FastQCFastQC Report
Thu 26 May 2016
SRR1512162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739841
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52990.7162349748121556No Hit
TATCAACGCAGAGTACTTTTTTTTT41230.5572819024628264No Hit
GGTATCAACGCAGAGTACTTTTTTT40140.5425490071515366No Hit
CTGTAGGACGTGGAATATGGCAAGA35190.4756427394534772No Hit
CTTTAGGACGTGAAATATGGCGAGG24900.33655880114781417No Hit
GTCCTACAGTGGACATTTCTAAATT22390.3026325926786972No Hit
GTCCTAAAGTGTGTATTTCTCATTT19960.2697876976269225No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19630.26532727978038523No Hit
CTGAAGGACCTGGAATATGGCGAGA18440.24924274269741742No Hit
TTTCTAAATTTTCCACCTTTTTCAG14850.20071880309417833No Hit
GGAATATGGCGAGAAAACTGAAAAT13910.18801337044040545No Hit
GAATATGGCAAGAAAACTGAAAATC12850.17368596766061897No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12300.16625193791639015No Hit
ATTTAGAAATGTCCACTGTAGGACG12290.16611677373922235No Hit
ACAGTGGACATTTCTAAATTTTCCA11710.15827725146349012No Hit
CTGTAGGACCTGGAATATGGCGAGA11660.15760143057765114No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11550.15611462462880538No Hit
ACCTGGAATATGGCGAGAAAACTGA10300.13921910248283076No Hit
GAAATATGGCGAGGAAAACTGAAAA9340.12624334147472227No Hit
TTGTAGAACAGTGTATATCAATGAG9200.12435104299437312No Hit
ATTCCAGGTCCTTCAGTGTGCATTT8830.11934996843916462No Hit
GTCCTACAGTGTGCATTTCTCATTT8370.11313241628944598No Hit
TTCCAGGTCCTTCAGTGTGCATTTC7920.10705002831689513No Hit
CCACTGTAGGACGTGGAATATGGCA7880.10650937160822394No Hit
GCCATATTCCACGTCCTACAGTGGA7580.10245444629319002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACT707.2401217E-613.5732634
GGTATCA10250.013.1099491
CCACCTT4000.013.058969513
TAGGACC4950.012.8603254
TTCCACC4000.012.82153311
CACCTTT4200.012.66324314
GTGGTAT3100.012.6045661
TCCACCT4300.012.58961912
AGGCGAG701.0921644E-412.21098318
ACCTTTT4300.012.14787815
ATTGTAC550.003062727912.09254553
AGACCAG802.8576464E-511.8766065
GGACGGG802.8695353E-511.871796
TGGTCAA907.4681193E-611.60797310
GACGGGG600.00588255311.0803387
TAAGGCG600.00588255311.08033816
AAGGCGA600.00588255311.08033817
ACTGGTC600.00588255311.0803388
AATCCCG1303.263085E-810.95931519
TGTAGGA20300.010.952222
Produced by FastQC (version 0.11.5)