FastQCFastQC Report
Thu 26 May 2016
SRR1512161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928239
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87890.45580449311522064No Hit
TATCAACGCAGAGTACTTTTTTTTT59140.30670471865780125No Hit
GGTATCAACGCAGAGTACTTTTTTT57320.29726605467475764No Hit
GTCCTACAGTGGACATTTCTAAATT56860.29488045828343895No Hit
CTGTAGGACGTGGAATATGGCAAGA53140.27558824398842674No Hit
GTCCTAAAGTGTGTATTTCTCATTT42690.2213937172725995No Hit
CTTTAGGACGTGAAATATGGCGAGG40520.21013992560050906No Hit
TTGTAGAACAGTGTATATCAATGAG31910.16548778444995668No Hit
GATATACACTGTTCTACAAATCCCG28590.14827000179956945No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27900.1446916072125914No Hit
CTGAAGGACCTGGAATATGGCGAGA23210.12036889617936365No Hit
GTCCTACAGTGTGCATTTCTCATTT22200.11513095627668563No Hit
GTGTATATCAATGAGTTACAATGAA21160.1097374340006607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG4400.015.54133818
CCAACGA3000.014.88016519
CCAACGT1750.013.5685419
GGCGTCA500.001502771513.29647912
TAGGACC7800.012.4236544
CCGTGCC550.00307499912.08770759
GGTATCA17000.011.4396971
AGGACCG752.073414E-411.4005295
GATATAC13450.011.269591
AATCCCG7500.011.14429419
CTACGGT600.00587449711.0838484
ACTGTTC12700.010.9183688
ACTGTCC2800.010.8542688
TGTAGGA29950.010.6899792
AACTGTC1605.475158E-1010.684677
GGCGAGG11950.010.65045219
CTGTAGG28000.010.5544841
CTAGACA1909.094947E-1210.5004874
GTCCTAC26200.010.4791131
TATACTG3450.010.4642535
Produced by FastQC (version 0.11.5)