##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512162_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 739841 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.962343260241052 31.0 30.0 34.0 25.0 34.0 2 27.380981859615783 31.0 28.0 34.0 7.0 34.0 3 28.899147249206248 31.0 28.0 34.0 16.0 34.0 4 33.35236625166759 37.0 33.0 37.0 28.0 37.0 5 33.714760333639255 37.0 35.0 37.0 30.0 37.0 6 33.91459381137298 37.0 35.0 37.0 30.0 37.0 7 33.9270775747762 37.0 35.0 37.0 30.0 37.0 8 33.925055518685774 37.0 35.0 37.0 30.0 37.0 9 35.5201063471746 39.0 37.0 39.0 30.0 39.0 10 35.39465101285276 39.0 37.0 39.0 30.0 39.0 11 35.41400246809788 39.0 37.0 39.0 30.0 39.0 12 35.32848274156204 39.0 37.0 39.0 30.0 39.0 13 35.334516470430806 39.0 37.0 39.0 30.0 39.0 14 36.504178600537145 40.0 37.0 41.0 30.0 41.0 15 36.46462415573076 40.0 37.0 41.0 30.0 41.0 16 36.42720260164008 40.0 37.0 41.0 30.0 41.0 17 36.435494923909324 40.0 37.0 41.0 30.0 41.0 18 36.400050821730616 40.0 37.0 41.0 30.0 41.0 19 36.43286868394696 40.0 37.0 41.0 30.0 41.0 20 36.37412903583338 40.0 37.0 41.0 30.0 41.0 21 36.306003587257266 40.0 37.0 41.0 29.0 41.0 22 36.22791113225679 40.0 37.0 41.0 29.0 41.0 23 36.133809832112576 40.0 37.0 41.0 29.0 41.0 24 36.07396994759685 40.0 37.0 41.0 29.0 41.0 25 35.96992056401308 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4438724514627772 1101 2 3.494128704384064 1101 3 1.9993462078479531 1101 4 1.0703564826019942 1101 5 0.6714789394757261 1101 6 0.5432968097421451 1101 7 0.4191839053719377 1101 8 0.34433809501713597 1101 9 0.44481557159775775 1101 10 0.184750318038013 1101 11 0.449278406602879 1101 12 0.5470358193499365 1101 13 0.6391346911961051 1101 14 0.6108181301770585 1101 15 0.5906783847477186 1101 16 0.8024141168322032 1101 17 0.6074467444613134 1101 18 0.756688205512674 1101 19 0.5720496399209551 1101 20 0.670521937582393 1101 21 0.6346432871193386 1101 22 0.8100649678553111 1101 23 0.7627275517391183 1101 24 0.6302818351067856 1101 25 0.7483986213381968 1102 1 1.379285808332444 1102 2 4.243910993292566 1102 3 2.6518954870477174 1102 4 1.8688119106690735 1102 5 1.1244886482135925 1102 6 1.1658740383387922 1102 7 1.0164301542686687 1102 8 1.0253236790124305 1102 9 1.021925015551858 1102 10 1.1781057166846765 1102 11 1.1562230962174738 1102 12 1.3521446748956905 1102 13 1.2720031819286746 1102 14 1.33081665915617 1102 15 1.3919272814820545 1102 16 1.2483438020337303 1102 17 1.3214876388420151 1102 18 1.4426840866541895 1102 19 1.2279499047047153 1102 20 1.3251733056318216 1102 21 1.3269645580813858 1102 22 1.257412717193347 1102 23 1.1569287873966658 1102 24 1.1532106376957856 1102 25 1.1549328961248975 1103 1 0.7323918689385067 1103 2 3.7882670538986254 1103 3 2.2787515476537763 1103 4 1.108584637941803 1103 5 0.6607386482135951 1103 6 0.5157604019751503 1103 7 0.5010892451777593 1103 8 0.5111948911336412 1103 9 0.5213568337336767 1103 10 0.5954542015331583 1103 11 0.5011473386417151 1103 12 0.5305916445926542 1103 13 0.6368895455650403 1103 14 0.4433545379440531 1103 15 0.6922681905729604 1103 16 0.6844044080943377 1103 17 0.6711846085389794 1103 18 0.7145401472602444 1103 19 0.7610180865228955 1103 20 0.6941505783590927 1103 21 0.6584418308086626 1103 22 0.7282081717388067 1103 23 0.6624969692148497 1103 24 0.7415439710291167 1103 25 0.7016753203673218 1104 1 1.1885344592249325 1104 2 4.139236854645823 1104 3 2.631455470443317 1104 4 1.6703063066840187 1104 5 1.345958137628287 1104 6 1.0449774007298203 1104 7 1.0888087345924546 1104 8 0.9992879795520722 1104 9 1.1416759495493665 1104 10 1.1064411255680326 1104 11 0.9376759812320046 1104 12 1.0208702871829445 1104 13 1.083161338840256 1104 14 1.3256692951046958 1104 15 0.9846545542093281 1104 16 1.1028726521790162 1104 17 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2315 19 -0.7961253304050047 2315 20 -0.4568329639606574 2315 21 -0.5467031535173561 2315 22 -0.5308631448756174 2315 23 -0.7359865731049027 2315 24 -0.5020453706636729 2315 25 -0.556749444522957 2316 1 -1.6623624663216106 2316 2 -0.5453095356967488 2316 3 -0.5882498974329273 2316 4 -1.6388864603240947 2316 5 -1.661985340225712 2316 6 -1.6007208118976806 2316 7 -1.8208688473077856 2316 8 -1.6864857447045054 2316 9 -1.848852703838574 2316 10 -1.6366411741893856 2316 11 -2.2563237596241805 2316 12 -1.7713953496269994 2316 13 -1.7327997607933412 2316 14 -1.8253665603218465 2316 15 -1.9844731389068073 2316 16 -1.958774782657109 2316 17 -2.0949425590910735 2316 18 -2.266363031930503 2316 19 -2.2018504105869283 2316 20 -2.3172439303114274 2316 21 -1.9806549900005876 2316 22 -1.9098337357756492 2316 23 -2.0198079440799503 2316 24 -2.23669302319054 2316 25 -1.7914605177829657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27424.0 3 0.0 4 254.0 5 1097.0 6 1266.0 7 1119.0 8 958.0 9 980.0 10 912.0 11 888.0 12 857.0 13 745.0 14 792.0 15 779.0 16 884.0 17 934.0 18 971.0 19 1271.0 20 1651.0 21 2123.0 22 2478.0 23 2989.0 24 3322.0 25 3925.0 26 4315.0 27 4836.0 28 5808.0 29 7082.0 30 9043.0 31 12005.0 32 16382.0 33 23233.0 34 34505.0 35 53320.0 36 86452.0 37 118980.0 38 305261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.062582365670465 19.778574044963715 14.146147618204452 27.01269597116137 2 13.208767631206205 19.899710219421795 45.38697330178041 21.504548847591586 3 17.765033297695044 24.15072968381044 30.644827740014406 27.439409278480102 4 11.00506730500202 16.94985814519606 39.63459716344458 32.41047738635734 5 11.347708494122385 38.08683217069614 37.77406226472985 12.791397070451623 6 29.23888948961938 38.68120134083045 19.0607428633218 13.019166306228374 7 25.963519278496 31.55295841511352 23.532147047532536 18.951375258857944 8 21.80090046374829 41.08396263521487 21.3358005301139 15.779336370922941 9 24.779243107640696 15.333024257914877 22.100559444529296 37.78717318991513 10 14.213591813066277 29.06612887310712 36.10196090093037 20.618318412896233 11 31.577410721526856 21.71230839934038 28.21483604119921 18.49544483793355 12 23.564991842819857 23.769901289334573 34.11809866741278 18.547008200432796 13 28.06657106053868 23.144837877327696 27.005532269771475 21.78305879236214 14 17.850234983557232 24.593729176212662 28.73300812744394 28.82302771278616 15 19.804120074448427 35.84040354616735 23.850935538852266 20.504540840531952 16 18.1505215309776 28.356768548917945 33.35162555197671 20.141084368127746 17 18.362729289131043 30.418022250726846 31.33673316293636 19.882515297205753 18 18.313029231418096 26.977960181249934 36.06911505477511 18.639895532556856 19 22.054281431338545 26.20115137514852 29.47617702359116 22.26839016992178 20 21.570175213322862 28.180244133536803 31.43567334062319 18.81390731251715 21 19.346885146625066 27.4570208900508 29.093052239996325 24.10304172332781 22 20.491387804920908 30.981734820355676 29.619787435643392 18.907089939080024 23 18.265868451252135 30.077438069144126 31.577133264036917 20.079560215566822 24 21.54831087337667 26.577773091579576 32.90076119179032 18.97315484325343 25 19.567985509401318 28.598926722448265 32.142770245610244 19.69031752254018 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 188.0 1 188.0 2 244.5 3 301.0 4 301.0 5 301.0 6 776.0 7 1251.0 8 1251.0 9 1251.0 10 1720.0 11 2189.0 12 2189.0 13 2189.0 14 3298.0 15 4407.0 16 4407.0 17 4407.0 18 7661.0 19 10915.0 20 10915.0 21 10915.0 22 18182.0 23 25449.0 24 25449.0 25 25449.0 26 37843.5 27 50238.0 28 50238.0 29 50238.0 30 66148.0 31 82058.0 32 82058.0 33 82058.0 34 88314.5 35 94571.0 36 94571.0 37 94571.0 38 94686.5 39 94802.0 40 94802.0 41 94802.0 42 94936.0 43 95070.0 44 95070.0 45 95070.0 46 93412.5 47 91755.0 48 91755.0 49 91755.0 50 82493.5 51 73232.0 52 73232.0 53 73232.0 54 60422.5 55 47613.0 56 47613.0 57 47613.0 58 39481.0 59 31349.0 60 31349.0 61 31349.0 62 24934.0 63 18519.0 64 18519.0 65 18519.0 66 13938.0 67 9357.0 68 9357.0 69 9357.0 70 6784.0 71 4211.0 72 4211.0 73 4211.0 74 2888.5 75 1566.0 76 1566.0 77 1566.0 78 1067.5 79 569.0 80 569.0 81 569.0 82 368.0 83 167.0 84 167.0 85 167.0 86 104.0 87 41.0 88 41.0 89 41.0 90 28.0 91 15.0 92 15.0 93 15.0 94 10.0 95 5.0 96 5.0 97 5.0 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 6.3862370428240665 3 0.0 4 0.0 5 0.0 6 1.3516417716779688E-4 7 0.007163701389893235 8 0.0 9 0.0 10 0.0037845969606983124 11 0.0028384477205237344 12 2.7032835433559376E-4 13 0.0 14 2.7032835433559376E-4 15 0.0 16 0.0 17 0.0 18 0.012029611767933922 19 0.004054925315033906 20 0.0 21 0.008515343161571202 22 0.0024329551890203437 23 0.003243940252027125 24 0.01081313417342375 25 0.0068933730355576405 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 739841.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.93223054069506 #Duplication Level Percentage of deduplicated Percentage of total 1 91.35497920363598 66.62722404319982 2 5.900996101395868 8.607456161734925 3 1.1907656265179987 2.6053557957943756 4 0.4853588356328957 1.415932099813667 5 0.2571154254554192 0.9376000742441765 6 0.15462270720294596 0.6766187357130986 7 0.10639602723228445 0.5431789710703328 8 0.07877006295136238 0.4595901112699059 9 0.060370230525315816 0.39626420134205154 >10 0.33694874355689625 4.5450806794640375 >50 0.03674404932556009 1.9210426396414453 >100 0.028978189463848874 4.339605001210688 >500 0.005303198069083558 2.63627964815183 >1k 0.002651599034541779 4.288771837349647 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4588 0.620133244845852 No Hit CTGTAGGACGTGGAATATGGCAAGA 3960 0.5352501415844756 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3719 0.5026755748870365 No Hit GTATCAACGCAGAGTACTTTTTTTT 3162 0.4273891282045737 No Hit CTTTAGGACGTGAAATATGGCGAGG 2839 0.3837310989793753 No Hit TATCAACGCAGAGTACTTTTTTTTT 1850 0.25005372776042417 No Hit GGTATCAACGCAGAGTACTTTTTTT 1750 0.23653731004364453 No Hit GTCCTACAGTGTGCATTTCTCATTT 1661 0.2245076982757106 No Hit CTGAAGGACCTGGAATATGGCGAGA 1495 0.20207044486585635 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1336 0.18057934069617662 No Hit CTGTAGGACCTGGAATATGGCGAGA 1284 0.17355080348345117 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1190 0.16084537082967826 No Hit ATTTAGAAATGTCCACTGTAGGACG 1129 0.15260035602244268 No Hit GAATATGGCAAGAAAACTGAAAATC 1085 0.14665313222705958 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 978 0.13219056527010534 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 976 0.13192023691576973 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 895 0.1209719385651782 No Hit GGAATATGGCGAGAAAACTGAAAAT 836 0.11299725211227818 No Hit ACCTGGAATATGGCGAGAAAACTGA 822 0.11110495363192902 No Hit GCCATATTCCACGTCCTACAGTGGA 816 0.11029396856892225 No Hit ACAGTGGACATTTCTAAATTTTCCA 787 0.10637420743105613 No Hit GACCTGGAATATGGCGAGAAAACTG 770 0.10407641641920358 No Hit CCATATTTCACGTCCTAAAGTGTGT 757 0.10231928211602223 No Hit GATATACACTGTTCTACAAATCCCG 740 0.10002149110416969 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.3516417716779685E-4 0.0 0.0 0.0 6 0.0 1.3516417716779685E-4 0.0 0.0 0.0 7 0.0 1.3516417716779685E-4 0.0 0.0 0.0 8 0.0 2.703283543355937E-4 0.0 0.0 0.0 9 0.0 2.703283543355937E-4 0.0 0.0 0.0 10 0.0 2.703283543355937E-4 0.0 0.0 0.0 11 0.0 2.703283543355937E-4 0.0 0.0 0.0 12 0.0 2.703283543355937E-4 0.0 0.0 0.0 13 0.0 2.703283543355937E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGGC 30 8.0754526E-4 18.872322 3 CGGTAGG 55 6.423148E-6 16.493355 1 CCCCTAT 45 4.3099257E-4 15.678867 1 CCAACGA 115 1.8189894E-12 14.770641 19 TAGGACC 545 0.0 14.543807 4 TAAGACC 40 0.0055169356 14.154242 4 TCCAACG 180 0.0 13.630931 18 TTATACC 50 0.0015763243 13.210625 4 GTACTAG 70 6.26141E-5 12.959065 1 GGCCTAC 55 0.0019661202 12.828164 1 ATGTCGA 75 1.5809315E-5 12.582398 17 TATGTCG 75 1.5809315E-5 12.582398 16 TGTACTG 60 4.3266886E-4 12.581548 5 GTCGAGG 70 1.1594314E-4 12.133027 19 GGTATCA 475 0.0 12.095127 1 TGGCGAA 55 0.0032229507 12.010471 18 CGGTCAG 55 0.0032229507 12.010471 16 TAATACT 95 1.125296E-6 11.919361 4 CCACCTT 485 0.0 11.86816 13 CACCTTT 495 0.0 11.819031 14 >>END_MODULE