##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512161_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1928239 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.761434656181105 31.0 30.0 34.0 25.0 34.0 2 27.01403456729171 31.0 27.0 34.0 7.0 34.0 3 28.612962397296187 31.0 28.0 34.0 16.0 34.0 4 33.123113887853116 35.0 33.0 37.0 28.0 37.0 5 33.52463257926014 35.0 35.0 37.0 28.0 37.0 6 33.72978142232368 37.0 35.0 37.0 29.0 37.0 7 33.74001459362662 37.0 35.0 37.0 29.0 37.0 8 33.7469260812586 37.0 35.0 37.0 29.0 37.0 9 35.29227912100108 39.0 35.0 39.0 30.0 39.0 10 35.1681907688829 39.0 35.0 39.0 29.0 39.0 11 35.185737867556874 39.0 35.0 39.0 29.0 39.0 12 35.079620835384 39.0 35.0 39.0 29.0 39.0 13 35.08924412378341 39.0 35.0 39.0 29.0 39.0 14 36.188657630096685 40.0 36.0 41.0 29.0 41.0 15 36.12887510313815 40.0 36.0 41.0 28.0 41.0 16 36.08319456249977 40.0 36.0 41.0 28.0 41.0 17 36.08341134060664 40.0 36.0 41.0 28.0 41.0 18 36.045775445886115 40.0 36.0 41.0 28.0 41.0 19 36.07478326078873 40.0 36.0 41.0 28.0 41.0 20 35.99901568218463 40.0 36.0 41.0 27.0 41.0 21 35.936078981910434 40.0 36.0 41.0 27.0 41.0 22 35.838882005809445 40.0 36.0 41.0 27.0 41.0 23 35.72835784360756 40.0 36.0 41.0 27.0 41.0 24 35.62989909445873 40.0 36.0 41.0 27.0 41.0 25 35.51507515406544 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3836221262255073 1101 2 3.316699493958648 1101 3 1.797944978911353 1101 4 0.5339532473432271 1101 5 0.2887493175548954 1101 6 0.1125223864570799 1101 7 -0.044374385213316714 1101 8 -0.1474052207468688 1101 9 -0.23413161509858327 1101 10 -0.2720750034418842 1101 11 -0.1323531477354578 1101 12 -0.19508506696897854 1101 13 -0.29353016329846326 1101 14 -0.1807329488457654 1101 15 -0.1281166100569635 1101 16 0.0418906661767835 1101 17 -0.07751892432013108 1101 18 -0.024505107648657543 1101 19 -0.13069429785452513 1101 20 -0.1564646323603327 1101 21 -0.005980267345044865 1101 22 0.03528660534842487 1101 23 0.03626817597050547 1101 24 -0.10494959837908624 1101 25 0.012979637743001149 1102 1 1.3097923986495807 1102 2 4.214651148278179 1102 3 2.5721340524333876 1102 4 1.7659399557245195 1102 5 1.205975048790691 1102 6 1.1573356052719532 1102 7 1.2305786366376736 1102 8 0.9232329442030078 1102 9 1.020802812259916 1102 10 1.0861378100327244 1102 11 1.1394097872428475 1102 12 1.1024590334609599 1102 13 1.093990128310402 1102 14 1.2685178603589975 1102 15 1.39877014062926 1102 16 1.2306973228083251 1102 17 1.3390043483105458 1102 18 1.4328478335278163 1102 19 1.3418013486730302 1102 20 1.2808338043249279 1102 21 1.4338225228126618 1102 22 1.429757695339866 1102 23 1.3350236239600406 1102 24 1.2987557723793586 1102 25 1.2335922229677578 1103 1 1.0401172926743172 1103 2 3.979415099268248 1103 3 2.587388683407198 1103 4 1.3857873319899312 1103 5 1.017831212776521 1103 6 0.7427845726180209 1103 7 0.6740188162633203 1103 8 0.7461840891229699 1103 9 0.8125822288636755 1103 10 0.9500573462578785 1103 11 0.9435965359798573 1103 12 0.877573235113978 1103 13 0.9781388420900896 1103 14 1.0741195482207218 1103 15 1.1041618135866997 1103 16 0.9079349009590345 1103 17 1.1882741351986468 1103 18 1.1285057511864878 1103 19 1.0616709845826762 1103 20 1.1528539154295885 1103 21 1.2136383484519015 1103 22 1.3253749649525517 1103 23 1.3561335091777025 1103 24 1.4209839627670604 1103 25 1.4105520394708648 1104 1 1.0344943748655453 1104 2 3.947659452436042 1104 3 2.4562722428792654 1104 4 1.4579740886103352 1104 5 1.1769097729016806 1104 6 0.8282946824591448 1104 7 0.8930011166970857 1104 8 0.7833655117405982 1104 9 0.8378576814284386 1104 10 0.8744558081616347 1104 11 0.7853228105928665 1104 12 0.849724496458748 1104 13 0.8899284692148868 1104 14 1.015234294104168 1104 15 0.8127208365003327 1104 16 0.793433017184455 1104 17 0.8775239223969464 1104 18 0.9051554924497225 1104 19 0.8379783979482269 1104 20 0.7401827332718156 1104 21 0.9603613478852395 1104 22 0.9603825325841626 1104 23 0.9013710483208328 1104 24 0.9865654904623611 1104 25 0.9749893179592206 1105 1 0.7668857820780346 1105 2 3.5499395298424083 1105 3 2.0739810711043347 1105 4 1.104662577879182 1105 5 0.7590144556797256 1105 6 0.46912373349252334 1105 7 0.09916163297614133 1105 8 0.18734490297969586 1105 9 0.33125777922963806 1105 10 0.3681496932843231 1105 11 0.2850145137751028 1105 12 0.17321911223649522 1105 13 0.3325100755384369 1105 14 0.43095990202208156 1105 15 0.40237957074943154 1105 16 0.16282392771650223 1105 17 0.20520806529221858 1105 18 0.39724038235621606 1105 19 0.42259766037177116 1105 20 0.4154426990773672 1105 21 0.4997145792939577 1105 22 0.5283602421606446 1105 23 0.18065798692024515 1105 24 0.4416485267507255 1105 25 0.49772378002744233 1106 1 0.24658079981064773 1106 2 3.0125304208331194 1106 3 1.526058582970677 1106 4 0.4938130082688019 1106 5 0.1721781765674777 1106 6 0.12901086147715546 1106 7 -0.20359884099990921 1106 8 -0.17250338658308095 1106 9 -0.2989412787299557 1106 10 -0.28358406663334534 1106 11 -0.120651820481811 1106 12 0.0013438744064018238 1106 13 -0.20721092612949832 1106 14 -0.17875422067885438 1106 15 -0.3537593303736415 1106 16 0.003985338921118853 1106 17 -0.16065355110299606 1106 18 -0.1962070210424116 1106 19 -0.2770082597624892 1106 20 -0.13642672526454902 1106 21 -0.46752366520937727 1106 22 -0.5623976864503106 1106 23 -0.4092036137628412 1106 24 -0.4622047749815863 1106 25 -0.7630948808767997 1107 1 0.7255194438196781 1107 2 3.7393979909223916 1107 3 2.1118769128270607 1107 4 1.2216355221522477 1107 5 0.6577926617159378 1107 6 0.5266590019420292 1107 7 0.3534898514137197 1107 8 0.4306807008685354 1107 9 0.3436798934598997 1107 10 0.4572200340248358 1107 11 0.29580983275427997 1107 12 0.30276718380039824 1107 13 0.3292074454113205 1107 14 0.5377161005835234 1107 15 0.32708394197184987 1107 16 0.2968643118476564 1107 17 0.17496122622612376 1107 18 0.23758386720627556 1107 19 0.3829465301498729 1107 20 0.5054829870154123 1107 21 0.4962917077787168 1107 22 0.38911882857459545 1107 23 0.5011081235238919 1107 24 0.6192254883121464 1107 25 0.683282176318535 1108 1 0.8499599773089486 1108 2 3.4914409339598933 1108 3 2.11547080573936 1108 4 1.2251225822635092 1108 5 0.8392912822608238 1108 6 0.6380640594443179 1108 7 0.6080068172122282 1108 8 0.47122656025408105 1108 9 0.5541817452284548 1108 10 0.554810860648324 1108 11 0.4343026281001201 1108 12 0.5597834748654265 1108 13 0.4428672372935267 1108 14 0.5521703911387519 1108 15 0.6166907972640061 1108 16 0.4840887956765414 1108 17 0.4971267833310691 1108 18 0.5510762877001767 1108 19 0.42399469112628907 1108 20 0.5805602065137094 1108 21 0.48885558254818307 1108 22 0.4941785799727896 1108 23 0.52091623681914 1108 24 0.748533474157739 1108 25 0.506346440934017 1109 1 0.44827145677456315 1109 2 3.2347198387924827 1109 3 1.7345434595411078 1109 4 0.7664490708005331 1109 5 0.39614226362318306 1109 6 0.35003626792197196 1109 7 0.14210709760147466 1109 8 0.11008108771573433 1109 9 0.3076041433811838 1109 10 0.1947374646397435 1109 11 0.21106330763170433 1109 12 0.19064897049465657 1109 13 0.12570782776029432 1109 14 0.23454710233782805 1109 15 0.25461756619028364 1109 16 0.10825381299470394 1109 17 0.27152683281152434 1109 18 0.3010762877001767 1109 19 0.2113730516738741 1109 20 0.16952194162833933 1109 21 0.22633084232057143 1109 22 0.17878191165677748 1109 23 0.21827109415049506 1109 24 0.2122374985681006 1109 25 0.1600038712490388 1110 1 0.4448428859103508 1110 2 3.4666765093207985 1110 3 1.7374680902401245 1110 4 0.8524522253030113 1110 5 0.4955224246272536 1110 6 0.19783695551264913 1110 7 0.11754587862428423 1110 8 0.07488552140155491 1110 9 0.07701979006900217 1110 10 -0.016412970758629797 1110 11 0.07654140635756335 1110 12 -0.07213002371879895 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-1.2751019324718271 2315 16 -1.118008178809795 2315 17 -1.0290924535169808 2315 18 -1.0894322921146795 2315 19 -1.33977346571875 2315 20 -1.1973477109996509 2315 21 -1.222492915984219 2315 22 -1.080208038526493 2315 23 -1.0936647445912655 2315 24 -1.070321421605037 2315 25 -0.9511307924519983 2316 1 -1.5837742733022218 2316 2 -0.219612368449269 2316 3 -0.3712780293804734 2316 4 -1.2739139103502133 2316 5 -1.593608746991773 2316 6 -1.4150892137122426 2316 7 -1.4803642310754555 2316 8 -1.4401741167345747 2316 9 -1.5146287188430918 2316 10 -1.6132583635310382 2316 11 -2.1513782260756855 2316 12 -1.7113047739487968 2316 13 -1.5797572951872993 2316 14 -1.9205630550260224 2316 15 -1.9855250868957128 2316 16 -1.7892712306507903 2316 17 -1.9034365903290578 2316 18 -2.1986290968669877 2316 19 -2.0861794662944675 2316 20 -2.0404269641334025 2316 21 -2.0039992096539123 2316 22 -1.9311468551595752 2316 23 -1.9479106818377474 2316 24 -1.8694634020828858 2316 25 -1.8343204137850577 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63870.0 3 2.0 4 548.0 5 2712.0 6 3347.0 7 2920.0 8 2696.0 9 2629.0 10 2503.0 11 2408.0 12 2341.0 13 2313.0 14 2315.0 15 2488.0 16 2613.0 17 2685.0 18 3140.0 19 3937.0 20 5227.0 21 6566.0 22 8167.0 23 9826.0 24 11351.0 25 12973.0 26 14726.0 27 17374.0 28 20087.0 29 24646.0 30 31560.0 31 41463.0 32 55089.0 33 75482.0 34 106225.0 35 154761.0 36 230103.0 37 304004.0 38 693142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.20017435597973 18.66495802646871 13.28777189964522 23.84709571790634 2 14.518163695746972 20.654195626829942 42.31074292274422 22.516897754678862 3 18.758722336805757 25.135421490800674 29.978804494671046 26.127051677722523 4 11.973048984073033 17.532577652459057 39.39231599402356 31.102057369444346 5 12.340171524380535 38.65267739113253 36.442733499322436 12.564417585164495 6 30.08584011500667 38.697804625574875 18.76476738324562 12.451587876172834 7 26.6730287981536 32.36067061706625 22.63817538218171 18.328125202598446 8 23.113369245202488 39.725884602479255 21.0358778139017 16.124868338416558 9 25.298575539650425 16.501014656378178 21.372402487450984 36.82800731652041 10 14.31440194673045 30.29272527741531 35.020506639504276 20.372366136349964 11 33.05054551357418 23.054865642939493 25.76352970646458 18.131059137021747 12 22.714324017224026 24.891286703864722 33.25457399686864 19.139815282042612 13 28.435271768696722 22.876987759297474 26.587264338082573 22.100476133923234 14 19.602424184591513 23.776550173319034 28.5770517716711 28.043973870418355 15 21.80040959652823 32.966660253215494 24.198919324834733 21.034010825421536 16 20.23105019657833 29.210123848755266 30.352202190703537 20.20662376396287 17 19.977585731207174 29.75325634414045 29.60197818109401 20.66717974355836 18 19.59333961619235 28.548436538023658 32.50969788853406 19.348525957249933 19 22.234865183049212 27.464850091018945 28.70473039202975 21.595554333902093 20 21.876126351557044 28.766817806298906 30.064582243176286 19.292473598967764 21 21.408640630672682 27.849229811731757 28.513251387376176 22.22887817021939 22 21.25487889754289 29.741800910900412 29.112613719931836 19.89070647162486 23 20.873766518807106 28.991313574814814 29.353364647820925 20.78155525855716 24 21.802574662088567 27.620097301895207 30.55289882885032 20.024429207165902 25 21.395166949414943 28.425978126641837 30.055925551540664 20.12292937240256 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 253.0 1 253.0 2 430.0 3 607.0 4 607.0 5 607.0 6 1710.5 7 2814.0 8 2814.0 9 2814.0 10 4046.5 11 5279.0 12 5279.0 13 5279.0 14 8471.5 15 11664.0 16 11664.0 17 11664.0 18 20168.5 19 28673.0 20 28673.0 21 28673.0 22 45017.5 23 61362.0 24 61362.0 25 61362.0 26 88623.0 27 115884.0 28 115884.0 29 115884.0 30 152259.0 31 188634.0 32 188634.0 33 188634.0 34 204492.5 35 220351.0 36 220351.0 37 220351.0 38 228645.5 39 236940.0 40 236940.0 41 236940.0 42 243877.0 43 250814.0 44 250814.0 45 250814.0 46 247080.0 47 243346.0 48 243346.0 49 243346.0 50 226338.0 51 209330.0 52 209330.0 53 209330.0 54 178355.5 55 147381.0 56 147381.0 57 147381.0 58 122711.0 59 98041.0 60 98041.0 61 98041.0 62 77923.0 63 57805.0 64 57805.0 65 57805.0 66 43493.5 67 29182.0 68 29182.0 69 29182.0 70 20927.5 71 12673.0 72 12673.0 73 12673.0 74 8798.0 75 4923.0 76 4923.0 77 4923.0 78 3261.0 79 1599.0 80 1599.0 81 1599.0 82 1040.0 83 481.0 84 481.0 85 481.0 86 313.0 87 145.0 88 145.0 89 145.0 90 96.0 91 47.0 92 47.0 93 47.0 94 28.5 95 10.0 96 10.0 97 10.0 98 5.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 6.760935755370574 3 0.0 4 0.0 5 0.0 6 1.5558237334687246E-4 7 0.008505169742962362 8 0.0 9 0.0 10 0.004304445662596804 11 0.0029042043024749527 12 1.037215822312483E-4 13 0.0 14 1.5558237334687246E-4 15 0.0 16 0.0 17 1.5558237334687246E-4 18 0.012550311449981044 19 0.0035783945869780663 20 0.0 21 0.007208649965071757 22 0.0019188492712780936 23 0.003007925884706201 24 0.011357513254321688 25 0.007260510756187381 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1928239.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.39051069421711 #Duplication Level Percentage of deduplicated Percentage of total 1 90.55008487605525 70.0771731196262 2 6.959589999110986 10.772124485071306 3 1.2389067365170348 2.8763887512467763 4 0.4173931923521324 1.2920908926568448 5 0.22697072288693998 0.8782690078427959 6 0.1258946942984299 0.58458328112687 7 0.08825423937375315 0.47810284592451086 8 0.057530252951070324 0.3561836525000659 9 0.04402561645467214 0.3066448446949324 >10 0.24841911496478525 3.4985805894004107 >50 0.021391909105813147 1.1732810613982947 >100 0.01693422093670803 2.617063728875955 >500 0.0024376367607006197 1.318296357690743 >1k 0.0019636518350088267 2.8547978286073903 >5k 2.03136396725051E-4 0.9164195533368706 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6383 0.33102742969102894 No Hit GTCCTACAGTGGACATTTCTAAATT 5811 0.30136305717289197 No Hit CTGTAGGACGTGGAATATGGCAAGA 5294 0.27455102816611426 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4440 0.23026191255337125 No Hit GGTATCAACGCAGAGTACTTTTTTT 3947 0.20469454253336855 No Hit CTTTAGGACGTGAAATATGGCGAGG 3846 0.19945660263069048 No Hit TATCAACGCAGAGTACTTTTTTTTT 3563 0.18477999874496887 No Hit TTGTAGAACAGTGTATATCAATGAG 2530 0.1312078015225291 No Hit GATATACACTGTTCTACAAATCCCG 2372 0.12301379652626049 No Hit GTCCTACAGTGTGCATTTCTCATTT 2250 0.11668678001015434 No Hit GTGTATATCAATGAGTTACAATGAA 2224 0.11533839944114811 No Hit CTGAAGGACCTGGAATATGGCGAGA 2182 0.1131602462142919 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.186079111562415E-5 0.0 8 0.0 0.0 0.0 1.037215822312483E-4 0.0 9 0.0 0.0 0.0 1.037215822312483E-4 0.0 10 0.0 0.0 0.0 1.037215822312483E-4 0.0 11 0.0 0.0 0.0 1.5558237334687246E-4 0.0 12 0.0 0.0 0.0 1.5558237334687246E-4 0.0 13 0.0 5.186079111562415E-5 0.0 1.5558237334687246E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTGTC 40 0.005537331 14.148011 9 TCCAACG 500 0.0 13.5835 18 TAGGACC 750 0.0 13.2051525 4 CCAACGA 335 0.0 12.389593 19 CCAACGT 215 0.0 12.284819 19 ATCCCGT 315 0.0 12.276581 10 TGTCGAG 135 4.1836756E-10 11.878575 18 CGTTTCC 340 0.0 11.651907 14 GGTATCA 1425 0.0 11.642456 1 GGCGAGG 1240 0.0 11.258725 19 GCCCTAA 90 5.024202E-5 11.240176 1 GATATAC 1050 0.0 11.079602 1 ACTGTTC 1160 0.0 10.895596 8 GTCCTAT 300 0.0 10.790569 1 GACGGGG 70 0.0015834244 10.7797165 7 CGGTAGG 85 3.694799E-4 10.711226 1 TGTAGGA 2740 0.0 10.669964 2 GTCGAGG 115 9.660198E-7 10.663375 19 GTCCTAA 1615 0.0 10.648589 1 TGTAGAA 1230 0.0 10.609631 2 >>END_MODULE