FastQCFastQC Report
Thu 26 May 2016
SRR1512161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928239
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63830.33102742969102894No Hit
GTCCTACAGTGGACATTTCTAAATT58110.30136305717289197No Hit
CTGTAGGACGTGGAATATGGCAAGA52940.27455102816611426No Hit
GTCCTAAAGTGTGTATTTCTCATTT44400.23026191255337125No Hit
GGTATCAACGCAGAGTACTTTTTTT39470.20469454253336855No Hit
CTTTAGGACGTGAAATATGGCGAGG38460.19945660263069048No Hit
TATCAACGCAGAGTACTTTTTTTTT35630.18477999874496887No Hit
TTGTAGAACAGTGTATATCAATGAG25300.1312078015225291No Hit
GATATACACTGTTCTACAAATCCCG23720.12301379652626049No Hit
GTCCTACAGTGTGCATTTCTCATTT22500.11668678001015434No Hit
GTGTATATCAATGAGTTACAATGAA22240.11533839944114811No Hit
CTGAAGGACCTGGAATATGGCGAGA21820.1131602462142919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGTC400.00553733114.1480119
TCCAACG5000.013.583518
TAGGACC7500.013.20515254
CCAACGA3350.012.38959319
CCAACGT2150.012.28481919
ATCCCGT3150.012.27658110
TGTCGAG1354.1836756E-1011.87857518
CGTTTCC3400.011.65190714
GGTATCA14250.011.6424561
GGCGAGG12400.011.25872519
GCCCTAA905.024202E-511.2401761
GATATAC10500.011.0796021
ACTGTTC11600.010.8955968
GTCCTAT3000.010.7905691
GACGGGG700.001583424410.77971657
CGGTAGG853.694799E-410.7112261
TGTAGGA27400.010.6699642
GTCGAGG1159.660198E-710.66337519
GTCCTAA16150.010.6485891
TGTAGAA12300.010.6096312
Produced by FastQC (version 0.11.5)