816
TGTACTG604.3266886E-412.5815485
GTCGAGG701.1594314E-412.13302719
GGTATCA4750.012.0951271
TGGCGAA550.003222950712.01047118
CGGTCAG550.003222950712.01047116
TAATACT951.125296E-611.9193614
CCACCTT4850.011.8681613
CACCTTT4950.011.81903114
Produced by FastQC (version 0.11.5)